RPS15A Antibody - #DF9117
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| Product: | RPS15A Antibody |
| Catalog: | DF9117 |
| Description: | Rabbit polyclonal antibody to RPS15A |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 15 kDa; 15kD(Calculated). |
| Uniprot: | P62244 |
| RRID: | AB_2842313 |
Product Info
Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RPS15A Antibody detects endogenous levels of total RPS15A.
RRID:
AB_2842313
Cite Format: Affinity Biosciences Cat# DF9117, RRID:AB_2842313.
Cite Format: Affinity Biosciences Cat# DF9117, RRID:AB_2842313.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
40S ribosomal protein S15a; FLJ27457; MGC111208; OK/SW-cl.82; Ribosomal protein S15a; S15a; Upregulated by HBV X protein;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- P62244 RS15A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P62244 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K12 | Ubiquitination | Uniprot | |
| K19 | Acetylation | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| T66 | Phosphorylation | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| K88 | Acetylation | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| T121 | Phosphorylation | Uniprot | |
| K124 | Acetylation | Uniprot | |
| K124 | Ubiquitination | Uniprot |
Research Backgrounds
Function:
Structural component of the ribosome. Required for proper erythropoiesis.
Subunit Structure:
Component of the 40S ribosomal subunit.
Family&Domains:
Belongs to the universal ribosomal protein uS8 family.
Research Fields
· Genetic Information Processing > Translation > Ribosome.
References
1). m6A‑modified HOXC10 promotes HNSCC progression via co‑activation of ADAM17/EGFR and Wnt/β‑catenin signaling. International journal of oncology, 2024
(PubMed: 38063205)
[IF=5.2]
Application: WB Species: Human Sample: HNSCC cells
Figure 5 HOXC10 facilitates Wnt/β-catenin signaling in HNSCC by interacting with RPS15A. (A-C) The binding partners of HOXC10 were analyzed by the combination of Co-IP and mass spectrometric analyses, and 11 proteins are presented. (D) Co-IP and western blot analysis were used to verify the interaction between HOXC10 and RPS15A. (E) Immunofluorescence was used to identify the colocalization of HOXC10 and RPS15A. Scale bar, 25 µm. (F and G) RT-qPCR and western blot analysis of RPS15A expression in HNSCC cells transfected with shRNA2 or HOXC10. (H) Ubiquitination assay for the effects of HOXC10 on RPS15A ubiquitination. (I) GSEA based on the TCGA dataset suggested that HOXC10 expression was positively associated with the activation of the Wnt/β-catenin pathway. (J) The efficiency of transfection in the indicated cells was confirmed by RT-qPCR and western blotting. (K) Immunofluorescence detection of β-catenin in the nucleus after transfection with shRNA2, HOXC10, RPS15A, or siRPS15A in indicated cells. Scale bar, 25 µm. (L) Western blot analysis was performed to detect β-catenin, Myc, MMP7, Axin2, and RPS15A protein in HNSCC cells transfected with shRNA2, HOXC10, RPS15A, or siRPS15A. (M) Myc, MMP7, and Axin2 mRNA levels were analyzed by RT-qPCR in transfected HNSCC cells. (N) The activity of the Wnt/β-catenin pathway was analyzed by TOP/FOP-Flash luciferase reporter assay. (O and P) Migration and invasion ability in transfected HNSCC cells were assessed by Transwell assay. Scale bar, 100 µm **P
Application: IF/ICC Species: Human Sample: HNSCC cells
Figure 5 HOXC10 facilitates Wnt/β-catenin signaling in HNSCC by interacting with RPS15A. (A-C) The binding partners of HOXC10 were analyzed by the combination of Co-IP and mass spectrometric analyses, and 11 proteins are presented. (D) Co-IP and western blot analysis were used to verify the interaction between HOXC10 and RPS15A. (E) Immunofluorescence was used to identify the colocalization of HOXC10 and RPS15A. Scale bar, 25 µm. (F and G) RT-qPCR and western blot analysis of RPS15A expression in HNSCC cells transfected with shRNA2 or HOXC10. (H) Ubiquitination assay for the effects of HOXC10 on RPS15A ubiquitination. (I) GSEA based on the TCGA dataset suggested that HOXC10 expression was positively associated with the activation of the Wnt/β-catenin pathway. (J) The efficiency of transfection in the indicated cells was confirmed by RT-qPCR and western blotting. (K) Immunofluorescence detection of β-catenin in the nucleus after transfection with shRNA2, HOXC10, RPS15A, or siRPS15A in indicated cells. Scale bar, 25 µm. (L) Western blot analysis was performed to detect β-catenin, Myc, MMP7, Axin2, and RPS15A protein in HNSCC cells transfected with shRNA2, HOXC10, RPS15A, or siRPS15A. (M) Myc, MMP7, and Axin2 mRNA levels were analyzed by RT-qPCR in transfected HNSCC cells. (N) The activity of the Wnt/β-catenin pathway was analyzed by TOP/FOP-Flash luciferase reporter assay. (O and P) Migration and invasion ability in transfected HNSCC cells were assessed by Transwell assay. Scale bar, 100 µm **P
Application: IHC Species: Human Sample: HNSCC cells
Figure 5 HOXC10 facilitates Wnt/β-catenin signaling in HNSCC by interacting with RPS15A. (A-C) The binding partners of HOXC10 were analyzed by the combination of Co-IP and mass spectrometric analyses, and 11 proteins are presented. (D) Co-IP and western blot analysis were used to verify the interaction between HOXC10 and RPS15A. (E) Immunofluorescence was used to identify the colocalization of HOXC10 and RPS15A. Scale bar, 25 µm. (F and G) RT-qPCR and western blot analysis of RPS15A expression in HNSCC cells transfected with shRNA2 or HOXC10. (H) Ubiquitination assay for the effects of HOXC10 on RPS15A ubiquitination. (I) GSEA based on the TCGA dataset suggested that HOXC10 expression was positively associated with the activation of the Wnt/β-catenin pathway. (J) The efficiency of transfection in the indicated cells was confirmed by RT-qPCR and western blotting. (K) Immunofluorescence detection of β-catenin in the nucleus after transfection with shRNA2, HOXC10, RPS15A, or siRPS15A in indicated cells. Scale bar, 25 µm. (L) Western blot analysis was performed to detect β-catenin, Myc, MMP7, Axin2, and RPS15A protein in HNSCC cells transfected with shRNA2, HOXC10, RPS15A, or siRPS15A. (M) Myc, MMP7, and Axin2 mRNA levels were analyzed by RT-qPCR in transfected HNSCC cells. (N) The activity of the Wnt/β-catenin pathway was analyzed by TOP/FOP-Flash luciferase reporter assay. (O and P) Migration and invasion ability in transfected HNSCC cells were assessed by Transwell assay. Scale bar, 100 µm **P
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