Product: ACOX2 Antibody
Catalog: DF9154
Description: Rabbit polyclonal antibody to ACOX2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 77 kDa; 77kD(Calculated).
Uniprot: Q99424
RRID: AB_2842350

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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Sheep(100%), Rabbit(90%), Dog(80%)
Clonality:
Polyclonal
Specificity:
ACOX2 Antibody detects endogenous levels of total ACOX2.
RRID:
AB_2842350
Cite Format: Affinity Biosciences Cat# DF9154, RRID:AB_2842350.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

12-alpha-trihydroxy-5-beta-cholestanoyl-CoA 24-hydroxylase; 12-alpha-trihydroxy-5-beta-cholestanoyl-CoA oxidase; 3 alpha 7 alpha 12 alpha trihydroxy 5 beta cholestanoyl CoA oxidase; 3 alpha 7 alpha12 alpha trihydroxy 5 beta cholestanoyl CoA 24 hydroxylase; 3-alpha; 7-alpha; ACOX 2; ACOX2; ACOX2_HUMAN; Acyl CoA oxidase 2; Acyl Coenzyme A oxidase 2; Acyl Coenzyme A oxidase 2 branched chain; Acyl coenzyme A oxidase 2 peroxisomal; BCOX; BRCACOX; BRCOX; Peroxisomal acyl-coenzyme A oxidase 2; Peroxisomal branched chain acyl CoA oxidase; THCA CoA oxidase; THCA-CoA oxidase; THCCox; Trihydroxycoprostanoyl CoA oxidase; Trihydroxycoprostanoyl-CoA oxidase;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q99424 ACOX2_HUMAN:

Present in all tissues tested: heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Most abundant in heart, liver and kidney.

Sequence:
MGSPVHRVSLGDTWSRQMHPDIESERYMQSFDVERLTNILDGGAQNTALRRKVESIIHSYPEFSCKDNYFMTQNERYKAAMRRAFHIRLIARRLGWLEDGRELGYAYRALSGDVALNIHRVFVRALRSLGSEEQIAKWDPLCKNIQIIATYAQTELGHGTYLQGLETEATYDAATQEFVIHSPTLTATKWWPGDLGRSATHALVQAQLICSGARRGMHAFIVPIRSLQDHTPLPGIIIGDIGPKMDFDQTDNGFLQLNHVRVPRENMLSRFAQVLPDGTYVKLGTAQSNYLPMVVVRVELLSGEILPILQKACVIAMRYSVIRRQSRLRPSDPEAKVLDYQTQQQKLFPQLAISYAFHFLAVSLLEFFQHSYTAILNQDFSFLPELHALSTGMKAMMSEFCTQGAEMCRRACGGHGYSKLSGLPSLVTKLSASCTYEGENTVLYLQVARFLVKSYLQTQMSPGSTPQRSLSPSVAYLTAPDLARCPAQRAADFLCPELYTTAWAHVAVRLIKDSVQHLQTLTQSGADQHEAWNQTTVIHLQAAKVHCYYVTVKGFTEALEKLENEPAIQQVLKRLCDLHAIHGILTNSGDFLHDAFLSGAQVDMARTAYLDLLRLIRKDAILLTDAFDFTDQCLNSALGCYDGNVYERLFQWAQKSPTNTQENPAYEEYIRPLLQSWRSKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Rabbit
90
Dog
80
Pig
70
Horse
60
Chicken
50
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99424 As Substrate

Site PTM Type Enzyme
S9 Phosphorylation
T13 Phosphorylation
S55 Phosphorylation
Y290 Phosphorylation
Y417 Phosphorylation

Research Backgrounds

Function:

Oxidizes the CoA esters of the bile acid intermediates di- and tri-hydroxycholestanoic acids. Capable of oxidizing short as well as long chain 2-methyl branched fatty acids (By similarity).

Subcellular Location:

Peroxisome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Present in all tissues tested: heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Most abundant in heart, liver and kidney.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the acyl-CoA oxidase family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

· Metabolism > Lipid metabolism > Primary bile acid biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

References

1). Transcriptome analysis reveals the efficacy of ginsenoside-Rg1 in the treatment of nonalcoholic fatty liver disease. Life Sciences, 2021 (PubMed: 33385408) [IF=6.1]

Application: WB    Species: rat    Sample: liver tissue

Fig. 5. A) The protein expression levels of ATF3 and ACOX in rat liver of different groups. B) Atf3 relative protein expression level C) Acox2 relative protein expression level (*p < 0.05, **p < 0.01, ***p < 0.001, compare to NAFLD model group, SNK-Q test, n=10).

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