AE2 Antibody - #DF9212
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Product Info
Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(89%), Bovine(88%), Sheep(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
AE2 Antibody detects endogenous levels of total AE2.
RRID:
AB_2842408
Cite Format: Affinity Biosciences Cat# DF9212, RRID:AB_2842408.
Cite Format: Affinity Biosciences Cat# DF9212, RRID:AB_2842408.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
AE 2; AE2 anion exchanger; Anion exchange protein 2; Anion exchanger 2; B3A2_HUMAN; BAND 3-LIKE PROTEIN; BND3L; EPB3L1; erythrocyte band 3; Erythrocyte membrane protein band 3 like 1; HKB3; MPB3L; NBND3; Non-erythroid band 3-like protein; Slc4a2; Solute carrier family 4 member 2;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- P04920 B3A2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSAPRRPAKGADSFCTPEPESLGPGTPGFPEQEEDELHRTLGVERFEEILQEAGSRGGEEPGRSYGEEDFEYHRQSSHHIHHPLSTHLPPDARRRKTPQGPGRKPRRRPGASPTGETPTIEEGEEDEDEASEAEGARALTQPSPVSTPSSVQFFLQEDDSADRKAERTSPSSPAPLPHQEATPRASKGAQAGTQVEEAEAEAVAVASGTAGGDDGGASGRPLPKAQPGHRSYNLQERRRIGSMTGAEQALLPRVPTDEIEAQTLATADLDLMKSHRFEDVPGVRRHLVRKNAKGSTQSGREGREPGPTPRARPRAPHKPHEVFVELNELLLDKNQEPQWRETARWIKFEEDVEEETERWGKPHVASLSFRSLLELRRTLAHGAVLLDLDQQTLPGVAHQVVEQMVISDQIKAEDRANVLRALLLKHSHPSDEKDFSFPRNISAGSLGSLLGHHHGQGAESDPHVTEPLMGGVPETRLEVERERELPPPAPPAGITRSKSKHELKLLEKIPENAEATVVLVGCVEFLSRPTMAFVRLREAVELDAVLEVPVPVRFLFLLLGPSSANMDYHEIGRSISTLMSDKQFHEAAYLADEREDLLTAINAFLDCSVVLPPSEVQGEELLRSVAHFQRQMLKKREEQGRLLPTGAGLEPKSAQDKALLQMVEAAGAAEDDPLRRTGRPFGGLIRDVRRRYPHYLSDFRDALDPQCLAAVIFIYFAALSPAITFGGLLGEKTQDLIGVSELIMSTALQGVVFCLLGAQPLLVIGFSGPLLVFEEAFFSFCSSNHLEYLVGRVWIGFWLVFLALLMVALEGSFLVRFVSRFTQEIFAFLISLIFIYETFYKLVKIFQEHPLHGCSASNSSEVDGGENMTWAGARPTLGPGNRSLAGQSGQGKPRGQPNTALLSLVLMAGTFFIAFFLRKFKNSRFFPGRIRRVIGDFGVPIAILIMVLVDYSIEDTYTQKLSVPSGFSVTAPEKRGWVINPLGEKSPFPVWMMVASLLPAILVFILIFMETQITTLIISKKERMLQKGSGFHLDLLLIVAMGGICALFGLPWLAAATVRSVTHANALTVMSKAVAPGDKPKIQEVKEQRVTGLLVALLVGLSIVIGDLLRQIPLAVLFGIFLYMGVTSLNGIQFYERLHLLLMPPKHHPDVTYVKKVRTLRMHLFTALQLLCLALLWAVMSTAASLAFPFILILTVPLRMVVLTRIFTDREMKCLDANEAEPVFDEREGVDEYNEMPMPV
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
89
Bovine
88
Sheep
88
Dog
88
Horse
78
Rabbit
78
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P04920 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T27 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| R57 | Methylation | Uniprot | |
| S65 | Phosphorylation | Uniprot | |
| Y66 | Phosphorylation | Uniprot | |
| Y73 | Phosphorylation | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| T115 | Phosphorylation | Uniprot | |
| T118 | Phosphorylation | Uniprot | |
| S132 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| T148 | Phosphorylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| T169 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| T183 | Phosphorylation | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| Y233 | Phosphorylation | Uniprot | |
| S243 | Phosphorylation | Uniprot | |
| T245 | Phosphorylation | Uniprot | |
| T257 | Phosphorylation | Uniprot | |
| T264 | Phosphorylation | Uniprot | |
| K274 | Methylation | Uniprot | |
| T309 | Phosphorylation | Uniprot | |
| K319 | Ubiquitination | Uniprot | |
| K334 | Ubiquitination | Uniprot | |
| K362 | Ubiquitination | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| K434 | Ubiquitination | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| S446 | Phosphorylation | Uniprot | |
| S449 | Phosphorylation | Uniprot | |
| S461 | Phosphorylation | Uniprot | |
| S500 | Phosphorylation | Uniprot | |
| K505 | Ubiquitination | Uniprot | |
| K636 | Ubiquitination | Uniprot | |
| K653 | Ubiquitination | Uniprot | |
| K658 | Ubiquitination | Uniprot | |
| K893 | Ubiquitination | Uniprot | |
| K975 | Ubiquitination | Uniprot | |
| K1073 | Ubiquitination | Uniprot | |
| K1080 | Ubiquitination | Uniprot | |
| K1087 | Ubiquitination | Uniprot | |
| Y1154 | Phosphorylation | Uniprot | |
| K1156 | Ubiquitination | Uniprot | |
| Y1234 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Plasma membrane anion exchange protein of wide distribution.
Subcellular Location:
Membrane>Multi-pass membrane protein.
Family&Domains:
Belongs to the anion exchanger (TC 2.A.31) family.
Research Fields
· Organismal Systems > Digestive system > Salivary secretion.
· Organismal Systems > Digestive system > Gastric acid secretion.
· Organismal Systems > Digestive system > Pancreatic secretion.
References
1). Knockdown of anion exchanger 2 suppressed the growth of ovarian cancer cells via mTOR/p70S6K1 signaling. Scientific Reports, 2017
(PubMed: 28743911)
[IF=4.6]
Application: WB Species: Human Sample: ovarian cancer tissues
Figure 1
AE2 was up-regulated in human ovarian cancer tissues. (A) AE2 mRNA was over-expressed in ovarian cancer tissues compared to the normal tissues in TCGA dataset. Box-whiskers plot (Min to Max) was shown. (B) Genomic alterations of AE1 (SLC4A1), AE2 (SLC4A2) and AE3 (SLC4A3) in cBioportal ovarian cancer database. (C) mRNA expression of AE2 was examined by qPCR and normalized to GAPDH expression in 100 pairs of matched ovarian cancer tissues and adjacent non-tumorous tissue. Box-whiskers plot (Min to Max) was shown. (D) The protein levels of AE2 were higher in 10 pairs of matched samples of ovarian cancer tissue (T), compared with adjacent non-tumorous tissues (N). GAPDH was served as a loading control. (E) Kaplan–Meier survival analysis of 100 patients with ovarian cancer.
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