Product: MRPS15 Antibody
Catalog: DF9095
Description: Rabbit polyclonal antibody to MRPS15
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Sheep
Mol.Wt.: 30kDa; 30kD(Calculated).
Uniprot: P82914
RRID: AB_2842291

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Sheep(86%)
Clonality:
Polyclonal
Specificity:
MRPS15 Antibody detects endogenous levels of total MRPS15.
RRID:
AB_2842291
Cite Format: Affinity Biosciences Cat# DF9095, RRID:AB_2842291.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

28S ribosomal protein S15; 28S ribosomal protein S15, mitochondrial [Precursor]; DC37; FLJ11564; mitochondrial; mitochondrial ribosomal protein S15; MPR S15; MRP-S15; MRPS15; RPMS15; RT15_HUMAN; S15mt;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MLRVAWRTLSLIRTRAVTQVLVPGLPGGGSAKFPFNQWGLQPRSLLLQAARGYVVRKPAQSRLDDDPPPSTLLKDYQNVPGIEKVDDVVKRLLSLEMANKKEMLKIKQEQFMKKIVANPEDTRSLEARIIALSVKIRSYEEHLEKHRKDKAHKRYLLMSIDQRKKMLKNLRNTNYDVFEKICWGLGIEYTFPPLYYRRAHRRFVTKKALCIRVFQETQKLKKRRRALKAAAAAQKQAKRRNPDSPAKAIPKTLKDSQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
86
Pig
79
Horse
79
Bovine
79
Rabbit
79
Dog
64
Xenopus
64
Zebrafish
64
Chicken
62
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P82914 As Substrate

Site PTM Type Enzyme
K74 Ubiquitination
K84 Ubiquitination
K114 Ubiquitination
S133 Phosphorylation
K145 Ubiquitination
Y189 Phosphorylation
K228 Ubiquitination
S244 Phosphorylation
S256 Phosphorylation

Research Backgrounds

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the mitochondrial small ribosomal subunit (mt-SSU). Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins.

Family&Domains:

Belongs to the universal ribosomal protein uS15 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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