B4GALT4 Antibody - #DF9274

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Product: B4GALT4 Antibody
Catalog: DF9274
Description: Rabbit polyclonal antibody to B4GALT4
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.: 40 kDa; 40kD(Calculated).
Uniprot: O60513
RRID: AB_2842470

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(82%), Bovine(82%), Horse(82%), Sheep(82%), Rabbit(82%)
Clonality:
Polyclonal
Specificity:
B4GALT4 Antibody detects endogenous levels of total B4GALT4.
RRID:
AB_2842470
Cite Format: Affinity Biosciences Cat# DF9274, RRID:AB_2842470.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

B4GALT4; B4Gal T4; B4Gal-T4; Beta 1 4 galactosyltransferase 4; Beta 1 4 GalTase 4; Beta N acetylglucosaminyl glycolipid beta 1 4 galactosyltransferase 4; beta-1,4-galactosyltransferase 4; beta-1,4-GalTase 4; beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase 4; Beta4Gal T4; beta4Gal-T4; EC 2.4.1.-; UDP Gal:betaGlcNAc beta ,4 galactosyltransferase 4; UDP Gal:betaGlcNAc beta 1 4 galactosyltransferase polypeptide 4; UDP galactose:beta N acetylglucosamine beta 1 4 galactosyltransferase 4; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4; UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase 4; UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4;

Immunogens

Immunogen:
Uniprot:

O60513(B4GT4_HUMAN) >>Visit HPA database.

Gene(ID):
B4GALT4(8702)
Expression:
O60513 B4GT4_HUMAN:

High expression in heart, placenta, kidney and pancreas; lower in brain, colon, lung, muscle, ovary, testis and uterus.

Sequence:
MGFNLTFHLSYKFRLLLLLTLCLTVVGWATSNYFVGAIQEIPKAKEFMANFHKTLILGKGKTLTNEASTKKVELDNCPSVSPYLRGQSKLIFKPDLTLEEVQAENPKVSRGRYRPQECKALQRVAILVPHRNREKHLMYLLEHLHPFLQRQQLDYGIYVIHQAEGKKFNRAKLLNVGYLEALKEENWDCFIFHDVDLVPENDFNLYKCEEHPKHLVVGRNSTGYRLRYSGYFGGVTALSREQFFKVNGFSNNYWGWGGEDDDLRLRVELQRMKISRPLPEVGKYTMVFHTRDKGNEVNAERMKLLHQVSRVWRTDGLSSCSYKLVSVEHNPLYINITVDFWFGA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
82
Horse
82
Bovine
82
Sheep
82
Rabbit
82
Dog
70
Xenopus
67
Chicken
55
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60513 As Substrate

Site PTM Type Enzyme
S88 Phosphorylation
Y155 Phosphorylation
Y158 Phosphorylation
Y178 Phosphorylation

Research Backgrounds

Function:

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.

Subcellular Location:

Golgi apparatus>Golgi stack membrane>Single-pass type II membrane protein.
Note: Trans cisternae of Golgi stack.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

High expression in heart, placenta, kidney and pancreas; lower in brain, colon, lung, muscle, ovary, testis and uterus.

Family&Domains:

Belongs to the glycosyltransferase 7 family.

Research Fields

· Metabolism > Glycan biosynthesis and metabolism > Glycosaminoglycan biosynthesis - keratan sulfate.

· Metabolism > Glycan biosynthesis and metabolism > Glycosphingolipid biosynthesis - lacto and neolacto series.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). The critical role of B4GALT4 in promoting microtubule spindle assembly in HCC through the regulation of PLK1 and RHAMM expression. JOURNAL OF CELLULAR PHYSIOLOGY (PubMed: 34270095) [IF=5.6]

Application: WB    Species: Human    Sample: HCC cell

FIGURE 8 Effect of B4GALT4 knockdown on cell migration and invasion in HCC. SMMC‐7721 (a) and HepG2 (b) cells were transiently transfected with scramble siRNA (negative control) and B4GALT4 siRNA for 48h. B4GALT4 expression in two HCC cell lines were detected by western blot analysis in a nontreated control, scramble control, and B4GALT4 knockdown cells. Wound‐healing assays validating the effects of B4GALT4 knockdown on SMMC‐7721 (c) and HepG2 (d) cells migration. Scale bars, 100mm. Transwell assays validated the effects of B4GALT4 knockdown on SMMC‐7721 (e) and HepG2 (f) cells invasion. Scale bars, 200mm. (g, h) The statistical analysis of cell relative migration level. (i, j) The statistical analysis of cell relative invasiveness level. (*p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001). B4GALT, beta 1,4‐galactosyltransferase; HCC, hepatocellular carcinoma; siRNA, small interfering RNA

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