ERCC2 Antibody - #DF9076
| Product: | ERCC2 Antibody |
| Catalog: | DF9076 |
| Description: | Rabbit polyclonal antibody to ERCC2 |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Zebrafish, Bovine, Sheep, Rabbit, Xenopus |
| Mol.Wt.: | 87kDa; 87kD(Calculated). |
| Uniprot: | P18074 |
| RRID: | AB_2842272 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9076, RRID:AB_2842272.
Fold/Unfold
TFIIH 80 kDa subunit; Basic transcription factor 2 80 kDa subunit; BTF2 p80; COFS 2; COFS2; CXPD; DNA excision repair protein ERCC 2; DNA excision repair protein ERCC-2; DNA repair protein complementing XP D cells; DNA repair protein complementing XP-D cells; EM9; ERCC 2; ERCC2; ERCC2_HUMAN; Excision repair 2; Excision repair cross complementing rodent repair deficiency complementation; Excision repair cross complementing rodent repair deficiency, complementation group 2; MAG; MGC102762; MGC126218; MGC126219; OTTHUMP00000045860; OTTHUMP00000045861; OTTHUMP00000045862; OTTHUMP00000045863; TFIIH 80 kDa subunit; TFIIH basal transcription factor complex 80 kDa subunit; TFIIH Basal Transcription Factor Complex Helicase Subunit; TFIIH basal transcription factor complex helicase XPD subunit; TFIIH basal transcription factor complex p80 subunit; TFIIH p80; TTD; Xeroderma pigmentosum complementary group D; Xeroderma pigmentosum group D complementing protein; Xeroderma pigmentosum group D-complementing protein; XPD; XPDC;
Immunogens
A synthesized peptide derived from human ERCC2, corresponding to a region within C-terminal amino acids.
- P18074 ERCC2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKLNVDGLLVYFPYDYIYPEQFSYMRELKRTLDAKGHGVLEMPSGTGKTVSLLALIMAYQRAYPLEVTKLIYCSRTVPEIEKVIEELRKLLNFYEKQEGEKLPFLGLALSSRKNLCIHPEVTPLRFGKDVDGKCHSLTASYVRAQYQHDTSLPHCRFYEEFDAHGREVPLPAGIYNLDDLKALGRRQGWCPYFLARYSILHANVVVYSYHYLLDPKIADLVSKELARKAVVVFDEAHNIDNVCIDSMSVNLTRRTLDRCQGNLETLQKTVLRIKETDEQRLRDEYRRLVEGLREASAARETDAHLANPVLPDEVLQEAVPGSIRTAEHFLGFLRRLLEYVKWRLRVQHVVQESPPAFLSGLAQRVCIQRKPLRFCAERLRSLLHTLEITDLADFSPLTLLANFATLVSTYAKGFTIIIEPFDDRTPTIANPILHFSCMDASLAIKPVFERFQSVIITSGTLSPLDIYPKILDFHPVTMATFTMTLARVCLCPMIIGRGNDQVAISSKFETREDIAVIRNYGNLLLEMSAVVPDGIVAFFTSYQYMESTVASWYEQGILENIQRNKLLFIETQDGAETSVALEKYQEACENGRGAILLSVARGKVSEGIDFVHHYGRAVIMFGVPYVYTQSRILKARLEYLRDQFQIRENDFLTFDAMRHAAQCVGRAIRGKTDYGLMVFADKRFARGDKRGKLPRWIQEHLTDANLNLTVDEGVQVAKYFLRQMAQPFHREDQLGLSLLSLEQLESEETLKRIEQIAQQL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
Research Backgrounds
ATP-dependent 5'-3' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATP-dependent helicase activity of XPD/ERCC2 is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. XPD/ERCC2 acts by forming a bridge between CAK and the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers.
ISGylated.
Nucleus. Cytoplasm>Cytoskeleton>Spindle.
Belongs to the helicase family. RAD3/XPD subfamily.
Research Fields
· Genetic Information Processing > Transcription > Basal transcription factors.
· Genetic Information Processing > Replication and repair > Nucleotide excision repair.
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