ACOX2 Antibody | Affinity Biosciences

IHC
IF/ICC
Cites

1/3

DF9154 at 1/100 staining rat testis tissue by IHC-P.

Product:	ACOX2 Antibody
Catalog:	DF9154
Description:	Rabbit polyclonal antibody to ACOX2
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	77 kDa; 77kD(Calculated).
Uniprot:	Q99424
RRID:	AB_2842350

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(100%), Sheep(100%), Rabbit(90%), Dog(80%)

Clonality:

Polyclonal

Specificity:

ACOX2 Antibody detects endogenous levels of total ACOX2.

RRID:

AB_2842350
Cite Format: Affinity Biosciences Cat# DF9154, RRID:AB_2842350.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

12-alpha-trihydroxy-5-beta-cholestanoyl-CoA 24-hydroxylase; 12-alpha-trihydroxy-5-beta-cholestanoyl-CoA oxidase; 3 alpha 7 alpha 12 alpha trihydroxy 5 beta cholestanoyl CoA oxidase; 3 alpha 7 alpha12 alpha trihydroxy 5 beta cholestanoyl CoA 24 hydroxylase; 3-alpha; 7-alpha; ACOX 2; ACOX2; ACOX2_HUMAN; Acyl CoA oxidase 2; Acyl Coenzyme A oxidase 2; Acyl Coenzyme A oxidase 2 branched chain; Acyl coenzyme A oxidase 2 peroxisomal; BCOX; BRCACOX; BRCOX; Peroxisomal acyl-coenzyme A oxidase 2; Peroxisomal branched chain acyl CoA oxidase; THCA CoA oxidase; THCA-CoA oxidase; THCCox; Trihydroxycoprostanoyl CoA oxidase; Trihydroxycoprostanoyl-CoA oxidase;

Immunogens

Immunogen:

Uniprot:

Q99424(ACOX2_HUMAN) >>Visit HPA database.

Gene(ID):

ACOX2(8309)

Expression:

Q99424 ACOX2_HUMAN:

Present in all tissues tested: heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Most abundant in heart, liver and kidney.

Sequence:

Q99424 ACOX2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGSPVHRVSLGDTWSRQMHPDIESERYMQSFDVERLTNILDGGAQNTALRRKVESIIHSYPEFSCKDNYFMTQNERYKAAMRRAFHIRLIARRLGWLEDGRELGYAYRALSGDVALNIHRVFVRALRSLGSEEQIAKWDPLCKNIQIIATYAQTELGHGTYLQGLETEATYDAATQEFVIHSPTLTATKWWPGDLGRSATHALVQAQLICSGARRGMHAFIVPIRSLQDHTPLPGIIIGDIGPKMDFDQTDNGFLQLNHVRVPRENMLSRFAQVLPDGTYVKLGTAQSNYLPMVVVRVELLSGEILPILQKACVIAMRYSVIRRQSRLRPSDPEAKVLDYQTQQQKLFPQLAISYAFHFLAVSLLEFFQHSYTAILNQDFSFLPELHALSTGMKAMMSEFCTQGAEMCRRACGGHGYSKLSGLPSLVTKLSASCTYEGENTVLYLQVARFLVKSYLQTQMSPGSTPQRSLSPSVAYLTAPDLARCPAQRAADFLCPELYTTAWAHVAVRLIKDSVQHLQTLTQSGADQHEAWNQTTVIHLQAAKVHCYYVTVKGFTEALEKLENEPAIQQVLKRLCDLHAIHGILTNSGDFLHDAFLSGAQVDMARTAYLDLLRLIRKDAILLTDAFDFTDQCLNSALGCYDGNVYERLFQWAQKSPTNTQENPAYEEYIRPLLQSWRSKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

100

Rabbit

Dog

Pig

Horse

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99424 As Substrate

Q99424

Site	PTM Type	Source
S9	Phosphorylation	Uniprot
T13	Phosphorylation	Uniprot
S55	Phosphorylation	Uniprot
Y290	Phosphorylation	Uniprot
Y417	Phosphorylation	Uniprot

Research Backgrounds

Q99424_ACOX2_HUMAN

Function:

Oxidizes the CoA esters of the bile acid intermediates di- and tri-hydroxycholestanoic acids. Capable of oxidizing short as well as long chain 2-methyl branched fatty acids (By similarity).

Subcellular Location:

Peroxisome.

Tissue Specificity:

Present in all tissues tested: heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Most abundant in heart, liver and kidney.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the acyl-CoA oxidase family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)

· Metabolism > Lipid metabolism > Primary bile acid biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

References

1). Transcriptome analysis reveals the efficacy of ginsenoside-Rg1 in the treatment of nonalcoholic fatty liver disease. Life Sciences, 2021 (PubMed: 33385408) [IF=6.1]

Application: WB Species: rat Sample: liver tissue

Fig. 5. A) The protein expression levels of ATF3 and ACOX in rat liver of different groups. B) Atf3 relative protein expression level C) Acox2 relative protein expression level (*p < 0.05, **p < 0.01, ***p < 0.001, compare to NAFLD model group, SNK-Q test, n=10).

Restrictive clause

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For Research Use Only.
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ACOX2 Antibody - #DF9154