Product: KCNJ15 Antibody
Catalog: DF9262
Description: Rabbit polyclonal antibody to KCNJ15
Application: WB IHC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 43 kDa; 43kD(Calculated).
Uniprot: Q99712
RRID: AB_2842458

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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(94%), Bovine(94%), Horse(94%), Sheep(94%), Rabbit(94%), Dog(94%), Chicken(94%)
Clonality:
Polyclonal
Specificity:
KCNJ15 Antibody detects endogenous levels of total KCNJ15.
RRID:
AB_2842458
Cite Format: Affinity Biosciences Cat# DF9262, RRID:AB_2842458.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP sensitive inward rectifier potassium channel 15; ATP-sensitive inward rectifier potassium channel 15; Inward rectifier K(+) channel Kir1.3; Inward rectifier K(+) channel Kir4.2; Inward rectifier K+ channel KIR4.2; inwardly rectifying subfamily J member 15; IRK15_HUMAN; IRKK; KCNJ 14; KCNJ 15; KCNJ14; Kcnj15; KIR1.3; KIR4.2; MGC13584; Potassium channel; Potassium channel inwardly rectifying subfamily J member 15; Potassium inwardly rectifying channel J15; Potassium inwardly rectifying channel subfamily J member 15;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MDAIHIGMSSTPLVKHTAGAGLKANRPRVMSKSGHSNVRIDKVDGIYLLYLQDLWTTVIDMKWRYKLTLFAATFVMTWFLFGVIYYAIAFIHGDLEPGEPISNHTPCIMKVDSLTGAFLFSLESQTTIGYGVRSITEECPHAIFLLVAQLVITTLIEIFITGTFLAKIARPKKRAETIKFSHCAVITKQNGKLCLVIQVANMRKSLLIQCQLSGKLLQTHVTKEGERILLNQATVKFHVDSSSESPFLILPMTFYHVLDETSPLRDLTPQNLKEKEFELVVLLNATVESTSAVCQSRTSYIPEEIYWGFEFVPVVSLSKNGKYVADFSQFEQIRKSPDCTFYCADSEKQQLEEKYRQEDQRERELRTLLLQQSNV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
94
Horse
94
Bovine
94
Sheep
94
Dog
94
Chicken
94
Rabbit
94
Zebrafish
67
Xenopus
53
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99712 As Substrate

Site PTM Type Enzyme
K348 Ubiquitination

Research Backgrounds

Function:

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with PATJ.

Family&Domains:

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ15 subfamily.

Research Fields

· Organismal Systems > Digestive system > Gastric acid secretion.

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