Product: RPL27 Antibody
Catalog: DF9129
Description: Rabbit polyclonal antibody to RPL27
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 16 kDa; 16kD(Calculated).
Uniprot: P61353
RRID: AB_2842325

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
RPL27 Antibody detects endogenous levels of total RPL27.
RRID:
AB_2842325
Cite Format: Affinity Biosciences Cat# DF9129, RRID:AB_2842325.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

60S ribosomal protein L27; Ribosomal protein L27; RPL 27;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MGKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Xenopus
92
Zebrafish
92
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P61353 As Substrate

Site PTM Type Enzyme
K6 Ubiquitination
K9 Ubiquitination
K27 Acetylation
K27 Ubiquitination
R36 Methylation
Y49 Phosphorylation
K52 Ubiquitination
K59 Ubiquitination
K73 Ubiquitination
Y75 Phosphorylation
Y77 Phosphorylation
T83 Phosphorylation
R84 Methylation
S86 Phosphorylation
K93 Acetylation
K93 Ubiquitination
K98 Acetylation
K98 Sumoylation
K98 Ubiquitination
K107 Ubiquitination
K115 Ubiquitination
K117 Acetylation
K117 Sumoylation
K117 Ubiquitination
K128 Acetylation
K128 Ubiquitination
K133 Acetylation
K133 Ubiquitination

Research Backgrounds

Function:

Component of the large ribosomal subunit. Required for proper rRNA processing and maturation of 28S and 5.8S rRNAs.

Subcellular Location:

Cytoplasm>Cytosol. Cytoplasm. Rough endoplasmic reticulum.
Note: Detected on cytosolic polysomes (PubMed:25957688). Detected in ribosomes that are associated with the rough endoplasmic reticulum (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the large ribosomal subunit. Interacts with RRP1B. Interacts with DHX33.

Family&Domains:

Belongs to the eukaryotic ribosomal protein eL27 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.