Product: MRPL2 Antibody
Catalog: DF9104
Description: Rabbit polyclonal antibody to MRPL2
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 33 kDa; 33kD(Calculated).
Uniprot: Q5T653
RRID: AB_2842300

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(86%), Bovine(100%), Horse(93%), Sheep(100%), Rabbit(86%), Dog(86%)
Clonality:
Polyclonal
Specificity:
MRPL2 Antibody detects endogenous levels of total MRPL2.
RRID:
AB_2842300
Cite Format: Affinity Biosciences Cat# DF9104, RRID:AB_2842300.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

39S ribosomal protein L2; 39S ribosomal protein L2, mitochondrial precursor; CGI22; L2mt; mitochondrial; Mitochondrial ribosomal protein L2; MRP L14; MRP-L2; MRPL14; Mrpl2; RM02_HUMAN; RPML14;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MALCALTRALRSLNLAPPTVAAPAPSLFPAAQMMNNGLLQQPSALMLLPCRPVLTSVALNANFVSWKSRTKYTITPVKMRKSGGRDHTGRIRVHGIGGGHKQRYRMIDFLRFRPEETKSGPFEEKVIQVRYDPCRSADIALVAGGSRKRWIIATENMQAGDTILNSNHIGRMAVAAREGDAHPLGALPVGTLINNVESEPGRGAQYIRAAGTCGVLLRKVNGTAIIQLPSKRQMQVLETCVATVGRVSNVDHNKRVIGKAGRNRWLGKRPNSGRWHRKGGWAGRKIRPLPPMKSYVKLPSASAQS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Horse
93
Pig
86
Dog
86
Rabbit
86
Zebrafish
79
Chicken
79
Xenopus
77
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5T653 As Substrate

Site PTM Type Enzyme
T7 Phosphorylation
K71 Ubiquitination
Y72 Phosphorylation
K78 Acetylation
K78 Ubiquitination
T117 Phosphorylation
K118 Ubiquitination
S119 Phosphorylation
T223 Phosphorylation
S230 Phosphorylation
T239 Phosphorylation
S248 Phosphorylation
S272 Phosphorylation
K293 Ubiquitination
K297 Sumoylation
K297 Ubiquitination

Research Backgrounds

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the mitochondrial large ribosomal subunit (mt-LSU). Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins.

Family&Domains:

Belongs to the universal ribosomal protein uL2 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

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