Product: RPL35A Antibody
Catalog: DF9131
Description: Rabbit polyclonal antibody to RPL35A
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 13 kDa; 13kD(Calculated).
Uniprot: P18077
RRID: AB_2842327

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RPL35A Antibody detects endogenous levels of total RPL35A.
RRID:
AB_2842327
Cite Format: Affinity Biosciences Cat# DF9131, RRID:AB_2842327.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

60S ribosomal protein L35a; Cell growth-inhibiting gene 33 protein; DBA5; L35A; Ribosomal Protein L35a; RL35A_HUMAN; RPL35A;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MSGRLWSKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPGGKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRIRVMLYPSRI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P18077 As Substrate

Site PTM Type Enzyme
R4 Methylation
K8 Acetylation
K8 Ubiquitination
Y14 Phosphorylation
K15 Acetylation
K15 Ubiquitination
T25 Phosphorylation
K29 Acetylation
K29 Ubiquitination
Y34 Phosphorylation
Y42 Phosphorylation
K45 Acetylation
K45 Ubiquitination
K52 Methylation
K52 Ubiquitination
K54 Ubiquitination
T59 Phosphorylation
K63 Ubiquitination
K66 Ubiquitination
K73 Ubiquitination
K95 Ubiquitination
Y106 Phosphorylation
R109 Methylation

Research Backgrounds

Function:

Required for the proliferation and viability of hematopoietic cells. Plays a role in 60S ribosomal subunit formation. The protein was found to bind to both initiator and elongator tRNAs and consequently was assigned to the P site or P and A site.

Family&Domains:

Belongs to the eukaryotic ribosomal protein eL33 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

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For Research Use Only.
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