Product: RPS21 Antibody
Catalog: DF9119
Description: Rabbit polyclonal antibody to RPS21
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 9 kDa; 9kD(Calculated).
Uniprot: P63220
RRID: AB_2842315

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(83%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
RPS21 Antibody detects endogenous levels of total RPS21.
RRID:
AB_2842315
Cite Format: Affinity Biosciences Cat# DF9119, RRID:AB_2842315.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1810049N11Rik; 2410030A14Rik; 40S ribosomal protein S21; 8.2 kDa differentiation factor; MGC107620; OTTHUMP00000031485; Ribosomal protein S21; RP23-16P3.4; RP5-908M14.8;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MQNDAGEFVDLYVPRKCSASNRIIGAKDHASIQMNVAEVDKVTGRFNGQFKTYAICGAIRRMGESDDSILRLAKADGIVSKNF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
92
Zebrafish
83
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P63220 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K27 Acetylation
K27 Ubiquitination
K41 Acetylation
K41 Sumoylation
K41 Ubiquitination
K51 Acetylation
K51 Methylation
K51 Ubiquitination
T52 Phosphorylation
Y53 Phosphorylation
C56 S-Nitrosylation
S65 Phosphorylation
S68 Phosphorylation
R71 Methylation
K74 Ubiquitination
S80 Phosphorylation
K81 Acetylation
K81 Ubiquitination

Research Backgrounds

Subcellular Location:

Cytoplasm>Cytosol. Cytoplasm. Rough endoplasmic reticulum.
Note: Detected on cytosolic polysomes (PubMed:25957688). Detected in ribosomes that are associated with the rough endoplasmic reticulum (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the 40S small ribosomal subunit.

Family&Domains:

Belongs to the eukaryotic ribosomal protein eS21 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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