RPS10 Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using RPS10 Antibody.

Product:	RPS10 Antibody
Catalog:	DF9116
Description:	Rabbit polyclonal antibody to RPS10
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	19 kDa; 19kD(Calculated).
Uniprot:	P46783
RRID:	AB_2842312

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Zebrafish(92%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(90%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

RPS10 Antibody detects endogenous levels of total RPS10.

RRID:

AB_2842312
Cite Format: Affinity Biosciences Cat# DF9116, RRID:AB_2842312.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

40S ribosomal protein S10; DBA9; MGC88819; OTTHUMP00000016229; OTTHUMP00000016230; RPS10; RS10_HUMAN; S10;

Immunogens

Immunogen:

Uniprot:

P46783(RS10_HUMAN) >>Visit HPA database.

Gene(ID):

RPS10(6204)

Sequence:

P46783 RS10_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIQYLRDYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARLTRGEADRDTYRRSAVPPGADKKAEAGAGSATEFQFRGGFGRGRGQPPQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Dog

100

Xenopus

100

Rabbit

100

Zebrafish

Chicken

Pig

Sheep

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P46783 As Substrate

P46783

Site	PTM Type	Enzyme	Source
Y12	Phosphorylation		Uniprot
K17	Acetylation		Uniprot
K17	Ubiquitination		Uniprot
K24	Acetylation		Uniprot
K24	Ubiquitination		Uniprot
K31	Ubiquitination		Uniprot
K38	Acetylation		Uniprot
K38	Ubiquitination		Uniprot
K47	Ubiquitination		Uniprot
K53	Ubiquitination		Uniprot
K59	Acetylation		Uniprot
K59	Ubiquitination		Uniprot
Y78	Phosphorylation		Uniprot
Y82	Phosphorylation		Uniprot
T93	Phosphorylation		Uniprot
T101	Phosphorylation		Uniprot
K107	Sumoylation		Uniprot
K107	Ubiquitination		Uniprot
T118	Phosphorylation	Q96GD4 (AURKB)	Uniprot
R119	Methylation		Uniprot
T126	Phosphorylation		Uniprot
Y127	Phosphorylation		Uniprot
S130	Phosphorylation		Uniprot
K138	Sumoylation		Uniprot
K138	Ubiquitination		Uniprot
K139	Methylation		Uniprot
K139	Sumoylation		Uniprot
K139	Ubiquitination		Uniprot
S146	Phosphorylation		Uniprot
T148	Phosphorylation		Uniprot
R153	Methylation		Uniprot
R158	Methylation		Uniprot
R160	Methylation		Uniprot

Research Backgrounds

P46783_RS10_HUMAN

Function:

Component of the 40S ribosomal subunit.

PTMs:

Methylated by PRMT5. Methylation is necessary for its interaction with NPS1, its localization in the granular component (GC) region of the nucleolus, for the proper assembly of ribosomes, protein synthesis and optimal cell proliferation.

Monoubiquitinated by ZNF598 when a ribosome has stalled during translation of poly(A) sequences, leading to preclude synthesis of a long poly-lysine tail and initiate the ribosome quality control (RQC) pathway to degrade the potentially detrimental aberrant nascent polypeptide.

Subcellular Location:

Cytoplasm. Nucleus>Nucleolus.
Note: Localized in the granular component (GC) region of the nucleolus. Methylation is required for its localization in the GC region. Colocalizes with NPS1 in the GC region of the nucleolus.

Subunit Structure:

Component of the small ribosomal subunit. Interacts with PRMT5. The methylated form interacts with NPM1.

Family&Domains:

Belongs to the eukaryotic ribosomal protein eS10 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

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RPS10 Antibody - #DF9116