Product: AKR1C4 Antibody
Catalog: DF9190
Description: Rabbit polyclonal antibody to AKR1C4
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Mol.Wt.: 37 kDa; 37kD(Calculated).
Uniprot: P17516
RRID: AB_2842386

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Clonality:
Polyclonal
Specificity:
AKR1C4 Antibody detects endogenous levels of total AKR1C4.
RRID:
AB_2842386
Cite Format: Affinity Biosciences Cat# DF9190, RRID:AB_2842386.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

3 alpha HSD1; 3-alpha-HSD1; 3-alpha-hydroxysteroid dehydrogenase type I; AK1C4_HUMAN; AKR1C4; Aldo keto reductase family 1 member C4; Aldo-keto reductase family 1 member C4; CDR; Chlordecone reductase; DD-4; DD4; Dihydrodiol dehydrogenase 4; HAKRA;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P17516 AK1C4_HUMAN:

Liver specific.

Sequence:
MDPKYQRVELNDGHFMPVLGFGTYAPPEVPRNRAVEVTKLAIEAGFRHIDSAYLYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWCTFFQPQMVQPALESSLKKLQLDYVDLYLLHFPMALKPGETPLPKDENGKVIFDTVDLSATWEVMEKCKDAGLAKSIGVSNFNCRQLEMILNKPGLKYKPVCNQVECHPYLNQSKLLDFCKSKDIVLVAHSALGTQRHKLWVDPNSPVLLEDPVLCALAKKHKQTPALIALRYQLQRGVVVLAKSYNEQRIRENIQVFEFQLTSEDMKVLDGLNRNYRYVVMDFLMDHPDYPFSDEY

PTMs - P17516 As Substrate

Site PTM Type Enzyme
K4 Acetylation
T23 Phosphorylation
Y24 Phosphorylation
Y53 Phosphorylation
K68 Ubiquitination
S73 Phosphorylation
K75 Acetylation
K75 Sumoylation
K75 Ubiquitination
Y81 Phosphorylation
T82 Phosphorylation
S83 Phosphorylation
K161 Ubiquitination
K179 Ubiquitination
K183 Ubiquitination
Y184 Phosphorylation
Y196 Phosphorylation
S200 Phosphorylation
K201 Acetylation
K201 Ubiquitination
K207 Ubiquitination
S208 Phosphorylation
K209 Ubiquitination
S232 Phosphorylation
K246 Acetylation
K246 Ubiquitination
K247 Acetylation
K247 Ubiquitination
K270 Acetylation
K270 Ubiquitination
Y272 Phosphorylation
S290 Phosphorylation
Y305 Phosphorylation
Y317 Phosphorylation

Research Backgrounds

Function:

Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol). Also has some 20-alpha-hydroxysteroid dehydrogenase activity. The biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leads to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route.

PTMs:

The N-terminus is blocked.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Liver specific.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the aldo/keto reductase family.

Research Fields

· Metabolism > Lipid metabolism > Primary bile acid biosynthesis.

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Prognostic significance of AKR1C4 and the advantage of combining EBV DNA to stratify patients at high risk of locoregional recurrence of nasopharyngeal carcinoma. BMC Cancer, 2022 (PubMed: 35953777) [IF=3.8]

Application: WB    Species: Human    Sample: NPC cell

Fig. 1 AKR1C4 expression profile. A Heat map demonstrated expression pattern of different genes in HONE1 vs. HONE1-IR. High or low expression was reflected as red or blue indicated in the scale bar, respectively. Herein, locus AK314988 (highlighted by red frame), which was AKR1C4, showed a significant higher expression in HONE1-IR than in HONE1. B In scatter plot, red and blue dots represented upregulation and downregulation of gene expression, respectively, and AKR1C4 expression was elevated in HONE1-IR cell line, which indicated a robust replicability of RNA-seq samples. C Representative images of different intensities of immunohistochemical staining for AKR1C4 in nasopharyngeal carcinoma (NPC) tissues (200 ×): Negative staining, weak staining, moderate staining, and strong staining. All micrographs were taken and processed at identical conditions. Scale bar: 50 μm. D IHC result of normal nasopharyngeal mucosa stained for AKR1C4, in magnification 40 × (left) and 200 × (right). E Protein levels of AKR1C4 in different NPC cell lines (HNE1, 6-10B, CNE2, S18, S26, CNE1, 5-8F and SUNE2) and normal nasopharyngeal epithelial cell line NP69 were evaluated by western blotting. β-tubulin was used as internal control

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