GEMIN8 Antibody | Affinity Biosciences

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Western blot analysis GEMIN8 using K562 whole cell lysates.

DF9077 at 1/100 staining Rat testis tissue by IHC-P.

Product:	GEMIN8 Antibody
Catalog:	DF9077
Description:	Rabbit polyclonal antibody to GEMIN8
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.:	29kDa; 29kD(Calculated).
Uniprot:	Q9NWZ8
RRID:	AB_2842273

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(90%), Xenopus(80%)

Clonality:

Polyclonal

Specificity:

GEMIN8 Antibody detects endogenous levels of total GEMIN8.

RRID:

AB_2842273
Cite Format: Affinity Biosciences Cat# DF9077, RRID:AB_2842273.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FAM51A1; FLJ20514; FLJ43072; gem (nuclear organelle) associated protein 8;

Immunogens

Immunogen:

Uniprot:

Q9NWZ8(GEMI8_HUMAN) >>Visit HPA database.

Gene(ID):

GEMIN8(54960)

Sequence:

Q9NWZ8 GEMI8_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAVKASTSKATRPWYSHPVYARYWQHYHQAMAWMQSHHNAYRKAVESCFNLPWYLPSALLPQSSYDNEAAYPQSFYDHHVAWQDYPCSSSHFRRSGQHPRYSSRIQASTKEDQALSKEEEMETESDAEVECDLSNMEITEELRQYFAETERHREERRRQQQLDAERLDSYVNADHDLYCNTRRSVEAPTERPGERRQAEMKRLYGDSAAKIQAMEAAVQLSFDKHCDRKQPKYWPVIPLKF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

Xenopus

Chicken

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NWZ8 As Substrate

Q9NWZ8

Site	PTM Type	Source
K5	Ubiquitination	Uniprot
K10	Ubiquitination	Uniprot
Y77	Phosphorylation	Uniprot
S109	Phosphorylation	Uniprot
T110	Phosphorylation	Uniprot
S117	Phosphorylation	Uniprot
T124	Phosphorylation	Uniprot
S126	Phosphorylation	Uniprot
S170	Phosphorylation	Uniprot
Y171	Phosphorylation	Uniprot
Y179	Phosphorylation	Uniprot
T182	Phosphorylation	Uniprot
S185	Phosphorylation	Uniprot
T190	Phosphorylation	Uniprot
Y234	Phosphorylation	Uniprot

Research Backgrounds

Q9NWZ8_GEMI8_HUMAN

Function:

The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus.

Subcellular Location:

Nucleus>Gem. Cytoplasm.
Note: Found in nuclear bodies called gems (Gemini of Cajal bodies) that are often in proximity to Cajal (coiled) bodies. Also found in the cytoplasm.

Subunit Structure:

Part of the core SMN complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Part of the SMN-Sm complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8, STRAP/UNRIP and the Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG. Interacts directly with SMN1 and with the GEMIN6-GEMIN7 heterodimer, mediating their interaction.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

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GEMIN8 Antibody - #DF9077