B4GALT2 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from Mouse testis, using B4GALT2 Antibody.

Product:	B4GALT2 Antibody
Catalog:	DF9273
Description:	Rabbit polyclonal antibody to B4GALT2
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	42 kDa; 42kD(Calculated).
Uniprot:	O60909
RRID:	AB_2842469

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Zebrafish(86%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(88%), Chicken(86%), Xenopus(86%)

Clonality:

Polyclonal

Specificity:

B4GALT2 Antibody detects endogenous levels of total B4GALT2.

RRID:

AB_2842469
Cite Format: Affinity Biosciences Cat# DF9273, RRID:AB_2842469.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

4-galactosyltransferase 2; 4-galactosyltransferase; 4-GalTase 2; B4Gal T2; b4Gal-T2; B4GALT2; B4GT2_HUMAN; Beta 1,4 galactosyltransferase 2; Beta 1,4 GalTase 2; Beta 4 GalT2; Beta N acetylglucosaminyl glycolipid beta 1,4 galactosyltransferase 2; Beta-1; Beta-N-acetylglucosaminyl-glycolipid beta-1; Beta4Gal T2; Beta4Gal-T2; Nal synthase; UDP Gal:beta GlcNAc beta 1,4 galactosyltransferase 2; UDP Gal:betaGlcNAc beta 1,4 galactosyltransferase polypeptide 2; UDP galactose:beta N acetylglucosamine beta 1,4 galactosyltransferase 2; UDP-Gal:beta-GlcNAc beta-1; UDP-galactose:beta-N-acetylglucosamine beta-1;

Immunogens

Immunogen:

Uniprot:

O60909(B4GT2_HUMAN) >>Visit HPA database.

Gene(ID):

B4GALT2(8704)

Expression:

O60909 B4GT2_HUMAN:

Weakly expressed in various tissues. Highest expression in prostate, testis, ovary, intestine, muscle, and in fetal brain.

Sequence:

O60909 B4GT2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSRLLGGTLERVCKAVLLLCLLHFLVAVILYFDVYAQHLAFFSRFSARGPAHALHPAASSSSSSSNCSRPNATASSSGLPEVPSALPGPTAPTLPPCPDSPPGLVGRLLIEFTSPMPLERVQRENPGVLMGGRYTPPDCTPAQTVAVIIPFRHREHHLRYWLHYLHPILRRQRLRYGVYVINQHGEDTFNRAKLLNVGFLEALKEDAAYDCFIFSDVDLVPMDDRNLYRCGDQPRHFAIAMDKFGFRLPYAGYFGGVSGLSKAQFLRINGFPNEYWGWGGEDDDIFNRISLTGMKISRPDIRIGRYRMIKHDRDKHNEPNPQRFTKIQNTKLTMKRDGIGSVRYQVLEVSRQPLFTNITVDIGRPPSWPPRG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Rabbit

100

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60909 As Substrate

O60909

Site	PTM Type	Source
Y134	Phosphorylation	Uniprot
Y344	Phosphorylation	Uniprot
S350	Phosphorylation	Uniprot

Research Backgrounds

O60909_B4GT2_HUMAN

Function:

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Can produce lactose.

Subcellular Location:

Golgi apparatus>Golgi stack membrane>Single-pass type II membrane protein.
Note: Trans cisternae of Golgi stack.

Tissue Specificity:

Weakly expressed in various tissues. Highest expression in prostate, testis, ovary, intestine, muscle, and in fetal brain.

Family&Domains:

Belongs to the glycosyltransferase 7 family.

Research Fields

· Metabolism > Carbohydrate metabolism > Galactose metabolism.

· Metabolism > Glycan biosynthesis and metabolism > N-Glycan biosynthesis.

· Metabolism > Glycan biosynthesis and metabolism > Other types of O-glycan biosynthesis.

· Metabolism > Glycan biosynthesis and metabolism > Glycosaminoglycan biosynthesis - keratan sulfate.

· Metabolism > Glycan biosynthesis and metabolism > Glycosphingolipid biosynthesis - lacto and neolacto series.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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B4GALT2 Antibody - #DF9273