ORC6L Antibody | Affinity Biosciences

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Western blot analysis ORC6L using COLO205 whole cell lysates.

Product:	ORC6L Antibody
Catalog:	DF9087
Description:	Rabbit polyclonal antibody to ORC6L
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.:	28 kDa; 28kD(Calculated).
Uniprot:	Q9Y5N6
RRID:	AB_2842283

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(88%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

ORC6L Antibody detects endogenous levels of total ORC6L.

RRID:

AB_2842283
Cite Format: Affinity Biosciences Cat# DF9087, RRID:AB_2842283.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ORC 6; Orc6; ORC6_HUMAN; ORC6L; Origin recognition complex subunit 6; origin recognition complex subunit 6 homolog like; origin recognition complex subunit 6 homolog like (yeast); origin recognition complex subunit 6 like (yeast);

Immunogens

Immunogen:

Uniprot:

Q9Y5N6(ORC6_HUMAN) >>Visit HPA database.

Gene(ID):

ORC6(23594)

Sequence:

Q9Y5N6 ORC6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGSELIGRLAPRLGLAEPDMLRKAEEYLRLSRVKCVGLSARTTETSSAVMCLDLAASWMKCPLDRAYLIKLSGLNKETYQSCLKSFECLLGLNSNIGIRDLAVQFSCIEAVNMASKILKSYESSLPQTQQVDLDLSRPLFTSAALLSACKILKLKVDKNKMVATSGVKKAIFDRLCKQLEKIGQQVDREPGDVATPPRKRKKIVVEAPAKEMEKVEEMPHKPQKDEDLTQDYEEWKRKILENAASAQKATAE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Dog

100

Rabbit

100

Pig

Zebrafish

Xenopus

Sheep

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y5N6 As Substrate

Q9Y5N6

Site	PTM Type	Enzyme	Source
K23	Ubiquitination		Uniprot
K34	Ubiquitination		Uniprot
R65	Methylation		Uniprot
Y67	Phosphorylation		Uniprot
K70	Ubiquitination		Uniprot
K76	Ubiquitination		Uniprot
T78	Phosphorylation		Uniprot
Y79	Phosphorylation		Uniprot
S81	Phosphorylation		Uniprot
K119	Ubiquitination		Uniprot
K168	Acetylation		Uniprot
K168	Ubiquitination		Uniprot
K177	Ubiquitination		Uniprot
K181	Ubiquitination		Uniprot
T195	Phosphorylation	P06493 (CDK1)	Uniprot
K202	Ubiquitination		Uniprot
K210	Acetylation		Uniprot
K210	Ubiquitination		Uniprot
K214	Acetylation		Uniprot
K221	Acetylation		Uniprot
T229	Phosphorylation		Uniprot
Y232	Phosphorylation		Uniprot
K236	Ubiquitination		Uniprot
K238	Ubiquitination		Uniprot
S245	Phosphorylation		Uniprot
K248	Ubiquitination		Uniprot

Research Backgrounds

Q9Y5N6_ORC6_HUMAN

Function:

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Does not bind histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3.

Subcellular Location:

Nucleus.

Subunit Structure:

Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Interacts with DBF4 (By similarity).

Family&Domains:

Belongs to the ORC6 family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)

Restrictive clause

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ORC6L Antibody - #DF9087