CHST14 Antibody - #DF9331
| Product: | CHST14 Antibody |
| Catalog: | DF9331 |
| Description: | Rabbit polyclonal antibody to CHST14 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Sheep, Dog |
| Mol.Wt.: | 43 kDa; 43kD(Calculated). |
| Uniprot: | Q8NCH0 |
| RRID: | AB_2842527 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9331, RRID:AB_2842527.
Fold/Unfold
2600016L03Rik; ATCS; Carbohydrate (N acetylgalactosamine 4 0) sulfotransferase 14; Carbohydrate sulfotransferase 14; CHST14; CHSTE_HUMAN; D4ST-1; D4st1; Dermatan 4-sulfotransferase 1; hD4ST1; RP23-286G12.2; UNQ1925/PRO4400;
Immunogens
Widely expressed. Expressed at high level in pituitary gland, placenta, uterus and thyroid.
- Q8NCH0 CHSTE_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFPRPLTPLAAPNGAEPLGRALRRAPLGRARAGLGGPPLLLPSMLMFAVIVASSGLLLMIERGILAEMKPLPLHPPGREGTAWRGKAPKPGGLSLRAGDADLQVRQDVRNRTLRAVCGQPGMPRDPWDLPVGQRRTLLRHILVSDRYRFLYCYVPKVACSNWKRVMKVLAGVLDSVDVRLKMDHRSDLVFLADLRPEEIRYRLQHYFKFLFVREPLERLLSAYRNKFGEIREYQQRYGAEIVRRYRAGAGPSPAGDDVTFPEFLRYLVDEDPERMNEHWMPVYHLCQPCAVHYDFVGSYERLEADANQVLEWVRAPPHVRFPARQAWYRPASPESLHYHLCSAPRALLQDVLPKYILDFSLFAYPLPNVTKEACQQ
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8NCH0 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T7 | Phosphorylation | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| Y245 | Phosphorylation | Uniprot | |
| T259 | Phosphorylation | Uniprot |
Research Backgrounds
Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of dermatan sulfate. Plays a pivotal role in the formation of 4-0-sulfated IdoA blocks in dermatan sulfate. Transfers sulfate to the C-4 hydroxyl of beta1,4-linked GalNAc that is substituted with an alpha-linked iduronic acid (IdoUA) at the C-3 hydroxyl. Transfers sulfate more efficiently to GalNAc residues in -IdoUA-GalNAc-IdoUA- than in -GlcUA-GalNAc-GlcUA-sequences. Has preference for partially desulfated dermatan sulfate. Addition of sulfate to GalNAc may occur immediately after epimerization of GlcUA to IdoUA. Appears to have an important role in the formation of the cerebellar neural network during postnatal brain development.
Golgi apparatus membrane>Single-pass type II membrane protein.
Widely expressed. Expressed at high level in pituitary gland, placenta, uterus and thyroid.
Belongs to the sulfotransferase 2 family.
Research Fields
· Metabolism > Glycan biosynthesis and metabolism > Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.