Product: RPS27 Antibody
Catalog: DF9122
Description: Rabbit polyclonal antibody to RPS27
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Dog, Chicken, Xenopus
Mol.Wt.: 9 kDa; 9kD(Calculated).
Uniprot: P42677
RRID: AB_2842318

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RPS27 Antibody detects endogenous levels of total RPS27.
RRID:
AB_2842318
Cite Format: Affinity Biosciences Cat# DF9122, RRID:AB_2842318.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2610206D02Rik; 3200001M24Rik; 40S ribosomal protein S27; C530005D02Rik; Metallopan stimulin 1; Metallopan-stimulin 1; Metallopanstimulin 1; Metallopanstimulin1; MPS 1; MPS-1; MPS1; pmn; Ribosomal protein S27; Ribosomal protein S27 metallopanstimulin 1; rps27; RS27_HUMAN; S27; snoRNA MBI 112;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P42677 RS27_HUMAN:

Expressed in a wide variety of actively proliferating cells and tumor tissues.

Sequence:
MPLAKDLLHPSPEEEKRKHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQTVVLCVGCSTVLCQPTGGKARLTEGCSFRRKQH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Chicken
100
Sheep
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P42677 As Substrate

Site PTM Type Enzyme
K5 Acetylation
K5 Methylation
K5 Sumoylation
K5 Ubiquitination
S11 Phosphorylation
K16 Acetylation
K16 Ubiquitination
K18 Ubiquitination
K22 Sumoylation
S27 Phosphorylation P24941 (CDK2) , Q16539 (MAPK14)
S30 Phosphorylation
Y31 Phosphorylation
K70 Ubiquitination
R72 Methylation
C77 S-Nitrosylation
S78 Phosphorylation

Research Backgrounds

Function:

Component of the small ribosomal subunit. Required for proper rRNA processing and maturation of 18S rRNAs.

Tissue Specificity:

Expressed in a wide variety of actively proliferating cells and tumor tissues.

Subunit Structure:

Component of the small ribosomal subunit.

Family&Domains:

Belongs to the eukaryotic ribosomal protein eS27 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.