RPS3A Antibody - #DF9123

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Product: RPS3A Antibody
Catalog: DF9123
Description: Rabbit polyclonal antibody to RPS3A
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 30kDa; 30kD(Calculated).
Uniprot: P61247
RRID: AB_2842319

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(92%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RPS3A Antibody detects endogenous levels of total RPS3A.
RRID:
AB_2842319
Cite Format: Affinity Biosciences Cat# DF9123, RRID:AB_2842319.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

40S ribosomal protein S3a; Fte 1; Fte-1; FTE1; MFTL; ribosomal protein S3A; RPS3A; RS3A_HUMAN; S3A; v fos transformation effector protein 1; v fos transformation effector protein; V-fos transformation effector protein;

Immunogens

Immunogen:
Uniprot:

P61247(RS3A_HUMAN) >>Visit HPA database.

Gene(ID):
RPS3A(6189)
Sequence:
MAVGKNKRLTKGGKKGAKKKVVDPFSKKDWYDVKAPAMFNIRNIGKTLVTRTQGTKIASDGLKGRVFEVSLADLQNDEVAFRKFKLITEDVQGKNCLTNFHGMDLTRDKMCSMVKKWQTMIEAHVDVKTTDGYLLRLFCVGFTKKRNNQIRKTSYAQHQQVRQIRKKMMEIMTREVQTNDLKEVVNKLIPDSIGKDIEKACQSIYPLHDVFVRKVKMLKKPKFELGKLMELHGEGSSSGKATGDETGAKVERADGYEPPVQESV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
92
Dog
92
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P61247 As Substrate

Site PTM Type Enzyme
T10 Phosphorylation
S26 Phosphorylation
K27 Acetylation
K27 Methylation
K27 Ubiquitination
K28 Ubiquitination
Y31 Phosphorylation
K34 Acetylation
K34 Sumoylation
K34 Ubiquitination
K46 Acetylation
K46 Ubiquitination
K56 Ubiquitination
K63 Ubiquitination
K83 Ubiquitination
K85 Acetylation
K85 Ubiquitination
T88 Phosphorylation
K94 Acetylation
K94 Ubiquitination
K109 Acetylation
K109 Ubiquitination
S112 Phosphorylation
Y133 Phosphorylation
C139 S-Nitrosylation
K144 Acetylation
K144 Methylation
K152 Ubiquitination
K167 Ubiquitination
T173 Phosphorylation
K182 Acetylation
K182 Ubiquitination
K187 Ubiquitination
K195 Acetylation
K195 Ubiquitination
K199 Ubiquitination
C201 S-Nitrosylation
Y205 Phosphorylation
K220 Ubiquitination
K227 Ubiquitination
S236 Phosphorylation
S237 Phosphorylation
S238 Phosphorylation
K240 Ubiquitination
T242 Phosphorylation
T246 Phosphorylation
K249 Acetylation
K249 Sumoylation
K249 Ubiquitination
Y256 Phosphorylation
S263 Phosphorylation

Research Backgrounds

Function:

May play a role during erythropoiesis through regulation of transcription factor DDIT3.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (28S, 5.8S and 5S). Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Binds with high affinity to IPO4. Interacts with DDIT3.

Family&Domains:

Belongs to the eukaryotic ribosomal protein eS1 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

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