DDX42 Antibody - #DF9259

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Product: DDX42 Antibody
Catalog: DF9259
Description: Rabbit polyclonal antibody to DDX42
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 103 kDa; 103kD(Calculated).
Uniprot: Q86XP3
RRID: AB_2842455

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
DDX42 Antibody detects endogenous levels of total DDX42.
RRID:
AB_2842455
Cite Format: Affinity Biosciences Cat# DF9259, RRID:AB_2842455.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP-dependent RNA helicase DDX42; DEAD (Asp-Glu-Ala-Asp) box polypeptide 42; DEAD box polypeptide 42 protein; DEAD box protein 42; EC 3.6.1.-; FLJ43179; RHELP; RNA helicase-like protein; RNA helicase-related protein; RNAHP; SF3b DEAD-box protein; SF3b125; SF3b125 DEAD-box protein; Splicing factor 3B-associated 125 kDa protein;

Immunogens

Immunogen:
Uniprot:

Q86XP3(DDX42_HUMAN) >>Visit HPA database.

Gene(ID):
DDX42(11325)
Expression:
Q86XP3 DDX42_HUMAN:

Expressed in several cell lines (at protein level). Expressed in liver, lung, tonsil, thymus, muscle and pancreatic islets.

Sequence:
MNWNKGGPGTKRGFGFGGFAISAGKKEEPKLPQQSHSAFGATSSSSGFGKSAPPQLPSFYKIGSKRANFDEENAYFEDEEEDSSNVDLPYIPAENSPTRQQFHSKPVDSDSDDDPLEAFMAEVEDQAARDMKRLEEKDKERKNVKGIRDDIEEEDDQEAYFRYMAENPTAGVVQEEEEDNLEYDSDGNPIAPTKKIIDPLPPIDHSEIDYPPFEKNFYNEHEEITNLTPQQLIDLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQCQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELEPGDGPIAVIVCPTRELCQQIHAECKRFGKAYNLRSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRMFDMGFEYQVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEANEDVTQIVEILHSGPSKWNWLTRRLVEFTSSGSVLLFVTKKANAEELANNLKQEGHNLGLLHGDMDQSERNKVISDFKKKDIPVLVATDVAARGLDIPSIKTVINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLDLAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLGSENMDRGNNNVMSNYEAYKPSTGAMGDRLTAMKAAFQSQYKSHFVAASLSNQKAGSSAAGASGWTSAGSLNSVPTNSAQQGHNSPDSPVTSAAKGIPGFGNTGNISGAPVTYPSAGAQGVNNTASGNNSREGTGGSNGKRERYTENRGSSRHSHGETGNRHSDSPRHGDGGRHGDGYRHPESSSRHTDGHRHGENRHGGSAGRHGENRGANDGRNGESRKEAFNRESKMEPKMEPKVDSSKMDKVDSKTDKTADGFAVPEPPKRKKSRWDS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Xenopus
64
Chicken
64
Sheep
58
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q86XP3 As Substrate

Site PTM Type Enzyme
K5 Acetylation
T10 Phosphorylation
R12 Methylation
K25 Acetylation
K26 Acetylation
K30 Acetylation
K30 Ubiquitination
K50 Methylation
S51 Phosphorylation
S58 Phosphorylation
S96 Phosphorylation
T98 Phosphorylation
S104 Phosphorylation
S109 Phosphorylation
S111 Phosphorylation
K145 Acetylation
Y160 Phosphorylation
Y183 Phosphorylation
S185 Phosphorylation
T193 Phosphorylation
K195 Ubiquitination
K215 Ubiquitination
T228 Phosphorylation
K270 Sumoylation
K270 Ubiquitination
T555 Phosphorylation
T581 Phosphorylation
T583 Phosphorylation
T600 Phosphorylation
T603 Phosphorylation
K605 Ubiquitination
K626 Acetylation
R673 Methylation
Y682 Phosphorylation
K686 Acetylation
K700 Methylation
K700 Ubiquitination
S705 Phosphorylation
Y707 Phosphorylation
K708 Methylation
S715 Phosphorylation
S717 Phosphorylation
T742 Phosphorylation
S744 Phosphorylation
S751 Phosphorylation
S754 Phosphorylation
T757 Phosphorylation
S758 Phosphorylation
K806 Acetylation
R814 Methylation
S820 Phosphorylation
S831 Phosphorylation
Y844 Phosphorylation
R875 Methylation
S894 Phosphorylation
K899 Sumoylation
S914 Phosphorylation
S938 Phosphorylation

Research Backgrounds

Function:

ATP-dependent RNA helicase. Binds to partially double-stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. Unwinding is promoted in the presence of single-strand binding proteins. Mediates also RNA duplex formation thereby displacing the single-strand RNA binding protein. ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP-bound form of the protein triggers annealing of complementary RNA strands. Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis-stimulating activity of TP53BP2. Relocalizes TP53BP2 to the cytoplasm.

Subcellular Location:

Cytoplasm. Nucleus speckle. Nucleus>Cajal body.
Note: Isoform 2 is present in Cajal bodies (CBs) and nuclear speckles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in several cell lines (at protein level). Expressed in liver, lung, tonsil, thymus, muscle and pancreatic islets.

Subunit Structure:

Component of splicing factor SF3B complex which is composed of at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A/SF3B14B, and DDX42/SF3B125. Interacts (via the C-terminus) with TP53BP2; the interaction is not inhibitied by TP53BP2 ubiquitination and is independent of p53/TP53.

Family&Domains:

Belongs to the DEAD box helicase family. DDX42 subfamily.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

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