Cytokeratin 20 Antibody - #AF0193
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF0193, RRID:AB_2833386.
Fold/Unfold
CD20; CK 20; CK-20; CK20; Cytokeratin-20; Cytokeratin20; K1C20_HUMAN; K20; KA20; Keratin 20; keratin 20, type I; keratin 21, rat, homolog of; Keratin; Keratin type I cytoskeletal 20; Keratin-20; Keratin20; KRT 20; KRT 21; KRT20; KRT21; MGC35423; OTTHUMP00000164518; Protein IT; type I cytoskeletal 20;
Immunogens
Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
- P35900 K1C20_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDFSRRSFHRSLSSSLQAPVVSTVGMQRLGTTPSVYGGAGGRGIRISNSRHTVNYGSDLTGGGDLFVGNEKMAMQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAYYRQIEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADLQGLNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTVNVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELKGTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLEETKARYSSQLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P35900 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S11 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | P49137 (MAPKAPK2) , P17252 (PRKCA) | Uniprot |
| S34 | Phosphorylation | Uniprot | |
| Y36 | Phosphorylation | Uniprot | |
| R42 | Methylation | Uniprot | |
| Y84 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| S118 | Phosphorylation | Uniprot | |
| Y120 | Phosphorylation | Uniprot | |
| Y121 | Phosphorylation | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| T168 | Phosphorylation | Uniprot | |
| T369 | Phosphorylation | Uniprot | |
| Y370 | Phosphorylation | Uniprot | |
| Y384 | Phosphorylation | Uniprot | |
| S387 | Phosphorylation | Uniprot | |
| T402 | Phosphorylation | Uniprot |
Research Backgrounds
Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).
Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity).
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
Cytoplasm.
Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
Heterotetramer of two type I and two type II keratins. Associates with KRT8.
Belongs to the intermediate filament family.
Research Fields
· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)
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