GPT2 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from HepG2 cells, using GPT2 Antibody.

Product:	GPT2 Antibody
Catalog:	DF9183
Description:	Rabbit polyclonal antibody to GPT2
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Bovine, Horse, Sheep, Dog
Mol.Wt.:	58 kDa; 58kD(Calculated).
Uniprot:	Q8TD30
RRID:	AB_2842379

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Bovine(89%), Horse(89%), Sheep(89%), Dog(100%)

Clonality:

Polyclonal

Specificity:

GPT2 Antibody detects endogenous levels of total GPT2.

RRID:

AB_2842379
Cite Format: Affinity Biosciences Cat# DF9183, RRID:AB_2842379.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AAT2; Alanine aminotransferase 2; ALAT2_HUMAN; ALT2; Glutamate pyruvate transaminase 2; Glutamic alanine transaminase 2; Glutamic pyruvate transaminase (alanine aminotransferase) 2; Glutamic pyruvic transaminase 2; Glutamic--alanine transaminase 2; Glutamic--pyruvic transaminase 2; GPT 2; gpt2;

Immunogens

Immunogen:

Uniprot:

Q8TD30(ALAT2_HUMAN) >>Visit HPA database.

Gene(ID):

GPT2(84706)

Expression:

Q8TD30 ALAT2_HUMAN:

Expressed at high levels in muscle, adipose tissue, kidney and brain and at lower levels in the liver and breast.

Sequence:

Q8TD30 ALAT2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MQRAAALVRRGCGPRTPSSWGRSQSSAAAEASAVLKVRPERSRRERILTLESMNPQVKAVEYAVRGPIVLKAGEIELELQRGIKKPFTEVIRANIGDAQAMGQQPITFLRQVMALCTYPNLLDSPSFPEDAKKRARRILQACGGNSLGSYSASQGVNCIREDVAAYITRRDGGVPADPDNIYLTTGASDGISTILKILVSGGGKSRTGVMIPIPQYPLYSAVISELDAIQVNYYLDEENCWALNVNELRRAVQEAKDHCDPKVLCIINPGNPTGQVQSRKCIEDVIHFAWEEKLFLLADEVYQDNVYSPDCRFHSFKKVLYEMGPEYSSNVELASFHSTSKGYMGECGYRGGYMEVINLHPEIKGQLVKLLSVRLCPPVSGQAAMDIVVNPPVAGEESFEQFSREKESVLGNLAKKAKLTEDLFNQVPGIHCNPLQGAMYAFPRIFIPAKAVEAAQAHQMAPDMFYCMKLLEETGICVVPGSGFGQREGTYHFRMTILPPVEKLKTVLQKVKDFHINFLEKYA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

100

Horse

Bovine

Sheep

Pig

Rabbit

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8TD30 As Substrate

Q8TD30

Site	PTM Type	Source
S25	Phosphorylation	Uniprot
R65	Methylation	Uniprot
T88	Phosphorylation	Uniprot
K406	Ubiquitination	Uniprot
Y491	Phosphorylation	Uniprot

Research Backgrounds

Q8TD30_ALAT2_HUMAN

Function:

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate.

Tissue Specificity:

Expressed at high levels in muscle, adipose tissue, kidney and brain and at lower levels in the liver and breast.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Research Fields

· Metabolism > Amino acid metabolism > Arginine biosynthesis.

· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > 2-Oxocarboxylic acid metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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GPT2 Antibody - #DF9183