Product: RPL7A Antibody
Catalog: DF9137
Description: Rabbit polyclonal antibody to RPL7A
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 30kDa; 30kD(Calculated).
Uniprot: P62424
RRID: AB_2842333

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(100%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
RPL7A Antibody detects endogenous levels of total RPL7A.
RRID:
AB_2842333
Cite Format: Affinity Biosciences Cat# DF9137, RRID:AB_2842333.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

60S ribosomal protein L7a; L7A; MGC103146; PLA X polypeptide; PLA-X polypeptide; ribosomal protein L7a; RL7A_HUMAN; RP23-414L19.4; RPL7A; SURF 3; SURF-3; SURF3; surfeit 3; Surfeit locus protein 3; Thyroid hormone receptor uncoupling protein; TRUP;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MPKGKKAKGKKVAPAPAVVKKQEAKKVVNPLFEKRPKNFGIGQDIQPKRDLTRFVKWPRYIRLQRQRAILYKRLKVPPAINQFTQALDRQTATQLLKLAHKYRPETKQEKKQRLLARAEKKAAGKGDVPTKRPPVLRAGVNTVTTLVENKKAQLVVIAHDVDPIELVVFLPALCRKMGVPYCIIKGKARLGRLVHRKTCTTVAFTQVNSEDKGALAKLVEAIRTNYNDRYDEIRRHWGGNVLGPKSVARIAKLEKAKAKELATKLG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Xenopus
92
Pig
0
Horse
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P62424 As Substrate

Site PTM Type Enzyme
K11 Ubiquitination
K20 Sumoylation
K20 Ubiquitination
K26 Ubiquitination
K34 Acetylation
K34 Ubiquitination
K37 Ubiquitination
K48 Acetylation
K48 Sumoylation
K48 Ubiquitination
K56 Ubiquitination
Y71 Phosphorylation
K72 Ubiquitination
K75 Ubiquitination
R89 Methylation
K97 Acetylation
K97 Ubiquitination
K101 Acetylation
K101 Ubiquitination
T106 Phosphorylation
K107 Ubiquitination
K121 Ubiquitination
K125 Ubiquitination
K131 Ubiquitination
K150 Acetylation
K150 Ubiquitination
K176 Sumoylation
K176 Ubiquitination
Y181 Phosphorylation
C182 S-Nitrosylation
K185 Ubiquitination
K197 Ubiquitination
T198 Phosphorylation
S209 Phosphorylation
K212 Acetylation
K212 Ubiquitination
K217 Acetylation
K217 Ubiquitination
R223 Methylation
R229 Methylation
Y230 Phosphorylation
R234 Methylation
R235 Methylation
K245 Ubiquitination
K252 Sumoylation
K255 Ubiquitination
K259 Ubiquitination
K264 Sumoylation
K264 Ubiquitination

Research Backgrounds

Subunit Structure:

Interacts with CRY1 (By similarity). Interacts with DICER1, AGO2, TARBP2, MOV10 and EIF6; they form a large RNA-induced silencing complex (RISC).

Family&Domains:

Belongs to the eukaryotic ribosomal protein eL8 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

References

1). SOX1 acts as a tumor hypnotist rendering nasopharyngeal carcinoma cells refractory to chemotherapy. Cell Death Discovery, 2023 (PubMed: 37369660) [IF=7.0]

Application: WB    Species: Human    Sample: NPC cells

Fig. 1 SOX1 decreases ribosome pathway and DNA replication in NPC cells. A An experimental timeline for doxycycline (Blue block: 0 µg/ml, red block: 1 µg/ml) schedules used to control the expression of SOX1 in cells (HONE1 TRE-SOX1 or CNE2 TRE-SOX1). On day 2 and day 4, the cells were counted and proteins were extracted for protein concentration and western blot analysis, respectively. B Western blot analysis of SOX1, RPS3, RPL7A, and β-actin expression in cells. β-actin was used as a control. C Gene set enrichment analysis (GSEA) of ranked genes from cells. Gene set “hsa03010: Ribosome” of KEGG pathways was presented. NES normalized enrichment score. D Scatter dot plots showing the average protein content per cell. E An experimental timeline for doxycycline (Blue block: 0 µg/ml, red block: 1 µg/ml) schedules used to control the expression of SOX1 in cells (HONE1 TRE-SOX1 or CNE2 TRE-SOX1). Both groups of cells were treated with 20 µM BrdU for varying durations, and on day 4, BrdU-positive cells were determined by flow cytometry analysis. F Histograms showing the percentage of BrdU-negative or -positive cells in “SOX1-High” and “SOX1-Low” groups of cells. G Time courses of the percentage of BrdU-positive cells in BrdU incorporation assays, which were fitted by an exponential plateau equation. H An illustration of model hypothesis that overexpression of SOX1 induces quiescent NPC cells. Dox Doxycycline.

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