ACAD9 Antibody - #DF9148
| Product: | ACAD9 Antibody |
| Catalog: | DF9148 |
| Description: | Rabbit polyclonal antibody to ACAD9 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 69kDa; 69kD(Calculated). |
| Uniprot: | Q9H845 |
| RRID: | AB_2842344 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9148, RRID:AB_2842344.
Fold/Unfold
Acyl CoA dehydrogenase 9; Acyl Coenzyme A dehydrogenase family, member 9; acyl-CoA dehydrogenase family member 9, mitochondrial; FLJ23533; MGC14452; NPD002; Very long chain acyl CoA dehydrogenase VLCAD;
Immunogens
Ubiquitously expressed in most normal human tissues and cancer cell lines with high level of expression in heart, skeletal muscles, brain, kidney and liver (PubMed:12359260). In the cerebellum uniquely expressed in the granular layer (at protein level) (PubMed:21237683).
- Q9H845 ACAD9_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGCGLFLRTTAAARACRGLVVSTANRRLLRTSPPVRAFAKELFLGKIKKKEVFPFPEVSQDELNEINQFLGPVEKFFTEEVDSRKIDQEGKIPDETLEKLKSLGLFGLQVPEEYGGLGFSNTMYSRLGEIISMDGSITVTLAAHQAIGLKGIILAGTEEQKAKYLPKLASGEHIAAFCLTEPASGSDAASIRSRATLSEDKKHYILNGSKVWITNGGLANIFTVFAKTEVVDSDGSVKDKITAFIVERDFGGVTNGKPEDKLGIRGSNTCEVHFENTKIPVENILGEVGDGFKVAMNILNSGRFSMGSVVAGLLKRLIEMTAEYACTRKQFNKRLSEFGLIQEKFALMAQKAYVMESMTYLTAGMLDQPGFPDCSIEAAMVKVFSSEAAWQCVSEALQILGGLGYTRDYPYERILRDTRILLIFEGTNEILRMYIALTGLQHAGRILTTRIHELKQAKVSTVMDTVGRRLRDSLGRTVDLGLTGNHGVVHPSLADSANKFEENTYCFGRTVETLLLRFGKTIMEEQLVLKRVANILINLYGMTAVLSRASRSIRIGLRNHDHEVLLANTFCVEAYLQNLFSLSQLDKYAPENLDEQIKKVSQQILEKRAYICAHPLDRTC
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H845 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T11 | Phosphorylation | Uniprot | |
| K41 | Acetylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| T158 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| T229 | Phosphorylation | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| R266 | Methylation | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| S309 | Phosphorylation | Uniprot | |
| Y325 | Phosphorylation | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| S474 | Phosphorylation | Uniprot | |
| K599 | Ubiquitination | Uniprot | |
| K608 | Acetylation | Uniprot | |
| K608 | Ubiquitination | Uniprot | |
| Y611 | Phosphorylation | Uniprot | |
| R619 | Methylation | Uniprot |
Research Backgrounds
As part of the MCIA complex, primarily participates to the assembly of the mitochondrial complex I and therefore plays a role in oxidative phosphorylation. This moonlighting protein has also a dehydrogenase activity toward a broad range of substrates with greater specificity for long-chain unsaturated acyl-CoAs. However, in vivo, it does not seem to play a primary role in fatty acid oxidation. In addition, the function in complex I assembly is independent of the dehydrogenase activity of the protein.
Mitochondrion inner membrane>Peripheral membrane protein>Matrix side.
Note: Essentially associated with membranes.
Ubiquitously expressed in most normal human tissues and cancer cell lines with high level of expression in heart, skeletal muscles, brain, kidney and liver. In the cerebellum uniquely expressed in the granular layer (at protein level).
Homodimer. Part of the mitochondrial complex I assembly/MCIA complex that comprises at least TMEM126B, NDUFAF1, ECSIT, and ACAD9 (By similarity). Interacts with NDUFAF1 and ECSIT.
Belongs to the acyl-CoA dehydrogenase family.
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