ORC2L Antibody - #DF9085
| Product: | ORC2L Antibody |
| Catalog: | DF9085 |
| Description: | Rabbit polyclonal antibody to ORC2L |
| Application: | WB IHC |
| Reactivity: | Human |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 66 kDa; 66kD(Calculated). |
| Uniprot: | Q13416 |
| RRID: | AB_2842281 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9085, RRID:AB_2842281.
Fold/Unfold
orc2; ORC2_HUMAN; ORC2L; Origin of replication 2 (yeast homolog) like; Origin recognition complex protein 2 homolog; Origin recognition complex subunit 2; Origin recognition complex subunit 2 isoform B; Origin recognition complex subunit 2 like (yeast); Origin recognition complex, subunit 2 homolog; Origin recognition complex, subunit 2, S. cerevisiae, homolog of; Origin recognition complex, subunit 2-like (S. cerevisiae);
Immunogens
- Q13416 ORC2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSKPELKEDKMLEVHFVGDDDVLNHILDREGGAKLKKERAQLLVNPKKIIKKPEYDLEEDDQEVLKDQNYVEIMGRDVQESLKNGSATGGGNKVYSFQNRKHSEKMAKLASELAKTPQKSVSFSLKNDPEITINVPQSSKGHSASDKVQPKNNDKSEFLSTAPRSLRKRLIVPRSHSDSESEYSASNSEDDEGVAQEHEEDTNAVIFSQKIQAQNRVVSAPVGKETPSKRMKRDKTSDLVEEYFEAHSSSKVLTSDRTLQKLKRAKLDQQTLRNLLSKVSPSFSAELKQLNQQYEKLFHKWMLQLHLGFNIVLYGLGSKRDLLERFRTTMLQDSIHVVINGFFPGISVKSVLNSITEEVLDHMGTFRSILDQLDWIVNKFKEDSSLELFLLIHNLDSQMLRGEKSQQIIGQLSSLHNIYLIASIDHLNAPLMWDHAKQSLFNWLWYETTTYSPYTEETSYENSLLVKQSGSLPLSSLTHVLRSLTPNARGIFRLLIKYQLDNQDNPSYIGLSFQDFYQQCREAFLVNSDLTLRAQLTEFRDHKLIRTKKGTDGVEYLLIPVDNGTLTDFLEKEEEEA
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13416 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K47 | Acetylation | Uniprot | |
| Y70 | Phosphorylation | Uniprot | |
| Y95 | Phosphorylation | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| T116 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S122 | Phosphorylation | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| K126 | Sumoylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| K147 | Acetylation | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| S179 | Phosphorylation | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| S188 | Phosphorylation | P53350 (PLK1) | Uniprot |
| T202 | Phosphorylation | Uniprot | |
| S219 | Phosphorylation | Uniprot | |
| T226 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S228 | Phosphorylation | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| T236 | Phosphorylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| Y243 | Phosphorylation | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| S249 | Phosphorylation | Uniprot | |
| S250 | Phosphorylation | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| T258 | Phosphorylation | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| K278 | Ubiquitination | Uniprot | |
| S280 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| S475 | Phosphorylation | Uniprot | |
| S483 | Phosphorylation | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| T531 | Phosphorylation | Uniprot | |
| K549 | Ubiquitination | Uniprot |
Research Backgrounds
Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3. Stabilizes LRWD1, by protecting it from ubiquitin-mediated proteasomal degradation. Also stabilizes ORC3.
Nucleus.
Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Interacts with DBF4 (By similarity). Interacts with MCM10. Interacts with LRWD1 throughout the cell cycle; this interaction, wich occurs only with non-ubiquitinated form of LRWD1, prevents LRWD1 ubiquitination and hence stabilizes the protein. Interacts with POLQ.
Belongs to the ORC2 family.
Research Fields
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
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