AMPD3 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of AMPD3 using MCF7 whole cell lysates.

DF9200 at 1/100 staining rat testis tissue by IHC-P.

Product:	AMPD3 Antibody
Catalog:	DF9200
Description:	Rabbit polyclonal antibody to AMPD3
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Sheep, Xenopus
Mol.Wt.:	89 kDa; 89kD(Calculated).
Uniprot:	Q01432
RRID:	AB_2842396

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(88%), Sheep(88%), Xenopus(88%)

Clonality:

Polyclonal

Specificity:

AMPD3 Antibody detects endogenous levels of total AMPD3.

RRID:

AB_2842396
Cite Format: Affinity Biosciences Cat# DF9200, RRID:AB_2842396.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Adenosine monophosphate deaminase (isoform E); Adenosine monophosphate deaminase 3; AMP aminohydrolase; AMP deaminase 3; AMP deaminase isoform E; Ampd3; AMPD3_HUMAN; Erythrocyte AMP deaminase; Erythrocyte specific AMP deaminase; Erythrocyte specific protein; Erythrocyte type AMP deaminase; Erythroid Marker; Myoadenylate deaminase; OTTHUMP00000230743; OTTHUMP00000230746; OTTHUMP00000230747; OTTHUMP00000230748; OTTHUMP00000230749;

Immunogens

Immunogen:

Uniprot:

Q01432(AMPD3_HUMAN) >>Visit HPA database.

Gene(ID):

AMPD3(272)

Sequence:

Q01432 AMPD3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPRQFPKLNISEVDEQVRLLAEKVFAKVLREEDSKDALSLFTVPEDCPIGQKEAKERELQKELAEQKSVETAKRKKSFKMIRSQSLSLQMPPQQDWKGPPAASPAMSPTTPVVTGATSLPTPAPYAMPEFQRVTISGDYCAGITLEDYEQAAKSLAKALMIREKYARLAYHRFPRITSQYLGHPRADTAPPEEGLPDFHPPPLPQEDPYCLDDAPPNLDYLVHMQGGILFVYDNKKMLEHQEPHSLPYPDLETYTVDMSHILALITDGPTKTYCHRRLNFLESKFSLHEMLNEMSEFKELKSNPHRDFYNVRKVDTHIHAAACMNQKHLLRFIKHTYQTEPDRTVAEKRGRKITLRQVFDGLHMDPYDLTVDSLDVHAGRQTFHRFDKFNSKYNPVGASELRDLYLKTENYLGGEYFARMVKEVARELEESKYQYSEPRLSIYGRSPEEWPNLAYWFIQHKVYSPNMRWIIQVPRIYDIFRSKKLLPNFGKMLENIFLPLFKATINPQDHRELHLFLKYVTGFDSVDDESKHSDHMFSDKSPNPDVWTSEQNPPYSYYLYYMYANIMVLNNLRRERGLSTFLFRPHCGEAGSITHLVSAFLTADNISHGLLLKKSPVLQYLYYLAQIPIAMSPLSNNSLFLEYSKNPLREFLHKGLHVSLSTDDPMQFHYTKEALMEEYAIAAQVWKLSTCDLCEIARNSVLQSGLSHQEKQKFLGQNYYKEGPEGNDIRKTNVAQIRMAFRYETLCNELSFLSDAMKSEEITALTN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Sheep

Xenopus

Pig

Horse

Dog

Rabbit

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q01432 As Substrate

Q01432

Site	PTM Type	Source
K7	Ubiquitination	Uniprot
K23	Ubiquitination	Uniprot
K27	Methylation	Uniprot
K27	Ubiquitination	Uniprot
K35	Ubiquitination	Uniprot
K61	Ubiquitination	Uniprot
K67	Ubiquitination	Uniprot
S83	Phosphorylation	Uniprot
S85	Phosphorylation	Uniprot
S87	Phosphorylation	Uniprot
S103	Phosphorylation	Uniprot
S107	Phosphorylation	Uniprot
K157	Ubiquitination	Uniprot
Y170	Phosphorylation	Uniprot
S178	Phosphorylation	Uniprot
K298	Ubiquitination	Uniprot
K301	Ubiquitination	Uniprot
K348	Ubiquitination	Uniprot
K388	Ubiquitination	Uniprot
K407	Ubiquitination	Uniprot
K422	Ubiquitination	Uniprot
S431	Phosphorylation	Uniprot
K432	Ubiquitination	Uniprot
Y433	Phosphorylation	Uniprot
Y435	Phosphorylation	Uniprot
T521	Phosphorylation	Uniprot
S525	Phosphorylation	Uniprot
S530	Phosphorylation	Uniprot
S538	Phosphorylation	Uniprot
K711	Ubiquitination	Uniprot
K721	Ubiquitination	Uniprot
K731	Ubiquitination	Uniprot
T732	Phosphorylation	Uniprot

Research Backgrounds

Q01432_AMPD3_HUMAN

Function:

AMP deaminase plays a critical role in energy metabolism.

Subunit Structure:

Homotetramer.

Family&Domains:

Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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For Research Use Only.
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AMPD3 Antibody - #DF9200