Product: ABCA5 Antibody
Catalog: DF9246
Description: Rabbit polyclonal antibody to ABCA5
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Horse, Rabbit
Mol.Wt.: 187kDa; 187kD(Calculated).
Uniprot: Q8WWZ7
RRID: AB_2842442

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(100%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
ABCA5 Antibody detects endogenous levels of total ABCA5.
RRID:
AB_2842442
Cite Format: Affinity Biosciences Cat# DF9246, RRID:AB_2842442.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ABC13; ABCA5; ABCA5_HUMAN; ATP binding cassette sub family A (ABC1) member 5; ATP binding cassette A5; ATP binding cassette sub family A member 5; ATP-binding cassette sub-family A member 5; DKFZp451F117; DKFZp779N2435; EST90625; FLJ16381; KIAA1888;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q8WWZ7 ABCA5_HUMAN:

Ubiquitously expressed. Highly expressed in testis, skeletal muscle, kidney, liver and placenta.

Sequence:
MSTAIREVGVWRQTRTLLLKNYLIKCRTKKSSVQEILFPLFFLFWLILISMMHPNKKYEEVPNIELNPMDKFTLSNLILGYTPVTNITSSIMQKVSTDHLPDVIITEEYTNEKEMLTSSLSKPSNFVGVVFKDSMSYELRFFPDMIPVSSIYMDSRAGCSKSCEAAQYWSSGFTVLQASIDAAIIQLKTNVSLWKELESTKAVIMGETAVVEIDTFPRGVILIYLVIAFSPFGYFLAIHIVAEKEKKIKEFLKIMGLHDTAFWLSWVLLYTSLIFLMSLLMAVIATASLLFPQSSSIVIFLLFFLYGLSSVFFALMLTPLFKKSKHVGIVEFFVTVAFGFIGLMIILIESFPKSLVWLFSPFCHCTFVIGIAQVMHLEDFNEGASFSNLTAGPYPLIITIIMLTLNSIFYVLLAVYLDQVIPGEFGLRRSSLYFLKPSYWSKSKRNYEELSEGNVNGNISFSEIIEPVSSEFVGKEAIRISGIQKTYRKKGENVEALRNLSFDIYEGQITALLGHSGTGKSTLMNILCGLCPPSDGFASIYGHRVSEIDEMFEARKMIGICPQLDIHFDVLTVEENLSILASIKGIPANNIIQEVQKVLLDLDMQTIKDNQAKKLSGGQKRKLSLGIAVLGNPKILLLDEPTAGMDPCSRHIVWNLLKYRKANRVTVFSTHFMDEADILADRKAVISQGMLKCVGSSMFLKSKWGIGYRLSMYIDKYCATESLSSLVKQHIPGATLLQQNDQQLVYSLPFKDMDKFSGLFSALDSHSNLGVISYGVSMTTLEDVFLKLEVEAEIDQADYSVFTQQPLEEEMDSKSFDEMEQSLLILSETKAALVSTMSLWKQQMYTIAKFHFFTLKRESKSVRSVLLLLLIFFTVQIFMFLVHHSFKNAVVPIKLVPDLYFLKPGDKPHKYKTSLLLQNSADSDISDLISFFTSQNIMVTMINDSDYVSVAPHSAALNVMHSEKDYVFAAVFNSTMVYSLPILVNIISNYYLYHLNVTETIQIWSTPFFQEITDIVFKIELYFQAALLGIIVTAMPPYFAMENAENHKIKAYTQLKLSGLLPSAYWIGQAVVDIPLFFIILILMLGSLLAFHYGLYFYTVKFLAVVFCLIGYVPSVILFTYIASFTFKKILNTKEFWSFIYSVAALACIAITEITFFMGYTIATILHYAFCIIIPIYPLLGCLISFIKISWKNVRKNVDTYNPWDRLSVAVISPYLQCVLWIFLLQYYEKKYGGRSIRKDPFFRNLSTKSKNRKLPEPPDNEDEDEDVKAERLKVKELMGCQCCEEKPSIMVSNLHKEYDDKKDFLLSRKVKKVATKYISFCVKKGEILGLLGPNGAGKSTIINILVGDIEPTSGQVFLGDYSSETSEDDDSLKCMGYCPQINPLWPDTTLQEHFEIYGAVKGMSASDMKEVISRITHALDLKEHLQKTVKKLPAGIKRKLCFALSMLGNPQITLLDEPSTGMDPKAKQHMWRAIRTAFKNRKRAAILTTHYMEEAEAVCDRVAIMVSGQLRCIGTVQHLKSKFGKGYFLEIKLKDWIENLEVDRLQREIQYIFPNASRQESFSSILAYKIPKEDVQSLSQSFFKLEEAKHAFAIEEYSFSQATLEQVFVELTKEQEEEDNSCGTLNSTLWWERTQEDRVVF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
100
Pig
78
Bovine
67
Sheep
67
Zebrafish
63
Dog
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WWZ7 As Substrate

Site PTM Type Enzyme
S134 Phosphorylation
Y137 Phosphorylation
S155 Phosphorylation
S192 Phosphorylation
R218 Methylation
K490 Ubiquitination
K608 Ubiquitination
K614 Acetylation
S616 Phosphorylation
K620 Acetylation
S624 Phosphorylation
T642 Phosphorylation
S649 Phosphorylation
K683 Methylation
S687 Phosphorylation
K701 Ubiquitination
K703 Ubiquitination
T854 Phosphorylation
S859 Phosphorylation
K1050 Ubiquitination
K1254 Ubiquitination
Y1299 Phosphorylation
S1405 Phosphorylation
S1407 Phosphorylation
K1410 Ubiquitination
K1423 Ubiquitination
K1428 Ubiquitination
S1562 Phosphorylation
S1564 Phosphorylation
S1565 Phosphorylation
Y1569 Phosphorylation
K1585 Ubiquitination

Research Backgrounds

Function:

May play a role in the processing of autolysosomes.

PTMs:

N-glycosylated.

Subcellular Location:

Lysosome membrane>Multi-pass membrane protein. Late endosome membrane>Multi-pass membrane protein. Golgi apparatus membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed. Highly expressed in testis, skeletal muscle, kidney, liver and placenta.

Family&Domains:

Belongs to the ABC transporter superfamily. ABCA family.

Research Fields

· Environmental Information Processing > Membrane transport > ABC transporters.

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