ACAA2 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from HepG2 cells, using ACAA2 Antibody.

Product:	ACAA2 Antibody
Catalog:	DF9062
Description:	Rabbit polyclonal antibody to ACAA2
Application:	WB IHC IF/ICC
Reactivity:	Human, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	42 kDa; 42kD(Calculated).
Uniprot:	P42765
RRID:	AB_2842258

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Pig(83%), Zebrafish(83%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(92%), Dog(92%), Chicken(92%), Xenopus(83%)

Clonality:

Polyclonal

Specificity:

ACAA2 Antibody detects endogenous levels of total ACAA2.

RRID:

AB_2842258
Cite Format: Affinity Biosciences Cat# DF9062, RRID:AB_2842258.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

3 ketoacyl CoA thiolase; 3 ketoacyl CoA thiolase mitochondrial; 3-ketoacyl-CoA thiolase, mitochondrial; 3-oxoacyl-CoA thiolase, mitochondrial; ACAA 2; Acaa2; Acetyl CoA acyltransferase 2; Acetyl CoA acyltransferase; Acetyl Coenzyme A acyltransferase 2; Acetyl-CoA acyltransferase; Beta ketothiolase; Beta-ketothiolase; DSAEC; Mitochondrial 3 oxoacyl CoA thiolase; Mitochondrial 3 oxoacyl Coenzyme A thiolase; Mitochondrial 3-oxoacyl-CoA thiolase; T1; THIM_HUMAN;

Immunogens

Immunogen:

Uniprot:

P42765(THIM_HUMAN) >>Visit HPA database.

Gene(ID):

ACAA2(10449)

Sequence:

P42765 THIM_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALLRGVFVVAAKRTPFGAYGGLLKDFTATDLSEFAAKAALSAGKVSPETVDSVIMGNVLQSSSDAIYLARHVGLRVGIPKETPALTINRLCGSGFQSIVNGCQEICVKEAEVVLCGGTESMSQAPYCVRNVRFGTKLGSDIKLEDSLWVSLTDQHVQLPMAMTAENLAVKHKISREECDKYALQSQQRWKAANDAGYFNDEMAPIEVKTKKGKQTMQVDEHARPQTTLEQLQKLPPVFKKDGTVTAGNASGVADGAGAVIIASEDAVKKHNFTPLARIVGYFVSGCDPSIMGIGPVPAISGALKKAGLSLKDMDLVEVNEAFAPQYLAVERSLDLDISKTNVNGGAIALGHPLGGSGSRITAHLVHELRRRGGKYAVGSACIGGGQGIAVIIQSTA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Bovine

Sheep

Dog

Chicken

Rabbit

Pig

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P42765 As Substrate

P42765

Site	PTM Type	Source
K13	Acetylation	Uniprot
Y20	Phosphorylation	Uniprot
K25	Acetylation	Uniprot
K38	Acetylation	Uniprot
K38	Ubiquitination	Uniprot
Y68	Phosphorylation	Uniprot
K81	Acetylation	Uniprot
K81	Ubiquitination	Uniprot
T87	Phosphorylation	Uniprot
T119	Phosphorylation	Uniprot
S121	Phosphorylation	Uniprot
Y127	Phosphorylation	Uniprot
K137	Acetylation	Uniprot
K137	Ubiquitination	Uniprot
S140	Phosphorylation	Uniprot
K181	Acetylation	Uniprot
K181	Ubiquitination	Uniprot
Y182	Phosphorylation	Uniprot
K191	Ubiquitination	Uniprot
Y198	Phosphorylation	Uniprot
K234	Acetylation	Uniprot
K234	Ubiquitination	Uniprot
K240	Acetylation	Uniprot
K241	Acetylation	Uniprot
K241	Ubiquitination	Uniprot
K269	Acetylation	Uniprot
K269	Ubiquitination	Uniprot
K270	Acetylation	Uniprot
T274	Phosphorylation	Uniprot
K305	Acetylation	Uniprot
K306	Acetylation	Uniprot
S310	Phosphorylation	Uniprot
S333	Phosphorylation	Uniprot
S339	Phosphorylation	Uniprot
R370	Methylation	Uniprot

Research Backgrounds

P42765_THIM_HUMAN

Function:

In the production of energy from fats, this is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA (Probable). Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain unbranched 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms (Probable). Also catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA and could be involved in the production of ketone bodies (Probable). Also displays hydrolase activity on various fatty acyl-CoAs. Thereby, could be responsible for the production of acetate in a side reaction to beta-oxidation (Probable). Abolishes BNIP3-mediated apoptosis and mitochondrial damage.

Subcellular Location:

Mitochondrion.

Subunit Structure:

Homotetramer. Interacts with BNIP3.

Family&Domains:

Belongs to the thiolase-like superfamily. Thiolase family.

Research Fields

· Metabolism > Lipid metabolism > Fatty acid elongation.

· Metabolism > Lipid metabolism > Fatty acid degradation.

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Fatty acid metabolism.

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ACAA2 Antibody - #DF9062