Product: AWAT2 Antibody
Catalog: DF9149
Source: Rabbit
Application: WB, IHC, IF/ICC, ELISA(peptide)
Reactivity: Human, Mouse, Rat
Prediction: Rabbit, Dog
Mol.Wt.: 38 kD; 38kD(Calculated).
Uniprot: Q6E213
RRID: AB_2842345

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Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:1000-3000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(100%), Dog(88%)
Clonality:
Polyclonal
Specificity:
AWAT2 Antibody detects endogenous levels of total AWAT2.
RRID:
AB_2842345
Cite Format: Affinity Biosciences Cat# DF9149, RRID:AB_2842345.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Acyl CoA wax alcohol acyltransferase 2; Acyl-CoA retinol O-fatty-acyltransferase; Acyl-CoA wax alcohol acyltransferase 2; ARAT; Awat2; AWAT2_HUMAN; DC4; Diacylglycerol O acyltransferase 2 like 4; Diacylglycerol O acyltransferase candidate 4; Diacylglycerol O-acyltransferase 2-like protein 4; Diacylglycerol O-acyltransferase candidate 4; hDC4; hWS; Long chain alcohol O fatty acyltransferase 2; Long-chain-alcohol O-fatty-acyltransferase 2; MFAT; Multifunctional O acyltransferase; Multifunctional O-acyltransferase; Retinol O-fatty-acyltransferase; Wax synthase 2; Wax synthase; WS;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q6E213 AWAT2_HUMAN:

Highly expressed in skin, where it is primarily restricted to undifferentiated peripheral sebocytes. Also expressed at lower level in other tissues except pancreas.

Sequence:
MLLPSKKDLKTALDVFAVFQWSFSALLITTTVIAVNLYLVVFTPYWPVTVLILTWLAFDWKTPQRGGRRFTCVRHWRLWKHYSDYFPLKLLKTHDICPSRNYILVCHPHGLFAHGWFGHFATEASGFSKIFPGITPYILTLGAFFWMPFLREYVMSTGACSVSRSSIDFLLTHKGTGNMVIVVIGGLAECRYSLPGSSTLVLKNRSGFVRMALQHGVPLIPAYAFGETDLYDQHIFTPGGFVNRFQKWFQSMVHIYPCAFYGRGFTKNSWGLLPYSRPVTTIVGEPLPMPKIENPSQEIVAKYHTLYIDALRKLFDQHKTKFGISETQELEII

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Dog
88
Pig
75
Horse
75
Sheep
75
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6E213 As Substrate

Site PTM Type Enzyme
T176 Phosphorylation
S206 Phosphorylation

Research Backgrounds

Function:

Acyltransferase that catalyzes the formation of ester bonds between fatty alcohols and fatty acyl-CoAs to form wax monoesters. Shows a preference for medium chain acyl-CoAs from C12 to C16 in length and fatty alcohols shorter than C20, as the acyl donors and acceptors, respectively. Also possesses acyl-CoA retinol acyltransferase (ARAT) activity that catalyzes 11-cis-specific retinyl ester synthesis. Shows higher catalytic efficiency toward 11-cis-retinol versus 9-cis-retinol, 13-cis-retinol, and all-trans-retinol substrates.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in skin, where it is primarily restricted to undifferentiated peripheral sebocytes. Also expressed at lower level in other tissues except pancreas.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the diacylglycerol acyltransferase family.

Research Fields

· Metabolism > Metabolism of cofactors and vitamins > Retinol metabolism.

· Organismal Systems > Digestive system > Vitamin digestion and absorption.

Restrictive clause

 

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