PDE1A Antibody | Affinity Biosciences

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Western blot analysis of extracts from Myeloma cells, using PDE1A Antibody.

DF9298 at 1/100 staining Human lung tissue by IHC-P.

Product:	PDE1A Antibody
Catalog:	DF9298
Description:	Rabbit polyclonal antibody to PDE1A
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	61 kDa; 61kD(Calculated).
Uniprot:	P54750
RRID:	AB_2842494

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(92%), Zebrafish(92%), Bovine(83%), Horse(100%), Sheep(83%), Rabbit(92%), Dog(92%)

Clonality:

Polyclonal

Specificity:

PDE1A Antibody detects endogenous levels of total PDE1A.

RRID:

AB_2842494
Cite Format: Affinity Biosciences Cat# DF9298, RRID:AB_2842494.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

3' 5' cyclic nucleotide phosphodiesterase; 5''-cyclic nucleotide phosphodiesterase 1A; 61 kDa Cam PDE; 61 kDa Cam-PDE; Calcium/calmodulin dependent 3' 5' cyclic nucleotide phosphodiesterase 1A; Calcium/Calmodulin stimulated cyclic nucleotide phosphodiesterase; Calcium/calmodulin-dependent 3''; Calmodulin-dependent Phosphodiesterase; Cam PDE 1A; Cam-PDE 1A; CAM-PDE-1A; HCAM 1; hCam-1; HCAM1; HSPDE1A; MGC26303; PDE 1A; PDE1A; PDE1A_HUMAN; Phosphodiesterase 1A; Phosphodiesterase 1A calmodulin dependent;

Immunogens

Immunogen:

Uniprot:

P54750(PDE1A_HUMAN) >>Visit HPA database.

Gene(ID):

PDE1A(5136)

Expression:

P54750 PDE1A_HUMAN:

Several tissues, including brain, kidney, testes and heart.

Sequence:

P54750 PDE1A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGSSATEIEELENTTFKYLTGEQTEKMWQRLKGILRCLVKQLERGDVNVVDLKKNIEYAASVLEAVYIDETRRLLDTEDELSDIQTDSVPSEVRDWLASTFTRKMGMTKKKPEEKPKFRSIVHAVQAGIFVERMYRKTYHMVGLAYPAAVIVTLKDVDKWSFDVFALNEASGEHSLKFMIYELFTRYDLINRFKIPVSCLITFAEALEVGYSKYKNPYHNLIHAADVTQTVHYIMLHTGIMHWLTELEILAMVFAAAIHDYEHTGTTNNFHIQTRSDVAILYNDRSVLENHHVSAAYRLMQEEEMNILINLSKDDWRDLRNLVIEMVLSTDMSGHFQQIKNIRNSLQQPEGIDRAKTMSLILHAADISHPAKSWKLHYRWTMALMEEFFLQGDKEAELGLPFSPLCDRKSTMVAQSQIGFIDFIVEPTFSLLTDSTEKIVIPLIEEASKAETSSYVASSSTTIVGLHIADALRRSNTKGSMSDGSYSPDYSLAAVDLKSFKNNLVDIIQQNKERWKELAAQEARTSSQKCEFIHQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Pig

Dog

Zebrafish

Rabbit

Bovine

Sheep

Chicken

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P54750 As Substrate

P54750

Site	PTM Type	Source
K53	Ubiquitination	Uniprot
Y58	Phosphorylation	Uniprot
T71	Phosphorylation	Uniprot
S82	Phosphorylation	Uniprot
T86	Phosphorylation	Uniprot
T185	Phosphorylation	Uniprot
S198	Phosphorylation	Uniprot
T202	Phosphorylation	Uniprot
S212	Phosphorylation	Uniprot
Y214	Phosphorylation	Uniprot
Y282	Phosphorylation	Uniprot
S403	Phosphorylation	Uniprot
S416	Phosphorylation	Uniprot
S487	Phosphorylation	Uniprot

Research Backgrounds

P54750_PDE1A_HUMAN

Function:

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a higher affinity for cGMP than for cAMP.

Tissue Specificity:

Several tissues, including brain, kidney, testes and heart.

Subunit Structure:

Homodimer. Interacts with YWHAZ.

Family&Domains:

Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Human Diseases > Substance dependence > Morphine addiction.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Organismal Systems > Sensory system > Olfactory transduction.

· Organismal Systems > Sensory system > Taste transduction.

· Organismal Systems > Endocrine system > Renin secretion.

Restrictive clause

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For Research Use Only.
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PDE1A Antibody - #DF9298