KCNJ10 Antibody - #DF9260

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Product: KCNJ10 Antibody
Catalog: DF9260
Description: Rabbit polyclonal antibody to KCNJ10
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 43 kDa; 43kD(Calculated).
Uniprot: P78508
RRID: AB_2842456

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
KCNJ10 Antibody detects endogenous levels of total KCNJ10.
RRID:
AB_2842456
Cite Format: Affinity Biosciences Cat# DF9260, RRID:AB_2842456.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

inwardly rectifying subfamily J member 10; ATP dependent inwardly rectifying potassium channel Kir4.1; ATP sensitive inward rectifier potassium channel 10; ATP-dependent inwardly rectifying potassium channel Kir4.1; ATP-sensitive inward rectifier potassium channel 10; BIRK10; Glial ATP dependent inwardly rectifying potassium channel KIR4.1; Inward rectifier K(+) channel Kir1.2; Inward rectifier K+ channel KIR1.2; Inwardly rectifying potassium channel Kir1.2; KCJ10_HUMAN; KCNJ 10; Kcnj10; KCNJ13 PEN; KIR1.2; KIR4.1; Potassium channel; Potassium channel inwardly rectifying subfamily J member 10; Potassium inwardly rectifying channel subfamily J member 10; SESAME;

Immunogens

Immunogen:
Uniprot:

P78508(KCJ10_HUMAN) >>Visit HPA database.

Gene(ID):
KCNJ10(3766)
Expression:
P78508 KCJ10_HUMAN:

Expressed in kidney (at protein level).

Sequence:
MTSVAKVYYSQTTQTESRPLMGPGIRRRRVLTKDGRSNVRMEHIADKRFLYLKDLWTTFIDMQWRYKLLLFSATFAGTWFLFGVVWYLVAVAHGDLLELDPPANHTPCVVQVHTLTGAFLFSLESQTTIGYGFRYISEECPLAIVLLIAQLVLTTILEIFITGTFLAKIARPKKRAETIRFSQHAVVASHNGKPCLMIRVANMRKSLLIGCQVTGKLLQTHQTKEGENIRLNQVNVTFQVDTASDSPFLILPLTFYHVVDETSPLKDLPLRSGEGDFELVLILSGTVESTSATCQVRTSYLPEEILWGYEFTPAISLSASGKYIADFSLFDQVVKVASPSGLRDSTVRYGDPEKLKLEESLREQAEKEGSALSVRISNV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
80
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P78508 As Substrate

Site PTM Type Enzyme
Y8 Phosphorylation
Y9 Phosphorylation
T32 Phosphorylation
K224 Ubiquitination
S377 Phosphorylation

Research Backgrounds

Function:

May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium (By similarity). In the kidney, together with KCNJ16, mediates basolateral K(+) recycling in distal tubules; this process is critical for Na(+) reabsorption at the tubules.

Subcellular Location:

Membrane>Multi-pass membrane protein. Basolateral cell membrane.
Note: In kidney distal convoluted tubules, located in the basolateral membrane where it colocalizes with KCNJ16.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in kidney (at protein level).

Subunit Structure:

Heterodimer with Kir5.1/KCNJ16; this interaction is required for KCNJ16 localization to the basolateral membrane in kidney cells. Interacts with MAGI1, alone and possibly as a heterodimer with KCNJ16; this interaction may facilitate KCNJ10/KCNJ16 potassium channel expression at the basolateral membrane in kidney cells. Interacts with PATJ (By similarity).

Family&Domains:

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ10 subfamily.

Research Fields

· Organismal Systems > Digestive system > Gastric acid secretion.

References

1). Inhibitory effect of tumor necrosis factor‑α on the basolateral Kir4. 1/Kir5. 1 channels in the thick ascending limb during diabetes. Experimental and Therapeutic Medicine, 2021 (PubMed: 34539838) [IF=2.7]

Application: WB    Species: Rat    Sample: kidney tissues

Figure 2 Effect of TNF-α on the basolateral Kir4.1/Kir5.1 channels in the thick ascending limb during diabetes. **P<0.01 vs. control group; ##P<0.01 vs. diabetic group. TNFR:Fc, TNF receptor fusion protein; Kir, inwardly rectifying potassium channels.

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