ACOX3 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from MCF7 cells, using ACOX3 Antibody.

Product:	ACOX3 Antibody
Catalog:	DF9155
Description:	Rabbit polyclonal antibody to ACOX3
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Chicken, Xenopus
Mol.Wt.:	78kda,68kDa; 78kD(Calculated).
Uniprot:	O15254
RRID:	AB_2842351

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(83%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Chicken(92%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

ACOX3 Antibody detects endogenous levels of total ACOX3.

RRID:

AB_2842351
Cite Format: Affinity Biosciences Cat# DF9155, RRID:AB_2842351.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ACOX3; Acyl-CoA oxidase 3, pristanoyl; Acyl-CoA oxidase, pristanoyl, peroxisomal; Acyl-Coenzyme A oxidase 3, pristanoyl; Branched-chain acyl-CoA oxidase; BRCACox; BRCOX; EST-s59; PCOX; Peroxisomal acyl-coenzyme A oxidase 3; Pristanoyl-CoA oxidase;

Immunogens

Immunogen:

Uniprot:

O15254(ACOX3_HUMAN) >>Visit HPA database.

Gene(ID):

ACOX3(8310)

Sequence:

O15254 ACOX3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASTVEGGDTALLPEFPRGPLDAYRARASFSWKELALFTEGEGMLRFKKTIFSALENDPLFARSPGADLSLEKYRELNFLRCKRIFEYDFLSVEDMFKSPLKVPALIQCLGMYDSSLAAKYLLHSLVFGSAVYSSGSERHLTYIQKIFRMEIFGCFALTELSHGSNTKAIRTTAHYDPATEEFIIHSPDFEAAKFWVGNMGKTATHAVVFAKLCVPGDQCHGLHPFIVQIRDPKTLLPMPGVMVGDIGKKLGQNGLDNGFAMFHKVRVPRQSLLNRMGDVTPEGTYVSPFKDVRQRFGASLGSLSSGRVSIVSLAILNLKLAVAIALRFSATRRQFGPTEEEEIPVLEYPMQQWRLLPYLAAVYALDHFSKSLFLDLVELQRGLASGDRSARQAELGREIHALASASKPLASWTTQQGIQECREACGGHGYLAMNRLGVLRDDNDPNCTYEGDNNILLQQTSNYLLGLLAHQVHDGACFRSPLKSVDFLDAYPGILDQKFEVSSVADCLDSAVALAAYKWLVCYLLRETYQKLNQEKRSGSSDFEARNKCQVSHGRPLALAFVELTVVQRFHEHVHQPSVPPSLRAVLGRLSALYALWSLSRHAALLYRGGYFSGEQAGEVLESAVLALCSQLKDDAVALVDVIAPPDFVLDSPIGRADGELYKNLWGAVLQESKVLERASWWPEFSVNKPVIGSLKSKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Xenopus

100

Zebrafish

100

Chicken

Pig

Dog

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15254 As Substrate

O15254

Site	PTM Type	Source
A2	Acetylation	Uniprot
K49	Ubiquitination	Uniprot
S64	Phosphorylation	Uniprot
K73	Ubiquitination	Uniprot
Y74	Phosphorylation	Uniprot
S187	Phosphorylation	Uniprot
T235	Phosphorylation	Uniprot
K265	Ubiquitination	Uniprot
T281	Phosphorylation	Uniprot
Y286	Phosphorylation	Uniprot
S288	Phosphorylation	Uniprot
K291	Ubiquitination	Uniprot
K408	Ubiquitination	Uniprot
K484	Ubiquitination	Uniprot

Research Backgrounds

O15254_ACOX3_HUMAN

Function:

Oxidizes the CoA-esters of 2-methyl-branched fatty acids.

Subcellular Location:

Peroxisome.

Family&Domains:

Belongs to the acyl-CoA oxidase family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Metabolism > Lipid metabolism > Fatty acid degradation.

· Metabolism > Lipid metabolism > alpha-Linolenic acid metabolism.

· Metabolism > Lipid metabolism > Biosynthesis of unsaturated fatty acids.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Fatty acid metabolism.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

ACOX3 Antibody - #DF9155