ATP5L Antibody | Affinity Biosciences

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Western blot analysis of ATP5L using COLO205 whole cell lysates.

DF9241 at 1/100 staining Mouse colorectal tissue by IHC-P.

Product:	ATP5L Antibody
Catalog:	DF9241
Description:	Rabbit polyclonal antibody to ATP5L
Application:	WB IHC
Reactivity:	Human, Mouse
Prediction:	Zebrafish, Bovine
Mol.Wt.:	11kDa; 11kD(Calculated).
Uniprot:	O75964
RRID:	AB_2842437

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Zebrafish(92%), Bovine(100%)

Clonality:

Polyclonal

Specificity:

ATP5L Antibody detects endogenous levels of total ATP5L.

RRID:

AB_2842437
Cite Format: Affinity Biosciences Cat# DF9241, RRID:AB_2842437.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ATP synthase subunit g; ATP synthase, H+ transporting, mitochondrial F1F0, subunit g; ATP synthase, H+ transporting, mitochondrial Fo complex, subunit G; ATP5L; ATP5L_HUMAN; ATPase subunit g; F1Fo ATP synthase complex Fo membrane domain g subunit; mitochondrial;

Immunogens

Immunogen:

Uniprot:

O75964(ATP5L_HUMAN) >>Visit HPA database.

Gene(ID):

ATP5L(10632)

Sequence:

O75964 ATP5L_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAQFVRNLVEKTPALVNAAVTYSKPRLATFWYYAKVELVPPTPAEIPRAIQSLKKIVNSAQTGSFKQLTVKEAVLNGLVATEVLMWFYVGEIIGKRGIIGYDV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Zebrafish

Pig

Horse

Sheep

Dog

Xenopus

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75964 As Substrate

O75964

Site	PTM Type	Source
A2	Acetylation	Uniprot
K11	Ubiquitination	Uniprot
K24	Acetylation	Uniprot
K24	Ubiquitination	Uniprot
T29	Phosphorylation	Uniprot
Y32	Phosphorylation	Uniprot
T42	Phosphorylation	Uniprot
K54	Acetylation	Uniprot
K54	Ubiquitination	Uniprot
K55	Ubiquitination	Uniprot
S59	Phosphorylation	Uniprot
T62	Phosphorylation	Uniprot
S64	Phosphorylation	Uniprot
K66	Acetylation	Uniprot
K66	Methylation	Uniprot
K66	Ubiquitination	Uniprot

Research Backgrounds

O75964_ATP5L_HUMAN

Function:

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.

Subcellular Location:

Mitochondrion. Mitochondrion inner membrane.

Subunit Structure:

F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(0) seems to have nine subunits: a, b, c, d, e, f, g, F6 and 8 (or A6L). Component of an ATP synthase complex composed of ATP5PB, ATP5MC1, ATP5F1E, ATP5PD, ATP5ME, ATP5PF, ATP5MF, MT-ATP6, MT-ATP8, ATP5F1A, ATP5F1B, ATP5F1D, ATP5F1C, ATP5PO, ATP5MG, ATP5MD and ATP5MPL (By similarity).

Family&Domains:

Belongs to the ATPase g subunit family.

Research Fields

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

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ATP5L Antibody - #DF9241