PDE3B Antibody - #DF9372

(3)  
Product: PDE3B Antibody
Catalog: DF9372
Description: Rabbit polyclonal antibody to PDE3B
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 124kDa; 124kD(Calculated).
Uniprot: Q13370
RRID: AB_2842568

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(88%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(100%), Dog(88%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
PDE3B Antibody detects endogenous levels of total PDE3B.
RRID:
AB_2842568
Cite Format: Affinity Biosciences Cat# DF9372, RRID:AB_2842568.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5''-cyclic phosphodiesterase B; Adipocyte cAMP phosphodiesterase cGMP inhibited; AI847709; cAMP Phosphodiesterase, adipocyte, cGMP-inhibited; CGI-PDE B; CGIP1; CGIPDE B; CGIPDE1; cGMP inhibited 3 5 cyclic phosphodiesterase B; CGMP inhibited cAMP phophodiesterase; cGMP-inhibited 3''; Cyclic GMP inhibited phosphodiesterase B; Cyclic GMP-inhibited phosphodiesterase B; Cyclic nucleotide phosphodiesterase; HcGIP1; HcGIP1, rat, homolog of; Pde3b; PDE3B_HUMAN; Phosphodiesterase 3B; Phosphodiesterase 3B cGMP inhibited;

Immunogens

Immunogen:
Uniprot:

Q13370(PDE3B_HUMAN) >>Visit HPA database.

Gene(ID):
PDE3B(5140)
Expression:
Q13370 PDE3B_HUMAN:

Abundant in adipose tissues.

Sequence:
MRRDERDAKAMRSLQPPDGAGSPPESLRNGYVKSCVSPLRQDPPRGFFFHLCRFCNVELRPPPASPQQPRRCSPFCRARLSLGALAAFVLALLLGAEPESWAAGAAWLRTLLSVCSHSLSPLFSIACAFFFLTCFLTRTKRGPGPGRSCGSWWLLALPACCYLGDFLVWQWWSWPWGDGDAGSAAPHTPPEAAAGRLLLVLSCVGLLLTLAHPLRLRHCVLVLLLASFVWWVSFTSLGSLPSALRPLLSGLVGGAGCLLALGLDHFFQIREAPLHPRLSSAAEEKVPVIRPRRRSSCVSLGETAASYYGSCKIFRRPSLPCISREQMILWDWDLKQWYKPHYQNSGGGNGVDLSVLNEARNMVSDLLTDPSLPPQVISSLRSISSLMGAFSGSCRPKINPLTPFPGFYPCSEIEDPAEKGDRKLNKGLNRNSLPTPQLRRSSGTSGLLPVEQSSRWDRNNGKRPHQEFGISSQGCYLNGPFNSNLLTIPKQRSSSVSLTHHVGLRRAGVLSSLSPVNSSNHGPVSTGSLTNRSPIEFPDTADFLNKPSVILQRSLGNAPNTPDFYQQLRNSDSNLCNSCGHQMLKYVSTSESDGTDCCSGKSGEEENIFSKESFKLMETQQEEETEKKDSRKLFQEGDKWLTEEAQSEQQTNIEQEVSLDLILVEEYDSLIEKMSNWNFPIFELVEKMGEKSGRILSQVMYTLFQDTGLLEIFKIPTQQFMNYFRALENGYRDIPYHNRIHATDVLHAVWYLTTRPVPGLQQIHNGCGTGNETDSDGRINHGRIAYISSKSCSNPDESYGCLSSNIPALELMALYVAAAMHDYDHPGRTNAFLVATNAPQAVLYNDRSVLENHHAASAWNLYLSRPEYNFLLHLDHVEFKRFRFLVIEAILATDLKKHFDFLAEFNAKANDVNSNGIEWSNENDRLLVCQVCIKLADINGPAKVRDLHLKWTEGIVNEFYEQGDEEANLGLPISPFMDRSSPQLAKLQESFITHIVGPLCNSYDAAGLLPGQWLEAEEDNDTESGDDEDGEELDTEDEEMENNLNPKPPRRKSRRRIFCQLMHHLTENHKIWKEIVEEEEKCKADGNKLQVENSSLPQADEIQVIEEADEEE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
88
Horse
88
Bovine
88
Sheep
88
Dog
88
Xenopus
88
Chicken
63
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13370 As Substrate

Site PTM Type Enzyme
S22 Phosphorylation
S26 Phosphorylation
K33 Ubiquitination
S37 Phosphorylation
S65 Phosphorylation
S73 Phosphorylation P17612 (PRKACA)
S279 Phosphorylation
S280 Phosphorylation
K285 Ubiquitination
S295 Phosphorylation P31749 (AKT1)
S296 Phosphorylation P17612 (PRKACA)
S299 Phosphorylation
T303 Phosphorylation
S306 Phosphorylation
S310 Phosphorylation
K312 Ubiquitination
S318 Phosphorylation P17612 (PRKACA)
S323 Phosphorylation
K339 Ubiquitination
S371 Phosphorylation
S378 Phosphorylation
S379 Phosphorylation
S393 Phosphorylation
K419 Ubiquitination
K426 Ubiquitination
S432 Phosphorylation
T435 Phosphorylation
S442 Phosphorylation
T444 Phosphorylation
S445 Phosphorylation
T487 Phosphorylation
S495 Phosphorylation
S514 Phosphorylation
S518 Phosphorylation
K546 Ubiquitination
S554 Phosphorylation
T561 Phosphorylation
Y565 Phosphorylation
S571 Phosphorylation
S573 Phosphorylation
S578 Phosphorylation
K585 Ubiquitination
Y586 Phosphorylation
S588 Phosphorylation
K601 Ubiquitination
S602 Phosphorylation
K611 Ubiquitination
S613 Phosphorylation
K632 Ubiquitination
Y751 Phosphorylation
T769 Phosphorylation
T773 Phosphorylation
K943 Ubiquitination
S981 Phosphorylation
K1073 Ubiquitination
K1081 Ubiquitination

Research Backgrounds

Function:

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3. Through simultaneous binding to RAPGEF3 and PIK3R6 assembles a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Abundant in adipose tissues.

Subunit Structure:

Interacts with PIK3CG (By similarity). Interacts with RAPGEF3 and PIK3R6.

Family&Domains:

Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Morphine addiction.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Endocrine system > Renin secretion.

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