RFC5 Antibody - #DF9064

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Product: RFC5 Antibody
Catalog: DF9064
Description: Rabbit polyclonal antibody to RFC5
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 36 kDa; 38kD(Calculated).
Uniprot: P40937
RRID: AB_2842260

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(92%), Zebrafish(83%), Bovine(92%), Horse(88%), Sheep(92%), Rabbit(92%), Dog(92%), Chicken(92%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
RFC5 Antibody detects endogenous levels of total RFC5.
RRID:
AB_2842260
Cite Format: Affinity Biosciences Cat# DF9064, RRID:AB_2842260.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

36 kDa subunit; A1 36; A1 36 kDa subunit; Activator 1 36 kDa subunit; Activator 1; Activator 1 subunit 5; MGC1155; Replication factor C (activator 1) 5 (36.5kD); Replication factor C 36 kDa subunit; Replication factor C 5 isoform 1; Replication Factor C; Replication factor C subunit 5; replication factor C, 36-kDa subunit; RF-C 36 kDa subunit; RFC 36.5 kD subunit; RFC 5; RFC3, yeast, homolog of; RFC36; rfc5; RFC5_HUMAN;

Immunogens

Immunogen:
Uniprot:

P40937(RFC5_HUMAN) >>Visit HPA database.

Gene(ID):
RFC5(5985)
Sequence:
METSALKQQEQPAATKIRNLPWVEKYRPQTLNDLISHQDILSTIQKFINEDRLPHLLLYGPPGTGKTSTILACAKQLYKDKEFGSMVLELNASDDRGIDIIRGPILSFASTRTIFKKGFKLVILDEADAMTQDAQNALRRVIEKFTENTRFCLICNYLSKIIPALQSRCTRFRFGPLTPELMVPRLEHVVEEEKVDISEDGMKALVTLSSGDMRRALNILQSTNMAFGKVTEETVYTCTGHPLKSDIANILDWMLNQDFTTAYRNITELKTLKGLALHDILTEIHLFVHRVDFPSSVRIHLLTKMADIEYRLSVGTNEKIQLSSLIAAFQVTRDLIVAEA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Bovine
92
Sheep
92
Dog
92
Xenopus
92
Chicken
92
Rabbit
92
Horse
88
Zebrafish
83
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P40937 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K7 Ubiquitination
K16 Ubiquitination
K25 Ubiquitination
R52 Methylation
K66 Ubiquitination
K75 Ubiquitination
R96 Methylation
K120 Ubiquitination
K144 Ubiquitination
S159 Phosphorylation
T178 Phosphorylation
K194 Ubiquitination
K203 Ubiquitination
T231 Phosphorylation
T234 Phosphorylation
Y236 Phosphorylation
T237 Phosphorylation
C238 S-Nitrosylation
K270 Ubiquitination
K304 Ubiquitination
S313 Phosphorylation

Research Backgrounds

Function:

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex either with RFC1 or with RAD17. The former interacts with PCNA in the presence of ATP, while the latter has ATPase activity but is not stimulated by PCNA.

Family&Domains:

Belongs to the activator 1 small subunits family.

Research Fields

· Genetic Information Processing > Replication and repair > DNA replication.

· Genetic Information Processing > Replication and repair > Nucleotide excision repair.

· Genetic Information Processing > Replication and repair > Mismatch repair.

Restrictive clause

 

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For Research Use Only.
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