Product: AE2 Antibody
Catalog: DF9212
Description: Rabbit polyclonal antibody to AE2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Dog
Mol.Wt.: 137 kDa; 137kD(Calculated).
Uniprot: P04920
RRID: AB_2842408

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(89%), Bovine(88%), Sheep(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
AE2 Antibody detects endogenous levels of total AE2.
RRID:
AB_2842408
Cite Format: Affinity Biosciences Cat# DF9212, RRID:AB_2842408.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AE 2; AE2 anion exchanger; Anion exchange protein 2; Anion exchanger 2; B3A2_HUMAN; BAND 3-LIKE PROTEIN; BND3L; EPB3L1; erythrocyte band 3; Erythrocyte membrane protein band 3 like 1; HKB3; MPB3L; NBND3; Non-erythroid band 3-like protein; Slc4a2; Solute carrier family 4 member 2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MSSAPRRPAKGADSFCTPEPESLGPGTPGFPEQEEDELHRTLGVERFEEILQEAGSRGGEEPGRSYGEEDFEYHRQSSHHIHHPLSTHLPPDARRRKTPQGPGRKPRRRPGASPTGETPTIEEGEEDEDEASEAEGARALTQPSPVSTPSSVQFFLQEDDSADRKAERTSPSSPAPLPHQEATPRASKGAQAGTQVEEAEAEAVAVASGTAGGDDGGASGRPLPKAQPGHRSYNLQERRRIGSMTGAEQALLPRVPTDEIEAQTLATADLDLMKSHRFEDVPGVRRHLVRKNAKGSTQSGREGREPGPTPRARPRAPHKPHEVFVELNELLLDKNQEPQWRETARWIKFEEDVEEETERWGKPHVASLSFRSLLELRRTLAHGAVLLDLDQQTLPGVAHQVVEQMVISDQIKAEDRANVLRALLLKHSHPSDEKDFSFPRNISAGSLGSLLGHHHGQGAESDPHVTEPLMGGVPETRLEVERERELPPPAPPAGITRSKSKHELKLLEKIPENAEATVVLVGCVEFLSRPTMAFVRLREAVELDAVLEVPVPVRFLFLLLGPSSANMDYHEIGRSISTLMSDKQFHEAAYLADEREDLLTAINAFLDCSVVLPPSEVQGEELLRSVAHFQRQMLKKREEQGRLLPTGAGLEPKSAQDKALLQMVEAAGAAEDDPLRRTGRPFGGLIRDVRRRYPHYLSDFRDALDPQCLAAVIFIYFAALSPAITFGGLLGEKTQDLIGVSELIMSTALQGVVFCLLGAQPLLVIGFSGPLLVFEEAFFSFCSSNHLEYLVGRVWIGFWLVFLALLMVALEGSFLVRFVSRFTQEIFAFLISLIFIYETFYKLVKIFQEHPLHGCSASNSSEVDGGENMTWAGARPTLGPGNRSLAGQSGQGKPRGQPNTALLSLVLMAGTFFIAFFLRKFKNSRFFPGRIRRVIGDFGVPIAILIMVLVDYSIEDTYTQKLSVPSGFSVTAPEKRGWVINPLGEKSPFPVWMMVASLLPAILVFILIFMETQITTLIISKKERMLQKGSGFHLDLLLIVAMGGICALFGLPWLAAATVRSVTHANALTVMSKAVAPGDKPKIQEVKEQRVTGLLVALLVGLSIVIGDLLRQIPLAVLFGIFLYMGVTSLNGIQFYERLHLLLMPPKHHPDVTYVKKVRTLRMHLFTALQLLCLALLWAVMSTAASLAFPFILILTVPLRMVVLTRIFTDREMKCLDANEAEPVFDEREGVDEYNEMPMPV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
89
Bovine
88
Sheep
88
Dog
88
Horse
78
Rabbit
78
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P04920 As Substrate

Site PTM Type Enzyme
T27 Phosphorylation
S56 Phosphorylation
R57 Methylation
S65 Phosphorylation
Y66 Phosphorylation
Y73 Phosphorylation
S113 Phosphorylation
T115 Phosphorylation
T118 Phosphorylation
S132 Phosphorylation
S144 Phosphorylation
T148 Phosphorylation
S150 Phosphorylation
S151 Phosphorylation
T169 Phosphorylation
S170 Phosphorylation
S172 Phosphorylation
S173 Phosphorylation
T183 Phosphorylation
S232 Phosphorylation
Y233 Phosphorylation
S243 Phosphorylation
T245 Phosphorylation
T257 Phosphorylation
T264 Phosphorylation
K274 Methylation
T309 Phosphorylation
K319 Ubiquitination
K334 Ubiquitination
K362 Ubiquitination
K426 Ubiquitination
K434 Ubiquitination
S443 Phosphorylation
S446 Phosphorylation
S449 Phosphorylation
S461 Phosphorylation
S500 Phosphorylation
K505 Ubiquitination
K636 Ubiquitination
K653 Ubiquitination
K658 Ubiquitination
K893 Ubiquitination
K975 Ubiquitination
K1073 Ubiquitination
K1080 Ubiquitination
K1087 Ubiquitination
Y1154 Phosphorylation
K1156 Ubiquitination
Y1234 Phosphorylation

Research Backgrounds

Function:

Plasma membrane anion exchange protein of wide distribution.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the anion exchanger (TC 2.A.31) family.

Research Fields

· Organismal Systems > Digestive system > Salivary secretion.

· Organismal Systems > Digestive system > Gastric acid secretion.

· Organismal Systems > Digestive system > Pancreatic secretion.

References

1). Knockdown of anion exchanger 2 suppressed the growth of ovarian cancer cells via mTOR/p70S6K1 signaling. Scientific Reports, 2017 (PubMed: 28743911) [IF=4.6]

Application: WB    Species: Human    Sample: ovarian cancer tissues

Figure 1 AE2 was up-regulated in human ovarian cancer tissues. (A) AE2 mRNA was over-expressed in ovarian cancer tissues compared to the normal tissues in TCGA dataset. Box-whiskers plot (Min to Max) was shown. (B) Genomic alterations of AE1 (SLC4A1), AE2 (SLC4A2) and AE3 (SLC4A3) in cBioportal ovarian cancer database. (C) mRNA expression of AE2 was examined by qPCR and normalized to GAPDH expression in 100 pairs of matched ovarian cancer tissues and adjacent non-tumorous tissue. Box-whiskers plot (Min to Max) was shown. (D) The protein levels of AE2 were higher in 10 pairs of matched samples of ovarian cancer tissue (T), compared with adjacent non-tumorous tissues (N). GAPDH was served as a loading control. (E) Kaplan–Meier survival analysis of 100 patients with ovarian cancer.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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