AFF4 Antibody - #DF9180

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Product: AFF4 Antibody
Catalog: DF9180
Description: Rabbit polyclonal antibody to AFF4
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Chicken
Mol.Wt.: 127kDa; 127kD(Calculated).
Uniprot: Q9UHB7
RRID: AB_2842376

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
AFF4 Antibody detects endogenous levels of total AFF4.
RRID:
AB_2842376
Cite Format: Affinity Biosciences Cat# DF9180, RRID:AB_2842376.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AF4/FMR2 family member 4; AF5Q31; Alf4; ALL1 fused gene from chromosome 5q31; HSPC092; Laf4; Lymphoid nuclear protein related to AF4 like; Major CDK9 elongation factor associated protein; MCEF; MGC107469; RP23-226J16.7;

Immunogens

Immunogen:
Uniprot:

Q9UHB7(AFF4_HUMAN) >>Visit HPA database.

Gene(ID):
AFF4(27125)
Expression:
Q9UHB7 AFF4_HUMAN:

Ubiquitously expressed. Strongly expressed in heart, placenta, skeletal muscle, pancreas and to a lower extent in brain.

Sequence:
MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPSADEKSNPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRTSAGSSSGTNSSGQRHDRESYNNSGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLNSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRVPFSSGQHSTQSFPPSLMSKSNSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQSNFGTGEQKRYNPSKTSNGHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGREQGTGNSYTDTSGPKETSSATPGRDSKTIQKGSESGRGRQKSPAQSDSTTQRRTVGKKQPKKAEKAAAEEPRGGLKIESETPVDLASSMPSSRHKAATKGSRKPNIKKESKSSPRPTAEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHTREAQKQASEKVSNKGKRKHKNEDDNRASESKKPKTEDKNSAGHKPSSNRESSKQSAAKEKDLLPSPAGPVPSKDPKTEHGSRKRTISQSSSLKSSSNSNKETSGSSKNSSSTSKQKKTEGKTSSSSKEVKEKAPSSSSNCPPSAPTLDSSKPRRTKLVFDDRNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFPMYSETVDLIKYTMKLKNYLAPDATAADKRLTVLCLRCESLLYLRLFKLKKENALKYSKTLTEHLKNSYNNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGNYSSGASSASASGSSVTIPQKIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLVRYTRQGLHWLRQDAKLIS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Chicken
100
Dog
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UHB7 As Substrate

Site PTM Type Enzyme
S31 Phosphorylation
S32 Phosphorylation
Y39 Phosphorylation
S49 Phosphorylation
K79 Acetylation
S90 Phosphorylation
K107 Ubiquitination
T109 Phosphorylation
T117 Phosphorylation
S120 Phosphorylation
S124 Phosphorylation
S132 Phosphorylation
S133 Phosphorylation
T136 Phosphorylation
S155 Phosphorylation
Y156 Phosphorylation
S161 Phosphorylation
S163 Phosphorylation
K166 Acetylation
S176 Phosphorylation
S178 Phosphorylation
S179 Phosphorylation
S180 Phosphorylation
S188 Phosphorylation
S193 Phosphorylation
S195 Phosphorylation
S210 Phosphorylation
S212 Phosphorylation
S222 Phosphorylation
S224 Phosphorylation
K252 Acetylation
K252 Ubiquitination
S265 Phosphorylation
K288 Ubiquitination
K292 Ubiquitination
K297 Ubiquitination
K299 Ubiquitination
S302 Phosphorylation
S308 Phosphorylation
S310 Phosphorylation
S314 Phosphorylation
K321 Ubiquitination
T332 Phosphorylation
T336 Phosphorylation
K344 Ubiquitination
K350 Ubiquitination
T359 Phosphorylation
K363 Ubiquitination
S371 Phosphorylation
S387 Phosphorylation
S388 Phosphorylation
S389 Phosphorylation
S392 Phosphorylation
S440 Phosphorylation
S487 Phosphorylation
S490 Phosphorylation
S491 Phosphorylation
S494 Phosphorylation
S498 Phosphorylation
S499 Phosphorylation
Y502 Phosphorylation
K504 Acetylation
T511 Phosphorylation
S514 Phosphorylation
T516 Phosphorylation
T518 Phosphorylation
S519 Phosphorylation
S526 Phosphorylation
T528 Phosphorylation
S533 Phosphorylation
S549 Phosphorylation
S553 Phosphorylation
K583 Sumoylation
T588 Phosphorylation
S599 Phosphorylation
S619 Phosphorylation
S620 Phosphorylation
Y668 Phosphorylation
S671 Phosphorylation
T674 Phosphorylation
K677 Ubiquitination
S679 Phosphorylation
S680 Phosphorylation
S686 Phosphorylation
S694 Phosphorylation
S703 Phosphorylation
S706 Phosphorylation
Y712 Phosphorylation
K747 Acetylation
S789 Phosphorylation
S800 Phosphorylation
S801 Phosphorylation
S804 Phosphorylation
S814 Phosphorylation
S821 Phosphorylation
K822 Acetylation
T834 Phosphorylation
S836 Phosphorylation
S839 Phosphorylation
S840 Phosphorylation
S845 Phosphorylation
S847 Phosphorylation
K849 Acetylation
T867 Phosphorylation
T871 Phosphorylation
S872 Phosphorylation
S873 Phosphorylation
S874 Phosphorylation
S875 Phosphorylation
K905 Ubiquitination
R911 Methylation
R935 Methylation
S963 Phosphorylation
S965 Phosphorylation
T973 Phosphorylation
Y986 Phosphorylation
T999 Phosphorylation
K1026 Ubiquitination
S1039 Phosphorylation
S1043 Phosphorylation
S1048 Phosphorylation
S1055 Phosphorylation
S1058 Phosphorylation
S1062 Phosphorylation
S1066 Phosphorylation
Y1069 Phosphorylation
K1121 Ubiquitination

Research Backgrounds

Function:

Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression.

Subcellular Location:

Nucleus.
Note: Associates to transcriptionally active chromatin but not at snRNA genes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed. Strongly expressed in heart, placenta, skeletal muscle, pancreas and to a lower extent in brain.

Subunit Structure:

Interacts with ELL3; the interaction is direct (By similarity). Interacts with ELL2; the interaction is direct and leads to stabilize ELL2 and prevent ELL2 ubiquitination and degradation. Component of the super elongation complex (SEC), at least composed of EAF1, EAF2, CDK9, MLLT3/AF9, AFF (AFF1 or AFF4), the P-TEFb complex and ELL (ELL, ELL2 or ELL3). Interacts with ELL.

Family&Domains:

Belongs to the AF4 family.

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