KCNMB1 Antibody - #DF9301

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Product: KCNMB1 Antibody
Catalog: DF9301
Description: Rabbit polyclonal antibody to KCNMB1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 22 kDa; 22kD(Calculated).
Uniprot: Q16558
RRID: AB_2842497

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Bovine(90%), Horse(100%), Sheep(90%), Rabbit(80%), Dog(90%)
Clonality:
Polyclonal
Specificity:
KCNMB1 Antibody detects endogenous levels of total KCNMB1.
RRID:
AB_2842497
Cite Format: Affinity Biosciences Cat# DF9301, RRID:AB_2842497.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BK channel beta subunit; BK channel subunit beta 1; BKbeta; BKbeta1; Calcium activated potassium channel subfamily M subunit beta 1; Calcium activated potassium channel subunit beta 1; Calcium activated potassium channel subunit beta; Charybdotoxin receptor subunit beta 1; Hbeta1; hslo beta; K(VCA)beta 1; K(VCA)beta; KCNMB 1; KCNMB1; Large conductance Ca2+ activated K+ channel beta 1 subunit; Maxi K channel beta subunit; Maxi K channel subunit beta 1; Potassium large conductance calcium activated channel subfamily M beta member 1; Slo beta 1; SLO beta;

Immunogens

Immunogen:
Uniprot:

Q16558(KCMB1_HUMAN) >>Visit HPA database.

Gene(ID):
KCNMB1(3779)
Expression:
Q16558 KCMB1_HUMAN:

Abundantly expressed in smooth muscle. Low levels of expression in most other tissues. Within the brain, relatively high levels found in hippocampus and corpus callosum.

Sequence:
MVKKLVMAQKRGETRALCLGVTMVVCAVITYYILVTTVLPLYQKSVWTQESKCHLIETNIRDQEELKGKKVPQYPCLWVNVSAAGRWAVLYHTEDTRDQNQQCSYIPGSVDNYQTARADVEKVRAKFQEQQVFYCFSAPRGNETSVLFQRLYGPQALLFSLFWPTFLLTGGLLIIAMVKSNQYLSILAAQK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
90
Sheep
90
Dog
90
Rabbit
80
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Increases the apparent Ca(2+)/voltage sensitivity of the KCNMA1 channel. It also modifies KCNMA1 channel kinetics and alters its pharmacological properties. It slows down the activation and the deactivation kinetics of the channel. Acts as a negative regulator of smooth muscle contraction by enhancing the calcium sensitivity to KCNMA1. Its presence is also a requirement for internal binding of the KCNMA1 channel opener dehydrosoyasaponin I (DHS-1) triterpene glycoside and for external binding of the agonist hormone 17-beta-estradiol (E2). Increases the binding activity of charybdotoxin (CTX) toxin to KCNMA1 peptide blocker by increasing the CTX association rate and decreasing the dissociation rate.

PTMs:

N-glycosylated.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Abundantly expressed in smooth muscle. Low levels of expression in most other tissues. Within the brain, relatively high levels found in hippocampus and corpus callosum.

Subunit Structure:

Interacts with KCNMA1 tetramer. There are probably 4 molecules of KCMNB1 per KCNMA1 tetramer.

Family&Domains:

Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB1 subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

References

1). Thyrotropin induces atherosclerosis by upregulating large conductance Ca2+-activated K+ channel subunits. Molecular and cellular endocrinology, 2024 (PubMed: 38184154) [IF=4.1]
2). Fumaric acid and succinic acid treat gestational hypertension by downregulating the expression of KCNMB1 and TET1. Experimental and Therapeutic Medicine, 2021 (PubMed: 34447465) [IF=2.7]

Application: IHC    Species: Rat    Sample: placental cells

Figure 4 mRNA and protein levels of TET1 and KCNMB1 in the placenta of the control, model, DMF, succinic acid and DMF + succinic acid groups, which were examined by (A) immunohistochemical analysis, (B) reverse transcription-quantitative PCR and (C) western blotting. The protein of interest is brown in the immunohistochemical images. *P<0.05 vs. control; #P<0.05 vs. model. DMF, dimethyl fumarate; TET1, ten-eleven translocation 1; KCNMB1, calcium-activated potassium channel subunit β.

Application: WB    Species: Rat    Sample: placental cells

Figure 4 mRNA and protein levels of TET1 and KCNMB1 in the placenta of the control, model, DMF, succinic acid and DMF + succinic acid groups, which were examined by (A) immunohistochemical analysis, (B) reverse transcription-quantitative PCR and (C) western blotting. The protein of interest is brown in the immunohistochemical images. *P<0.05 vs. control; #P<0.05 vs. model. DMF, dimethyl fumarate; TET1, ten-eleven translocation 1; KCNMB1, calcium-activated potassium channel subunit β.

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