Product: ACAP2 Antibody
Catalog: DF9359
Description: Rabbit polyclonal antibody to ACAP2
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Horse, Sheep, Rabbit, Chicken
Mol.Wt.: 88 kDa; 88kD(Calculated).
Uniprot: Q15057
RRID: AB_2842555

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(91%)
Clonality:
Polyclonal
Specificity:
ACAP2 Antibody detects endogenous levels of total ACAP2.
RRID:
AB_2842555
Cite Format: Affinity Biosciences Cat# DF9359, RRID:AB_2842555.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ACAP 2; ACAP2; ACAP2_HUMAN; ANK repeat and PH domain-containing protein 2; Arf GAP with coiled coil ANK repeat and PH domain containing protein 2; Arf GAP with coiled coil ANK repeat and PH domains 2; Arf-GAP with coiled-coil; ArfGAP with coiled coil ankyrin repeat and PH domains 2; CENT B 2; Centaurin beta2; Centaurin-beta-2; CENTB 2; CENTB2; Cnt b2; Cnt-b2; Cntb2; KIAA0041; Similar to yeast ZINC FINGER PROTEIN GCS1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q15057 ACAP2_HUMAN:

Widely expressed. Highest level in lung.

Sequence:
MKMTVDFEECLKDSPRFRAALEEVEGDVAELELKLDKLVKLCIAMIDTGKAFCVANKQFMNGIRDLAQYSSNDAVVETSLTKFSDSLQEMINFHTILFDQTQRSIKAQLQNFVKEDLRKFKDAKKQFEKVSEEKENALVKNAQVQRNKQHEVEEATNILTATRKCFRHIALDYVLQINVLQSKRRSEILKSMLSFMYAHLAFFHQGYDLFSELGPYMKDLGAQLDRLVVDAAKEKREMEQKHSTIQQKDFSSDDSKLEYNVDAANGIVMEGYLFKRASNAFKTWNRRWFSIQNNQLVYQKKFKDNPTVVVEDLRLCTVKHCEDIERRFCFEVVSPTKSCMLQADSEKLRQAWIKAVQTSIATAYREKGDESEKLDKKSSPSTGSLDSGNESKEKLLKGESALQRVQCIPGNASCCDCGLADPRWASINLGITLCIECSGIHRSLGVHFSKVRSLTLDTWEPELLKLMCELGNDVINRVYEANVEKMGIKKPQPGQRQEKEAYIRAKYVERKFVDKYSISLSPPEQQKKFVSKSSEEKRLSISKFGPGDQVRASAQSSVRSNDSGIQQSSDDGRESLPSTVSANSLYEPEGERQDSSMFLDSKHLNPGLQLYRASYEKNLPKMAEALAHGADVNWANSEENKATPLIQAVLGGSLVTCEFLLQNGANVNQRDVQGRGPLHHATVLGHTGQVCLFLKRGANQHATDEEGKDPLSIAVEAANADIVTLLRLARMNEEMRESEGLYGQPGDETYQDIFRDFSQMASNNPEKLNRFQQDSQKF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Rabbit
100
Chicken
91
Bovine
0
Dog
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15057 As Substrate

Site PTM Type Enzyme
K57 Ubiquitination
K106 Ubiquitination
K114 Ubiquitination
K134 Ubiquitination
K140 Ubiquitination
K148 Ubiquitination
Y197 Phosphorylation
K233 Ubiquitination
K248 Ubiquitination
Y259 Phosphorylation
Y272 Phosphorylation
S278 Phosphorylation
Y298 Phosphorylation
K319 Ubiquitination
S334 Phosphorylation
K347 Ubiquitination
T358 Phosphorylation
K367 Acetylation
K377 Ubiquitination
S378 Phosphorylation
S379 Phosphorylation
S381 Phosphorylation
T382 Phosphorylation
S384 Phosphorylation
S387 Phosphorylation
K450 Ubiquitination
K515 Acetylation
S517 Phosphorylation
S519 Phosphorylation
S521 Phosphorylation
K527 Acetylation
K532 Acetylation
S540 Phosphorylation
S563 Phosphorylation
S581 Phosphorylation
S584 Phosphorylation
Y586 Phosphorylation
S595 Phosphorylation
S596 Phosphorylation
K602 Ubiquitination
K617 Acetylation
K695 Acetylation
K708 Ubiquitination
S738 Phosphorylation
Y742 Phosphorylation
T749 Phosphorylation
Y750 Phosphorylation
K767 Ubiquitination
S775 Phosphorylation

Research Backgrounds

Function:

GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6).

Subcellular Location:

Endosome membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Highest level in lung.

Subunit Structure:

Interacts with RAB35 (GTP-bound form); the interaction is direct and probably recruits ACAP2 to membranes. Interacts with MICALL1; the interaction is indirect through RAB35 (By similarity).

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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