MRPL28 Antibody | Affinity Biosciences

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Western blot analysis of MRPL28 using COS7 whole cell lysates.

Product:	MRPL28 Antibody
Catalog:	DF9105
Description:	Rabbit polyclonal antibody to MRPL28
Application:	WB IHC
Reactivity:	Human, Mouse, Monkey
Prediction:	Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.:	30kDa; 30kD(Calculated).
Uniprot:	Q13084
RRID:	AB_2842301

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Monkey

Prediction:

Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Dog(100%), Chicken(83%)

Clonality:

Polyclonal

Specificity:

MRPL28 Antibody detects endogenous levels of total MRPL28.

RRID:

AB_2842301
Cite Format: Affinity Biosciences Cat# DF9105, RRID:AB_2842301.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

39S ribosomal protein L28; 39S ribosomal protein L28 mitochondrial; 39S ribosomal protein L28 mitochondrial precursor; HGNC6756; L28mt; MAAT 1; MAAT1; Melanoma antigen p15; Melanoma associated antigen recognised by cytotoxic T lymphocytes; Melanoma associated antigen recognized by T lymphocytes; Melanoma-associated antigen recognized by T-lymphocytes; MGC8499; mitochondrial; Mitochondrial ribosomal protein L28; MRP L28; MRP-L28; MRPL 28; mrpl28; P15; RM28_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q13084(RM28_HUMAN) >>Visit HPA database.

Gene(ID):

MRPL28(10573)

Expression:

Q13084 RM28_HUMAN:

Found in a variety of normal tissues including spleen, testes, thymus, liver, kidney, brain, adrenal, lung and retinal tissue.

Sequence:

Q13084 RM28_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPLHKYPVWLWKRLQLREGICSRLPGHYLRSLEEERTPTPVHYRPHGAKFKINPKNGQRERVEDVPIPIYFPPESQRGLWGGEGWILGQIYANNDKLSKRLKKVWKPQLFEREFYSEILDKKFTVTVTMRTLDLIDEAYGLDFYILKTPKEDLCSKFGMDLKRGMLLRLARQDPQLHPEDPERRAAIYDKYKEFAIPEEEAEWVGLTLEEAIEKQRLLEEKDPVPLFKIYVAELIQQLQQQALSEPAVVQKRASGQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Horse

Chicken

Zebrafish

Xenopus

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13084 As Substrate

Q13084

Site	PTM Type	Source
K5	Ubiquitination	Uniprot
Y6	Phosphorylation	Uniprot
K12	Ubiquitination	Uniprot
S31	Phosphorylation	Uniprot
T37	Phosphorylation	Uniprot
T39	Phosphorylation	Uniprot
Y43	Phosphorylation	Uniprot
K106	Ubiquitination	Uniprot
Y115	Phosphorylation	Uniprot
K162	Ubiquitination	Uniprot
K190	Ubiquitination	Uniprot
K214	Ubiquitination	Uniprot
K221	Ubiquitination	Uniprot
K251	Ubiquitination	Uniprot

Research Backgrounds

Q13084_RM28_HUMAN

Subcellular Location:

Mitochondrion.

Tissue Specificity:

Found in a variety of normal tissues including spleen, testes, thymus, liver, kidney, brain, adrenal, lung and retinal tissue.

Subunit Structure:

Component of the mitochondrial large ribosomal subunit (mt-LSU). Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins. Interacts with OXA1L.

Family&Domains:

Belongs to the bacterial ribosomal protein bL28 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

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MRPL28 Antibody - #DF9105