ABCC9 Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/3

Western blot analysis of extracts from HepG2 cells(heat-shock treatment), using ABCC9 Antibody.

Figure 4 The mRNA level and protein expression of ABCC9.

Product:	ABCC9 Antibody
Catalog:	DF9255
Description:	Rabbit polyclonal antibody to ABCC9
Application:	WB IHC IF/ICC
Reactivity:	Human, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	174kDa; 174kD(Calculated).
Uniprot:	O60706
RRID:	AB_2842451

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

ABCC9 Antibody detects endogenous levels of total ABCC9.

RRID:

AB_2842451
Cite Format: Affinity Biosciences Cat# DF9255, RRID:AB_2842451.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ABC37; abcC9; ABCC9_HUMAN; AI414027; AI449286; ATFB12; ATP-binding cassette sub-family C member 9; ATP-binding cassette transporter sub-family C member 9; ATP-binding cassette, sub-family C (CFTR/MRP), member 9; CANTU; CMD1O; FLJ36852; Sulfonylurea receptor 2; Sulfonylurea-binding protein 2; SUR2; SUR2A; SUR2B;

Immunogens

Immunogen:

Uniprot:

O60706(ABCC9_HUMAN) >>Visit HPA database.

Gene(ID):

ABCC9(10060)

Sequence:

O60706 ABCC9_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSLSFCGNNISSYNINDGVLQNSCFVDALNLVPHVFLLFITFPILFIGWGSQSSKVQIHHNTWLHFPGHNLRWILTFALLFVHVCEIAEGIVSDSRRESRHLHLFMPAVMGFVATTTSIVYYHNIETSNFPKLLLALFLYWVMAFITKTIKLVKYCQSGLDISNLRFCITGMMVILNGLLMAVEINVIRVRRYVFFMNPQKVKPPEDLQDLGVRFLQPFVNLLSKATYWWMNTLIISAHKKPIDLKAIGKLPIAMRAVTNYVCLKDAYEEQKKKVADHPNRTPSIWLAMYRAFGRPILLSSTFRYLADLLGFAGPLCISGIVQRVNETQNGTNNTTGISETLSSKEFLENAYVLAVLLFLALILQRTFLQASYYVTIETGINLRGALLAMIYNKILRLSTSNLSMGEMTLGQINNLVAIETNQLMWFLFLCPNLWAMPVQIIMGVILLYNLLGSSALVGAAVIVLLAPIQYFIATKLAEAQKSTLDYSTERLKKTNEILKGIKLLKLYAWEHIFCKSVEETRMKELSSLKTFALYTSLSIFMNAAIPIAAVLATFVTHAYASGNNLKPAEAFASLSLFHILVTPLFLLSTVVRFAVKAIISVQKLNEFLLSDEIGDDSWRTGESSLPFESCKKHTGVQPKTINRKQPGRYHLDSYEQSTRRLRPAETEDIAIKVTNGYFSWGSGLATLSNIDIRIPTGQLTMIVGQVGCGKSSLLLAILGEMQTLEGKVHWSNVNESEPSFEATRSRNRYSVAYAAQKPWLLNATVEENITFGSPFNKQRYKAVTDACSLQPDIDLLPFGDQTEIGERGINLSGGQRQRICVARALYQNTNIVFLDDPFSALDIHLSDHLMQEGILKFLQDDKRTLVLVTHKLQYLTHADWIIAMKDGSVLREGTLKDIQTKDVELYEHWKTLMNRQDQELEKDMEADQTTLERKTLRRAMYSREAKAQMEDEDEEEEEEEDEDDNMSTVMRLRTKMPWKTCWRYLTSGGFFLLILMIFSKLLKHSVIVAIDYWLATWTSEYSINNTGKADQTYYVAGFSILCGAGIFLCLVTSLTVEWMGLTAAKNLHHNLLNKIILGPIRFFDTTPLGLILNRFSADTNIIDQHIPPTLESLTRSTLLCLSAIGMISYATPVFLVALLPLGVAFYFIQKYFRVASKDLQELDDSTQLPLLCHFSETAEGLTTIRAFRHETRFKQRMLELTDTNNIAYLFLSAANRWLEVRTDYLGACIVLTASIASISGSSNSGLVGLGLLYALTITNYLNWVVRNLADLEVQMGAVKKVNSFLTMESENYEGTMDPSQVPEHWPQEGEIKIHDLCVRYENNLKPVLKHVKAYIKPGQKVGICGRTGSGKSSLSLAFFRMVDIFDGKIVIDGIDISKLPLHTLRSRLSIILQDPILFSGSIRFNLDPECKCTDDRLWEALEIAQLKNMVKSLPGGLDAVVTEGGENFSVGQRQLFCLARAFVRKSSILIMDEATASIDMATENILQKVVMTAFADRTVVTIAHRVSSIMDAGLVLVFSEGILVECDTVPNLLAHKNGLFSTLVMTNK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Zebrafish

Chicken

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60706 As Substrate

O60706

Site	PTM Type	Source
T170	Phosphorylation	Uniprot
S237	Phosphorylation	Uniprot
T635	Phosphorylation	Uniprot
T771	Phosphorylation	Uniprot
Y1321	Phosphorylation	Uniprot
S1350	Phosphorylation	Uniprot
S1378	Phosphorylation	Uniprot
T1414	Phosphorylation	Uniprot
S1468	Phosphorylation	Uniprot

Research Backgrounds

O60706_ABCC9_HUMAN

Function:

Subunit of ATP-sensitive potassium channels (KATP). Can form cardiac and smooth muscle-type KATP channels with KCNJ11. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Subunit Structure:

Interacts with KCNJ11.

Family&Domains:

Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.

Research Fields

· Environmental Information Processing > Membrane transport > ABC transporters.

References

1). Knocking Out of CEACAM1 Can Reduce Oxidative Stress and Promote Cell Proliferation in the HPMVECs under Hypoxia. Journal of Immunology Research, 2022 (PubMed: 35812245) [IF=4.1]

2). ABCC9 Is Downregulated and Prone to Microsatellite Instability on ABCC9tetra in Canine Breast Cancer. Frontiers in Veterinary Science, 2022 (PubMed: 35071399) [IF=3.2]

Application: WB Species: Human Sample: Canine Breast Cancer

Figure 4 The mRNA level and protein expression of ABCC9. (A) The mRNA level of ABCC9, the mRNA level was expression by 2−ΔΔCt. (B) Average optical density values of ABCC9 protein in CMTs. AOD = IOD/Area, all of data are shown as means ± SD, *indicates that there is significant difference between two groups (P < 0.05). (C) IHC staining of ABCC9 in paracancer tissue (200×). (D) IHC staining of ABCC9 in benign tumor (200×). (E) IHC staining of ABCC9 in complex breast cancer (200×). (F) No strong positive staining of ABCC9 in higher malignancy cells (200×). Strongly positive cells can be observed in para-cancer tissues and benign tumors, but was significant decreased in malignant tumors.

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For Research Use Only.
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ABCC9 Antibody - #DF9255