RPL4 Antibody - #DF9135

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Product: RPL4 Antibody
Catalog: DF9135
Description: Rabbit polyclonal antibody to RPL4
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 48 kDa; 48kD(Calculated).
Uniprot: P36578
RRID: AB_2842331

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RPL4 Antibody detects endogenous levels of total RPL4.
RRID:
AB_2842331
Cite Format: Affinity Biosciences Cat# DF9135, RRID:AB_2842331.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

60S ribosomal protein L1; 60S ribosomal protein L4; L4; Ribosomal protein L4; RL4_HUMAN; rpl4;

Immunogens

Immunogen:
Uniprot:

P36578(RL4_HUMAN) >>Visit HPA database.

Gene(ID):
RPL4(6124)
Sequence:
MACARPLISVYSEKGESSGKNVTLPAVFKAPIRPDIVNFVHTNLRKNNRQPYAVSELAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKTWRRWHRRVNTTQKRYAICSALAASALPALVMSKGHRIEEVPELPLVVEDKVEGYKKTKEAVLLLKKLKAWNDIKKVYASQRMRAGKGKMRNRRRIQRRGPCIIYNEDNGIIKAFRNIPGITLLNVSKLNILKLAPGGHVGRFCIWTESAFRKLDELYGTWRKAASLKSNYNLPMHKMINTDLSRILKSPEIQRALRAPRKKIHRRVLKKNPLKNLRIMLKLNPYAKTMRRNTILRQARNHKLRVDKAAAAAAALQAKSDEKAAVAGKKPVVGKKGKKAAVGVKKQKKPLVGKKAAATKKPAPEKKPAEKKPTTEEKKPAA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P36578 As Substrate

Site PTM Type Enzyme
A2 Acetylation
Y11 Phosphorylation
S12 Phosphorylation
K14 Acetylation
K14 Ubiquitination
K20 Ubiquitination
T23 Phosphorylation
K29 Acetylation
K29 Methylation
K29 Ubiquitination
K46 Ubiquitination
Y52 Phosphorylation
S55 Phosphorylation
T62 Phosphorylation
T69 Phosphorylation
R71 Methylation
R80 Methylation
R86 Methylation
S87 Phosphorylation
C96 S-Nitrosylation
R97 Methylation
R100 Methylation
K106 Acetylation
K106 Ubiquitination
Y122 Phosphorylation
S126 Phosphorylation
S131 Phosphorylation
S139 Phosphorylation
K140 Ubiquitination
K157 Acetylation
K157 Ubiquitination
K162 Acetylation
K162 Ubiquitination
K163 Acetylation
K163 Ubiquitination
K165 Ubiquitination
K172 Ubiquitination
K173 Ubiquitination
K175 Ubiquitination
K181 Ubiquitination
K182 Ubiquitination
Y184 Phosphorylation
S186 Phosphorylation
R188 Methylation
R205 Methylation
C208 S-Nitrosylation
K219 Ubiquitination
T228 Phosphorylation
S233 Phosphorylation
K234 Ubiquitination
K239 Ubiquitination
C250 S-Nitrosylation
S255 Phosphorylation
K259 Ubiquitination
Y264 Phosphorylation
T266 Phosphorylation
K269 Ubiquitination
S272 Phosphorylation
K274 Acetylation
K274 Methylation
K274 Ubiquitination
Y277 Phosphorylation
K283 Methylation
K283 Ubiquitination
S290 Phosphorylation
K294 Ubiquitination
S295 Phosphorylation
K320 Ubiquitination
K327 Ubiquitination
Y331 Phosphorylation
K333 Acetylation
K333 Ubiquitination
T334 Phosphorylation
T339 Phosphorylation
K353 Ubiquitination
K364 Sumoylation
K364 Ubiquitination
S365 Phosphorylation
K368 Acetylation
K368 Ubiquitination
K374 Ubiquitination
K380 Ubiquitination
K405 Ubiquitination
K412 Acetylation
T419 Phosphorylation
T420 Phosphorylation

Research Backgrounds

PTMs:

Citrullinated by PADI4.

Subunit Structure:

May bind IPO9 with low affinity. Interacts with RBM3 (By similarity).

Family&Domains:

Belongs to the universal ribosomal protein uL4 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

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