MT-ATP8 Antibody | Affinity Biosciences

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Western blot analysis of MT-ATP8 using Jurkat whole cell lysates.

Product:	MT-ATP8 Antibody
Catalog:	DF9240
Description:	Rabbit polyclonal antibody to MT-ATP8
Application:	WB
Reactivity:	Human
Mol.Wt.:	8 kDa; 8kD(Calculated).
Uniprot:	P03928
RRID:	AB_2842436

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

MT-ATP8 Antibody detects endogenous levels of total MT-ATP8.

RRID:

AB_2842436
Cite Format: Affinity Biosciences Cat# DF9240, RRID:AB_2842436.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:

Uniprot:

P03928(ATP8_HUMAN) >>Visit HPA database.

Gene(ID):

MT-ATP8(4509)

Sequence:

P03928 ATP8_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPQLNTTVWPTMITPMLLTLFLITQLKMLNTNYHLPPSPKPMKMKNYNKPWEPKWTKICSLHSLPPQS

PTMs - P03928 As Substrate

P03928

Site	PTM Type	Source
T31	Phosphorylation	Uniprot
Y33	Phosphorylation	Uniprot
S38	Phosphorylation	Uniprot
K40	Ubiquitination	Uniprot
Y47	Phosphorylation	Uniprot
K49	Acetylation	Uniprot
K54	Ubiquitination	Uniprot
K57	Ubiquitination	Uniprot

Research Backgrounds

P03928_ATP8_HUMAN

Function:

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane (By similarity).

Subcellular Location:

Mitochondrion membrane>Single-pass membrane protein.

Subunit Structure:

F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. Component of an ATP synthase complex composed of ATP5PB, ATP5MC1, ATP5F1E, ATP5PD, ATP5ME, ATP5PF, ATP5MF, MT-ATP6, MT-ATP8, ATP5F1A, ATP5F1B, ATP5F1D, ATP5F1C, ATP5PO, ATP5MG, ATP5MD and ATP5MPL (By similarity).

Family&Domains:

Belongs to the ATPase protein 8 family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

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For Research Use Only.
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MT-ATP8 Antibody - #DF9240