AGPAT7 Antibody | Affinity Biosciences

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Western blot analysis of AGPAT7 using HT29 whole cell lysates.

Product:	AGPAT7 Antibody
Catalog:	DF9092
Description:	Rabbit polyclonal antibody to AGPAT7
Application:	WB
Reactivity:	Human
Prediction:	Rabbit
Mol.Wt.:	57 kDa; 57kD(Calculated).
Uniprot:	Q643R3
RRID:	AB_2842288

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Rabbit(88%)

Clonality:

Polyclonal

Specificity:

AGPAT7 Antibody detects endogenous levels of total AGPAT7.

RRID:

AB_2842288
Cite Format: Affinity Biosciences Cat# DF9092, RRID:AB_2842288.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

1 acyl sn glycerol 3 phosphate acyltransferase eta; 1 AGP acyltransferase 7; 1 AGPAT 7; Acyltransferase like 3; AGPAT7; AYTL3; LPAAT eta; Lpcat4; LPEAT2; Lysophosphatidic acid acyltransferase eta; Lysophosphatidylcholine acyltransferase 4;

Immunogens

Immunogen:

Uniprot:

Q643R3(LPCT4_HUMAN) >>Visit HPA database.

Gene(ID):

LPCAT4(254531)

Expression:

Q643R3 LPCT4_HUMAN:

Widely expressed with predominant level in brain.

Sequence:

Q643R3 LPCT4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSQGSPGDWAPLDPTPGPPASPNPFVHELHLSRLQRVKFCLLGALLAPIRVLLAFIVLFLLWPFAWLQVAGLSEEQLQEPITGWRKTVCHNGVLGLSRLLFFLLGFLRIRVRGQRASRLQAPVLVAAPHSTFFDPIVLLPCDLPKVVSRAENLSVPVIGALLRFNQAILVSRHDPASRRRVVEEVRRRATSGGKWPQVLFFPEGTCSNKKALLKFKPGAFIAGVPVQPVLIRYPNSLDTTSWAWRGPGVLKVLWLTASQPCSIVDVEFLPVYHPSPEESRDPTLYANNVQRVMAQALGIPATECEFVGSLPVIVVGRLKVALEPQLWELGKVLRKAGLSAGYVDAGAEPGRSRMISQEEFARQLQLSDPQTVAGAFGYFQQDTKGLVDFRDVALALAALDGGRSLEELTRLAFELFAEEQAEGPNRLLYKDGFSTILHLLLGSPHPAATALHAELCQAGSSQGLSLCQFQNFSLHDPLYGKLFSTYLRPPHTSRGTSQTPNASSPGNPTALANGTVQAPKQKGD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Horse

Sheep

Dog

Pig

Bovine

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q643R3 As Substrate

Q643R3

Site	PTM Type	Source
S2	Phosphorylation	Uniprot
S97	Phosphorylation	Uniprot
K145	Ubiquitination	Uniprot
K216	Ubiquitination	Uniprot
K319	Ubiquitination	Uniprot
K331	Ubiquitination	Uniprot
K335	Ubiquitination	Uniprot
T496	Phosphorylation	Uniprot
S497	Phosphorylation	Uniprot
T499	Phosphorylation	Uniprot
S503	Phosphorylation	Uniprot

Research Backgrounds

Q643R3_LPCT4_HUMAN

Function:

Displays acyl-CoA-dependent lysophospholipid acyltransferase activity with a subset of lysophospholipids as substrates; converts lysophosphatidylethanolamine to phosphatidylethanolamine, lysophosphatidylcholine to phosphatidycholine, 1-alkenyl-lysophatidylethanolamine to 1-alkenyl-phosphatidylethanolamine, lysophosphatidylglycerol and alkyl-lysophosphatidylcholine to phosphatidylglycerol and alkyl-phosphatidylcholine, respectively. In contrast, has no lysophosphatidylinositol, glycerol-3-phosphate, diacylglycerol or lysophosphatidic acid acyltransferase activity. Prefers long chain acyl-CoAs (C16, C18) as acyl donors.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Tissue Specificity:

Widely expressed with predominant level in brain.

Family&Domains:

Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Research Fields

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

· Metabolism > Lipid metabolism > Ether lipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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AGPAT7 Antibody - #DF9092