RPL13A Antibody - #DF9126

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Product: RPL13A Antibody
Catalog: DF9126
Description: Rabbit polyclonal antibody to RPL13A
Application: WB
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Zebrafish, Bovine, Horse, Dog, Xenopus
Mol.Wt.: 24 kDa; 24kD(Calculated).
Uniprot: P40429
RRID: AB_2842322

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RPL13A Antibody detects endogenous levels of total RPL13A.
RRID:
AB_2842322
Cite Format: Affinity Biosciences Cat# DF9126, RRID:AB_2842322.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

23 kD highly basic protein; 23 kDa highly basic protein; 60S ribosomal protein L13a; L13A; P198; Ribosomal protein L13a; RPL 13A; Tissue specific transplantation antigen 1; Transplantation antigen P198; TSTA1; Tstap198 7; Tum antigen; Tum P198 antigen;

Immunogens

Immunogen:
Uniprot:

P40429(RL13A_HUMAN) >>Visit HPA database.

Gene(ID):
RPL13A(23521)
Sequence:
MAEVQVLVLDGRGHLLGRLAAIVAKQVLLGRKVVVVRCEGINISGNFYRNKLKYLAFLRKRMNTNPSRGPYHFRAPSRIFWRTVRGMLPHKTKRGQAALDRLKVFDGIPPPYDKKKRMVVPAALKVVRLKPTRKFAYLGRLAHEVGWKYQAVTATLEEKRKEKAKIHYRKKKQLMRLRKQAEKNVEKKIDKYTEVLKTHGLLV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Sheep
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P40429 As Substrate

Site PTM Type Enzyme
Phosphorylation
A2 Acetylation
R12 Methylation
K25 Acetylation
K25 Ubiquitination
C38 S-Nitrosylation
K51 Ubiquitination
K53 Ubiquitination
R68 Methylation
R74 Methylation
S77 Phosphorylation P53355 (DAPK1) , O43293 (DAPK3) , PR:000037784 (hDAPK3/Phos:1)
K91 Ubiquitination
R101 Methylation
K103 Ubiquitination
K114 Ubiquitination
K115 Ubiquitination
K125 Acetylation
K125 Ubiquitination
K134 Ubiquitination
Y137 Phosphorylation
K148 Acetylation
K148 Methylation
K148 Ubiquitination
Y149 Phosphorylation
T153 Phosphorylation
T155 Phosphorylation
K159 Ubiquitination
K163 Acetylation
K165 Ubiquitination
K188 Ubiquitination
K191 Acetylation
K191 Ubiquitination
T193 Phosphorylation
K197 Acetylation
K197 Ubiquitination

Research Backgrounds

Function:

Associated with ribosomes but is not required for canonical ribosome function and has extra-ribosomal functions. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the ribosome and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. In the GAIT complex interacts with m7G cap-bound eIF4G at or near the eIF3-binding site and blocks the recruitment of the 43S ribosomal complex. Involved in methylation of rRNA.

PTMs:

Phosphorylation at Ser-77 upon interferon-gamma treatment in monocytes involves a DAPK1-DAPK3 kinase cascade and is causing release from the ribosome, association with the GAIT complex and subsequent involvement in transcript-selective translation inhibition.

Citrullinated by PADI4.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the 60S ribosome. Component of the GAIT complex. Interacts with EIF4G1.

Family&Domains:

Belongs to the universal ribosomal protein uL13 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

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