CP Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/4

Western blot analysis of CP using K562 whole cell lysates.

DF9369 at 1/100 staining mouse liver tissue by IHC-P.

DF9369 at 1/100 staining rat liver tissue by IHC-P.

DF9369 staining Hela cells by IF/ICC.

Product:	CP Antibody
Catalog:	DF9369
Description:	Rabbit polyclonal antibody to CP
Application:	WB IHC IF/ICC
Reactivity:	Human
Prediction:	Rabbit
Mol.Wt.:	122kDa; 122kD(Calculated).
Uniprot:	P00450
RRID:	AB_2842565

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Rabbit(100%)

Clonality:

Polyclonal

Specificity:

CP Antibody detects endogenous levels of total CP.

RRID:

AB_2842565
Cite Format: Affinity Biosciences Cat# DF9369, RRID:AB_2842565.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CERU_HUMAN; Ceruloplasmin; CP 2; CP; CP2; Ferroxidase;

Immunogens

Immunogen:

Uniprot:

P00450(CERU_HUMAN) >>Visit HPA database.

Gene(ID):

CP(1356)

Expression:

P00450 CERU_HUMAN:

Expressed by the liver and secreted in plasma.

Sequence:

P00450 CERU_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKILILGIFLFLCSTPAWAKEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPDRIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHSHGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGEGDGNCVTRIYHSHIDAPKDIASGLIGPLIICKKDSLDKEKEKHIDREFVVMFSVVDENFSWYLEDNIKTYCSEPEKVDKDNEDFQESNRMYSVNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVHAAFFHGQALTNKNYRIDTINLFPATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFFQVQECNKSSSKDNIRGKHVRHYYIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQGTTRIGGSYKKLVYREYTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAYPLSIEPIGVRFNKNNEGTYYSPNYNPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNADPVCLAKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENESLLLEDNIRMFTTAPDQVDKEDEDFQESNKMHSMNGFMYGNQPGLTMCKGDSVVWYLFSAGNEADVHGIYFSGNTYLWRGERRDTANLFPQTSLTLHMWPDTEGTFNVECLTTDHYTGGMKQKYTVNQCRRQSEDSTFYLGERTYYIAAVEVEWDYSPQREWEKELHHLQEQNVSNAFLDKGEFYIGSKYKKVVYRQYTDSTFRVPVERKAEEEHLGILGPQLHADVGDKVKIIFKNMATRPYSIHAHGVQTESSTVTPTLPGETLTYVWKIPERSGAGTEDSACIPWAYYSTVDQVKDLYSGLIGPLIVCRRPYLKVFNPRRKLEFALLFLVFDENESWYLDDNIKTYSDHPEKVNKDDEEFIESNKMHAINGRMFGNLQGLTMHVGDEVNWYLMGMGNEIDLHTVHFHGHSFQYKHRGVYSSDVFDIFPGTYQTLEMFPRTPGIWLLHCHVTDHIHAGMETTYTVLQNEDTKSG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

Horse

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00450 As Substrate

P00450

Site	PTM Type	Source
Y72	Phosphorylation	Uniprot
N138	N-Glycosylation	Uniprot
S204	Phosphorylation	Uniprot
K209	Ubiquitination	Uniprot
N358	N-Glycosylation	Uniprot
N397	N-Glycosylation	Uniprot
Y428	Phosphorylation	Uniprot
T429	Phosphorylation	Uniprot
S432	Phosphorylation	Uniprot
T434	Phosphorylation	Uniprot
S492	O-Glycosylation	Uniprot
S492	Phosphorylation	Uniprot
S499	Phosphorylation	Uniprot
Y539	Phosphorylation	Uniprot
N588	N-Glycosylation	Uniprot
S722	Phosphorylation	Uniprot
S725	Phosphorylation	Uniprot
N762	N-Glycosylation	Uniprot
S764	Phosphorylation	Uniprot
Y784	Phosphorylation	Uniprot
Y787	Phosphorylation	Uniprot
K906	Ubiquitination	Uniprot
N926	N-Glycosylation	Uniprot

Research Backgrounds

P00450_CERU_HUMAN

Function:

Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense (By similarity).

Subcellular Location:

Secreted.
Note: Colocalizes with GCP1 in secretory intracellular compartments.

Tissue Specificity:

Expressed by the liver and secreted in plasma.

Family&Domains:

Belongs to the multicopper oxidase family.

Research Fields

· Cellular Processes > Cell growth and death > Ferroptosis. (View pathway)

· Metabolism > Metabolism of cofactors and vitamins > Porphyrin and chlorophyll metabolism.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

CP Antibody - #DF9369