HAAO Antibody | Affinity Biosciences

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Western blot analysis of HAAO using Jurkat whole cell lysates.

Product:	HAAO Antibody
Catalog:	DF9115
Description:	Rabbit polyclonal antibody to HAAO
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	33 kDa; 33kD(Calculated).
Uniprot:	P46952
RRID:	AB_2842311

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(92%), Dog(100%)

Clonality:

Polyclonal

Specificity:

HAAO Antibody detects endogenous levels of total HAAO.

RRID:

AB_2842311
Cite Format: Affinity Biosciences Cat# DF9115, RRID:AB_2842311.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

3 HAO; 3 hydroxyanthranilate 3 4 dioxygenase; 3 hydroxyanthranilate dioxygenase; 3 hydroxyanthranilate oxygenase; 3 hydroxyanthranilic acid dioxygenase; 3-HAO; 3-hydroxyanthranilate 3,4-dioxygenase; 3-hydroxyanthranilate oxygenase; 3-hydroxyanthranilic acid dioxygenase; 3HAO_HUMAN; haao; HAD; HAO;

Immunogens

Immunogen:

Uniprot:

P46952(3HAO_HUMAN) >>Visit HPA database.

Gene(ID):

HAAO(23498)

Sequence:

P46952 3HAO_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MERRLGVRAWVKENRGSFQPPVCNKLMHQEQLKVMFIGGPNTRKDYHIEEGEEVFYQLEGDMVLRVLEQGKHRDVVIRQGEIFLLPARVPHSPQRFANTVGLVVERRRLETELDGLRYYVGDTMDVLFEKWFYCKDLGTQLAPIIQEFFSSEQYRTGKPIPDQLLKEPPFPLSTRSIMEPMSLDAWLDSHHRELQAGTPLSLFGDTYETQVIAYGQGSSEGLRQNVDVWLWQLEGSSVVTMGGRRLSLAPDDSLLVLAGTSYAWERTQGSVALSVTQDPACKKPLG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Horse

Rabbit

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P46952 As Substrate

P46952

Site	PTM Type	Source
T111	Phosphorylation	Uniprot
S247	Phosphorylation	Uniprot
S253	Phosphorylation	Uniprot
S270	Phosphorylation	Uniprot
S274	Phosphorylation	Uniprot
T276	Phosphorylation	Uniprot

Research Backgrounds

P46952_3HAO_HUMAN

Function:

Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.

Subcellular Location:

Cytoplasm>Cytosol.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the 3-HAO family.

Research Fields

· Metabolism > Amino acid metabolism > Tryptophan metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

HAAO Antibody - #DF9115