Product: GCNT4 Antibody
Catalog: DF9268
Description: Rabbit polyclonal antibody to GCNT4
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 53kDa; 53kD(Calculated).
Uniprot: Q9P109
RRID: AB_2842464

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(82%), Bovine(82%), Horse(91%), Sheep(82%), Dog(82%)
Clonality:
Polyclonal
Specificity:
GCNT4 Antibody detects endogenous levels of total GCNT4.
RRID:
AB_2842464
Cite Format: Affinity Biosciences Cat# DF9268, RRID:AB_2842464.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Core 2 (beta-1,6-N-acetylglucosaminyltransferase); Core 2 branching enzyme 3; Core2 GlcNAc transferase 3; GCNT4; Glucosaminyl (N-acetyl) transferase 4;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9P109 GCNT4_HUMAN:

Predominantly expressed in thymus. Weakly expressed in pancreas, peripheral blood leukocytes, placenta, small intestine and stomach. Barely detectable in liver, spleen, lung and lymph node.

Sequence:
MKIFKCYFKHTLQQKVFILFLTLWLLSLLKLLNVRRLFPQKDIYLVEYSLSTSPFVRNRYTHVKDEVRYEVNCSGIYEQEPLEIGKSLEIRRRDIIDLEDDDVVAMTSDCDIYQTLRGYAQKLVSKEEKSFPIAYSLVVHKDAIMVERLIHAIYNQHNIYCIHYDRKAPDTFKVAMNNLAKCFSNIFIASKLEAVEYAHISRLQADLNCLSDLLKSSIQWKYVINLCGQDFPLKSNFELVSELKKLNGANMLETVKPPNSKLERFTYHHELRRVPYEYVKLPIRTNISKEAPPHNIQIFVGSAYFVLSQAFVKYIFNNSIVQDFFAWSKDTYSPDEHFWATLIRVPGIPGEISRSAQDVSDLQSKTRLVKWNYYEGFFYPSCTGSHLRSVCIYGAAELRWLIKDGHWFANKFDSKVDPILIKCLAEKLEEQQRDWITLPSEKLFMDRNLTTTS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
91
Pig
82
Bovine
82
Sheep
82
Dog
82
Rabbit
73
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9P109 As Substrate

Site PTM Type Enzyme
K41 Acetylation
Y48 Phosphorylation
Y60 Phosphorylation
Y135 Phosphorylation
Y379 Phosphorylation
S381 Phosphorylation
T383 Phosphorylation
S385 Phosphorylation

Research Backgrounds

Function:

Glycosyltransferase that mediates core 2 O-glycan branching, an important step in mucin-type biosynthesis. Does not have core 4 O-glycan or I-branching enzyme activity.

Subcellular Location:

Golgi apparatus membrane>Single-pass type II membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Predominantly expressed in thymus. Weakly expressed in pancreas, peripheral blood leukocytes, placenta, small intestine and stomach. Barely detectable in liver, spleen, lung and lymph node.

Family&Domains:

Belongs to the glycosyltransferase 14 family.

Research Fields

· Metabolism > Glycan biosynthesis and metabolism > Mucin type O-glycan biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

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