Product: CEP27 Antibody
Catalog: DF9361
Description: Rabbit polyclonal antibody to CEP27
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Prediction: Pig, Bovine, Sheep
Mol.Wt.: 27 kDa; 27kD(Calculated).
Uniprot: Q9NVX0
RRID: AB_2842557

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(88%), Bovine(88%), Sheep(88%)
Clonality:
Polyclonal
Specificity:
CEP27 Antibody detects endogenous levels of total CEP27.
RRID:
AB_2842557
Cite Format: Affinity Biosciences Cat# DF9361, RRID:AB_2842557.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C15orf25; Centrosomal protein 27 kDa; Centrosomal protein of 27 kDa; CEP27; Chromosome 15 open reading frame 25; FLJ10460; HAUS augmin-like complex subunit 2; Haus2; HAUS2_HUMAN; HsT17025;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAAANPWDPASAPNGAGLVLGHFIASGMVNQEMLNMSKKTVSCFVNFTRLQQITNIQAEIYQKNLEIELLKLEKDTADVVHPFFLAQKCHTLQSMNNHLEAVLKEKRSLRQRLLKPMCQENLPIEAVYHRYMVHLLELAVTFIERLETHLETIRNIPHLAANLKKMNQALAKMDILVTETEELAENILKWRKQQNEVSSCIPKILAEESYLYKHDIIMPPLPFTSKVHVQTINAK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Bovine
88
Sheep
88
Rabbit
75
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NVX0 As Substrate

Site PTM Type Enzyme
S37 Phosphorylation
K39 Ubiquitination
K63 Ubiquitination
K71 Ubiquitination
K74 Ubiquitination
K88 Ubiquitination
K104 Ubiquitination
K115 Ubiquitination
K192 Ubiquitination
K203 Ubiquitination
Y210 Phosphorylation
K213 Ubiquitination
K226 Ubiquitination

Research Backgrounds

Function:

Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle.
Note: Localizes to interphase centrosomes and to mitotic spindle microtubules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the HAUS augmin-like complex. The complex interacts with the gamma-tubulin ring complex and this interaction is required for spindle assembly.

Family&Domains:

Belongs to the HAUS2 family.

Restrictive clause

 

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