Product: B4GALT2 Antibody
Catalog: DF9273
Description: Rabbit polyclonal antibody to B4GALT2
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 42 kDa; 42kD(Calculated).
Uniprot: O60909
RRID: AB_2842469

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(86%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(88%), Chicken(86%), Xenopus(86%)
Clonality:
Polyclonal
Specificity:
B4GALT2 Antibody detects endogenous levels of total B4GALT2.
RRID:
AB_2842469
Cite Format: Affinity Biosciences Cat# DF9273, RRID:AB_2842469.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

4-galactosyltransferase 2; 4-galactosyltransferase; 4-GalTase 2; B4Gal T2; b4Gal-T2; B4GALT2; B4GT2_HUMAN; Beta 1,4 galactosyltransferase 2; Beta 1,4 GalTase 2; Beta 4 GalT2; Beta N acetylglucosaminyl glycolipid beta 1,4 galactosyltransferase 2; Beta-1; Beta-N-acetylglucosaminyl-glycolipid beta-1; Beta4Gal T2; Beta4Gal-T2; Nal synthase; UDP Gal:beta GlcNAc beta 1,4 galactosyltransferase 2; UDP Gal:betaGlcNAc beta 1,4 galactosyltransferase polypeptide 2; UDP galactose:beta N acetylglucosamine beta 1,4 galactosyltransferase 2; UDP-Gal:beta-GlcNAc beta-1; UDP-galactose:beta-N-acetylglucosamine beta-1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O60909 B4GT2_HUMAN:

Weakly expressed in various tissues. Highest expression in prostate, testis, ovary, intestine, muscle, and in fetal brain.

Sequence:
MSRLLGGTLERVCKAVLLLCLLHFLVAVILYFDVYAQHLAFFSRFSARGPAHALHPAASSSSSSSNCSRPNATASSSGLPEVPSALPGPTAPTLPPCPDSPPGLVGRLLIEFTSPMPLERVQRENPGVLMGGRYTPPDCTPAQTVAVIIPFRHREHHLRYWLHYLHPILRRQRLRYGVYVINQHGEDTFNRAKLLNVGFLEALKEDAAYDCFIFSDVDLVPMDDRNLYRCGDQPRHFAIAMDKFGFRLPYAGYFGGVSGLSKAQFLRINGFPNEYWGWGGEDDDIFNRISLTGMKISRPDIRIGRYRMIKHDRDKHNEPNPQRFTKIQNTKLTMKRDGIGSVRYQVLEVSRQPLFTNITVDIGRPPSWPPRG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Dog
88
Xenopus
86
Zebrafish
86
Chicken
86
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60909 As Substrate

Site PTM Type Enzyme
Y134 Phosphorylation
Y344 Phosphorylation
S350 Phosphorylation

Research Backgrounds

Function:

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Can produce lactose.

Subcellular Location:

Golgi apparatus>Golgi stack membrane>Single-pass type II membrane protein.
Note: Trans cisternae of Golgi stack.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Weakly expressed in various tissues. Highest expression in prostate, testis, ovary, intestine, muscle, and in fetal brain.

Family&Domains:

Belongs to the glycosyltransferase 7 family.

Research Fields

· Metabolism > Carbohydrate metabolism > Galactose metabolism.

· Metabolism > Glycan biosynthesis and metabolism > N-Glycan biosynthesis.

· Metabolism > Glycan biosynthesis and metabolism > Other types of O-glycan biosynthesis.

· Metabolism > Glycan biosynthesis and metabolism > Glycosaminoglycan biosynthesis - keratan sulfate.

· Metabolism > Glycan biosynthesis and metabolism > Glycosphingolipid biosynthesis - lacto and neolacto series.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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