RAD54B Antibody - #DF9055

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Product: RAD54B Antibody
Catalog: DF9055
Description: Rabbit polyclonal antibody to RAD54B
Application: WB
Reactivity: Human
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 102 kDa; 103kD(Calculated).
Uniprot: Q9Y620
RRID: AB_2842251

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(91%), Zebrafish(83%), Bovine(82%), Sheep(91%), Rabbit(91%), Dog(89%)
Clonality:
Polyclonal
Specificity:
RAD54B Antibody detects endogenous levels of total RAD54B.
RRID:
AB_2842251
Cite Format: Affinity Biosciences Cat# DF9055, RRID:AB_2842251.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DNA repair and recombination protein RAD54B; EC 3.6.1.-; Fibrinogen silencer binding protein; FSBP; RAD54 homolog B;

Immunogens

Immunogen:
Uniprot:

Q9Y620(RA54B_HUMAN) >>Visit HPA database.

Gene(ID):
RAD54B(25788)
Expression:
Q9Y620 RA54B_HUMAN:

Abundantly expressed in testis and spleen. Relatively low levels observed in thymus, prostate, ovary and colon.

Sequence:
MRRSAAPSQLQGNSFKKPKFIPPGRSNPGLNEEITKLNPDIKLFEGVAINNTFLPSQNDLRICSLNLPSEESTREINNRDNCSGKYCFEAPTLATLDPPHTVHSAPKEVAVSKEQEEKSDSLVKYFSVVWCKPSKKKHKKWEGDAVLIVKGKSFILKNLEGKDIGRGIGYKFKELEKIEEGQTLMICGKEIEVMGVISPDDFSSGRCFQLGGGSTAISHSSQVARKCFSNPFKSVCKPSSKENRQNDFQNCKPRHDPYTPNSLVMPRPDKNHQWVFNKNCFPLVDVVIDPYLVYHLRPHQKEGIIFLYECVMGMRMNGRCGAILADEMGLGKTLQCISLIWTLQCQGPYGGKPVIKKTLIVTPGSLVNNWKKEFQKWLGSERIKIFTVDQDHKVEEFIKSIFYSVLIISYEMLLRSLDQIKNIKFDLLICDEGHRLKNSAIKTTTALISLSCEKRIILTGTPIQNDLQEFFALIDFVNPGILGSLSSYRKIYEEPIILSREPSASEEEKELGERRAAELTCLTGLFILRRTQEIINKYLPPKIENVVFCRPGALQIELYRKLLNSQVVRFCLQGLLENSPHLICIGALKKLCNHPCLLFNSIKEKECSSTCDKNEEKSLYKGLLSVFPADYNPLLFTEKESGKLQVLSKLLAVIHELRPTEKVVLVSNYTQTLNILQEVCKRHGYAYTRLDGQTPISQRQQIVDGFNSQHSSFFIFLLSSKAGGVGLNLIGGSHLILYDIDWNPATDIQAMSRVWRDGQKYPVHIYRLLTTGTIEEKIYQRQISKQGLCGAVVDLTKTSEHIQFSVEELKNLFTLHESSDCVTHDLLDCECTGEEVHTGDSLEKFIVSRDCQLGPHHQKSNSLKPLSMSQLKQWKHFSGDHLNLTDPFLERITENVSFIFQNITTQATGT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Sheep
91
Rabbit
91
Dog
89
Zebrafish
83
Bovine
82
Chicken
75
Xenopus
70
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y620 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
S14 Phosphorylation
K36 Ubiquitination
K157 Ubiquitination
K162 Ubiquitination
K226 Methylation
K226 Ubiquitination
S229 Phosphorylation
K252 Ubiquitination
K270 Ubiquitination
K371 Ubiquitination
K376 Ubiquitination
K384 Ubiquitination
S409 Phosphorylation
K490 Ubiquitination
Y492 Phosphorylation
K590 Ubiquitination
K603 Ubiquitination
S608 Phosphorylation
S609 Phosphorylation
T610 Phosphorylation
K621 Ubiquitination
K639 Ubiquitination
K643 Acetylation
K643 Ubiquitination
S648 Phosphorylation
Y761 Phosphorylation
K777 Ubiquitination
K785 Ubiquitination
K864 Ubiquitination
K872 Ubiquitination
K875 Ubiquitination

Research Backgrounds

Function:

Involved in DNA repair and mitotic recombination. May play an active role in recombination processes in concert with other members of the RAD52 epistasis group.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Abundantly expressed in testis and spleen. Relatively low levels observed in thymus, prostate, ovary and colon.

Subunit Structure:

Interacts with RAD51 through the NH2-terminal domain. Immunoprecipitation experiments show that the interaction is constitutive and not induced by ionizing radiation. The interaction may be indirect.

Family&Domains:

Belongs to the SNF2/RAD54 helicase family.

Research Fields

· Genetic Information Processing > Replication and repair > Homologous recombination.

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