ACINU Antibody | Affinity Biosciences

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Western blot analysis of extracts from Huvec, using ACINU Antibody.

Western blot analysis of extracts from 293 cells(UV treatment), using ACINU Antibody.

Product:	ACINU Antibody
Catalog:	DF9051
Description:	Rabbit polyclonal antibody to ACINU
Application:	WB
Reactivity:	Human, Mouse, Rat, Monkey
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	152 kDa; 152kD(Calculated).
Uniprot:	Q9UKV3
RRID:	AB_2842247

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Prediction:

Pig(100%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

ACINU Antibody detects endogenous levels of total ACINU.

RRID:

AB_2842247
Cite Format: Affinity Biosciences Cat# DF9051, RRID:AB_2842247.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ACIN 1; ACIN1; ACINU_HUMAN; Acinus; ACN; Apoptotic chromatin condensation inducer 1; Apoptotic chromatin condensation inducer in the nucleus; DKFZp667N107; fSAP152; functional spliceosome-associated protein 152; KIAA0670;

Immunogens

Immunogen:

Uniprot:

Q9UKV3(ACINU_HUMAN) >>Visit HPA database.

Gene(ID):

ACIN1(22985)

Expression:

Q9UKV3 ACINU_HUMAN:

Ubiquitous. The Ser-1180 phosphorylated form (by SRPK2) is highly expressed and phosphorylated in patients with myeloid hematologic malignancies.

Sequence:

Q9UKV3 ACINU_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MWRRKHPRTSGGTRGVLSGNRGVEYGSGRGHLGTFEGRWRKLPKMPEAVGTDPSTSRKMAELEEVTLDGKPLQALRVTDLKAALEQRGLAKSGQKSALVKRLKGALMLENLQKHSTPHAAFQPNSQIGEEMSQNSFIKQYLEKQQELLRQRLEREAREAAELEEASAESEDEMIHPEGVASLLPPDFQSSLERPELELSRHSPRKSSSISEEKGDSDDEKPRKGERRSSRVRQARAAKLSEGSQPAEEEEDQETPSRNLRVRADRNLKTEEEEEEEEEEEEDDEEEEGDDEGQKSREAPILKEFKEEGEEIPRVKPEEMMDERPKTRSQEQEVLERGGRFTRSQEEARKSHLARQQQEKEMKTTSPLEEEEREIKSSQGLKEKSKSPSPPRLTEDRKKASLVALPEQTASEEETPPPLLTKEASSPPPHPQLHSEEEIEPMEGPAPAVLIQLSPPNTDADTRELLVSQHTVQLVGGLSPLSSPSDTKAESPAEKVPEESVLPLVQKSTLADYSAQKDLEPESDRSAQPLPLKIEELALAKGITEECLKQPSLEQKEGRRASHTLLPSHRLKQSADSSSSRSSSSSSSSSRSRSRSPDSSGSRSHSPLRSKQRDVAQARTHANPRGRPKMGSRSTSESRSRSRSRSRSASSNSRKSLSPGVSRDSSTSYTETKDPSSGQEVATPPVPQLQVCEPKERTSTSSSSVQARRLSQPESAEKHVTQRLQPERGSPKKCEAEEAEPPAATQPQTSETQTSHLPESERIHHTVEEKEEVTMDTSENRPENDVPEPPMPIADQVSNDDRPEGSVEDEEKKESSLPKSFKRKISVVSATKGVPAGNSDTEGGQPGRKRRWGASTATTQKKPSISITTESLKSLIPDIKPLAGQEAVVDLHADDSRISEDETERNGDDGTHDKGLKICRTVTQVVPAEGQENGQREEEEEEKEPEAEPPVPPQVSVEVALPPPAEHEVKKVTLGDTLTRRSISQQKSGVSITIDDPVRTAQVPSPPRGKISNIVHISNLVRPFTLGQLKELLGRTGTLVEEAFWIDKIKSHCFVTYSTVEEAVATRTALHGVKWPQSNPKFLCADYAEQDELDYHRGLLVDRPSETKTEEQGIPRPLHPPPPPPVQPPQHPRAEQREQERAVREQWAEREREMERRERTRSEREWDRDKVREGPRSRSRSRDRRRKERAKSKEKKSEKKEKAQEEPPAKLLDDLFRKTKAAPCIYWLPLTDSQIVQKEAERAERAKEREKRRKEQEEEEQKEREKEAERERNRQLEREKRREHSRERDRERERERERDRGDRDRDRERDRERGRERDRRDTKRHSRSRSRSTPVRDRGGRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Dog

100

Rabbit

100

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UKV3 As Substrate

Q9UKV3

Site	PTM Type	Enzyme	Source
K5	Ubiquitination		Uniprot
R8	Methylation		Uniprot
R14	Methylation		Uniprot
S18	Phosphorylation		Uniprot
R29	Methylation		Uniprot
K81	Methylation		Uniprot
K81	Ubiquitination		Uniprot
R87	Methylation		Uniprot
S96	Phosphorylation		Uniprot
K103	Methylation		Uniprot
K103	Ubiquitination		Uniprot
K113	Methylation		Uniprot
S115	Phosphorylation		Uniprot
T116	Phosphorylation		Uniprot
S125	Phosphorylation		Uniprot
S132	Phosphorylation		Uniprot
S135	Phosphorylation		Uniprot
K138	Ubiquitination		Uniprot
K143	Methylation		Uniprot
K143	Ubiquitination		Uniprot
S166	Phosphorylation		Uniprot
S169	Phosphorylation		Uniprot
S189	Phosphorylation		Uniprot
S190	Phosphorylation		Uniprot
S199	Phosphorylation		Uniprot
S206	Phosphorylation		Uniprot
S208	Phosphorylation		Uniprot
S210	Phosphorylation		Uniprot
S216	Phosphorylation		Uniprot
S228	Phosphorylation		Uniprot
S229	Phosphorylation		Uniprot
K238	Acetylation		Uniprot
S240	Phosphorylation		Uniprot
S243	Phosphorylation		Uniprot
T254	Phosphorylation		Uniprot
S256	Phosphorylation		Uniprot
K268	Sumoylation		Uniprot
T269	Phosphorylation		Uniprot
S295	Phosphorylation		Uniprot
K302	Ubiquitination		Uniprot
K315	Sumoylation		Uniprot
T326	Phosphorylation		Uniprot
S328	Phosphorylation		Uniprot
R336	Methylation		Uniprot
T341	Phosphorylation		Uniprot
S343	Phosphorylation		Uniprot
K359	Acetylation		Uniprot
T363	Phosphorylation		Uniprot
T364	Phosphorylation		Uniprot
S365	Phosphorylation		Uniprot
S376	Phosphorylation		Uniprot
S377	Phosphorylation		Uniprot
S384	Phosphorylation		Uniprot
S386	Phosphorylation		Uniprot
S388	Phosphorylation		Uniprot
T393	Phosphorylation		Uniprot
S400	Phosphorylation		Uniprot
T408	Phosphorylation		Uniprot
S410	Phosphorylation		Uniprot
T414	Phosphorylation		Uniprot
T420	Phosphorylation		Uniprot
S424	Phosphorylation		Uniprot
S425	Phosphorylation		Uniprot
S434	Phosphorylation		Uniprot
S453	Phosphorylation		Uniprot
T457	Phosphorylation		Uniprot
T461	Phosphorylation		Uniprot
S478	Phosphorylation		Uniprot
S481	Phosphorylation		Uniprot
S482	Phosphorylation		Uniprot
S484	Phosphorylation		Uniprot
T486	Phosphorylation		Uniprot
S490	Phosphorylation		Uniprot
S499	Phosphorylation		Uniprot
K506	Ubiquitination		Uniprot
Y512	Phosphorylation		Uniprot
S513	Phosphorylation		Uniprot
K516	Ubiquitination		Uniprot
S522	Phosphorylation		Uniprot
R524	Methylation		Uniprot
S525	Phosphorylation		Uniprot
K532	Sumoylation		Uniprot
K540	Ubiquitination		Uniprot
K548	Ubiquitination		Uniprot
S551	Phosphorylation		Uniprot
S561	Phosphorylation		Uniprot
T563	Phosphorylation		Uniprot
S583	Phosphorylation		Uniprot
S584	Phosphorylation		Uniprot
S585	Phosphorylation		Uniprot
S586	Phosphorylation		Uniprot
S588	Phosphorylation		Uniprot
S595	Phosphorylation		Uniprot
S599	Phosphorylation		Uniprot
S601	Phosphorylation		Uniprot
S605	Phosphorylation		Uniprot
S635	Phosphorylation		Uniprot
S637	Phosphorylation		Uniprot
S645	Phosphorylation		Uniprot
K654	Methylation		Uniprot
S655	Phosphorylation		Uniprot
S657	Phosphorylation		Uniprot
S661	Phosphorylation		Uniprot
S664	Phosphorylation		Uniprot
T666	Phosphorylation		Uniprot
Y668	Phosphorylation		Uniprot
T671	Phosphorylation		Uniprot
S675	Phosphorylation		Uniprot
S676	Phosphorylation		Uniprot
T682	Phosphorylation		Uniprot
C691	S-Nitrosylation		Uniprot
T697	Phosphorylation		Uniprot
S698	Phosphorylation		Uniprot
T699	Phosphorylation		Uniprot
S702	Phosphorylation		Uniprot
S703	Phosphorylation		Uniprot
S710	Phosphorylation		Uniprot
S714	Phosphorylation		Uniprot
K717	Acetylation		Uniprot
T720	Phosphorylation		Uniprot
S729	Phosphorylation		Uniprot
T744	Phosphorylation		Uniprot
T748	Phosphorylation		Uniprot
T751	Phosphorylation		Uniprot
S754	Phosphorylation		Uniprot
S814	Phosphorylation		Uniprot
S815	Phosphorylation		Uniprot
K818	Ubiquitination		Uniprot
S819	Phosphorylation		Uniprot
K823	Ubiquitination		Uniprot
S825	Phosphorylation		Uniprot
S828	Phosphorylation		Uniprot
T830	Phosphorylation		Uniprot
K831	Ubiquitination		Uniprot
S838	Phosphorylation		Uniprot
T840	Phosphorylation		Uniprot
K860	Ubiquitination		Uniprot
K861	Ubiquitination		Uniprot
S863	Phosphorylation		Uniprot
S865	Phosphorylation		Uniprot
S895	Phosphorylation		Uniprot
S898	Phosphorylation		Uniprot
T902	Phosphorylation		Uniprot
T976	Phosphorylation		Uniprot
T978	Phosphorylation		Uniprot
S981	Phosphorylation		Uniprot
S983	Phosphorylation		Uniprot
K986	Ubiquitination		Uniprot
S987	Phosphorylation		Uniprot
S990	Phosphorylation		Uniprot
T992	Phosphorylation		Uniprot
T999	Phosphorylation		Uniprot
S1004	Phosphorylation		Uniprot
K1029	Ubiquitination		Uniprot
K1047	Ubiquitination		Uniprot
S1104	Phosphorylation		Uniprot
T1106	Phosphorylation		Uniprot
S1161	Phosphorylation		Uniprot
S1176	Phosphorylation		Uniprot
S1178	Phosphorylation		Uniprot
S1180	Phosphorylation	P78362 (SRPK2) , P31749 (AKT1)	Uniprot
K1209	Ubiquitination		Uniprot
K1219	Ubiquitination		Uniprot
Y1225	Phosphorylation		Uniprot
T1230	Phosphorylation		Uniprot
S1232	Phosphorylation		Uniprot
K1237	Ubiquitination		Uniprot
K1253	Methylation		Uniprot
S1284	Phosphorylation		Uniprot
S1325	Phosphorylation		Uniprot
S1327	Phosphorylation		Uniprot
S1329	Phosphorylation	P31749 (AKT1)	Uniprot
S1331	Phosphorylation		Uniprot
T1332	Phosphorylation		Uniprot

Research Backgrounds

Q9UKV3_ACINU_HUMAN

Function:

Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets; ACIN1 confers RNA-binding to the complex. The ASAP complex can inhibit RNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function. Induces apoptotic chromatin condensation after activation by CASP3. Regulates cyclin A1, but not cyclin A2, expression in leukemia cells.

PTMs:

Phosphorylation on Ser-1180 by SRPK2 up-regulates its stimulatory effect on cyclin A1.

Undergoes proteolytic cleavage; the processed form is active, contrary to the uncleaved form.

Subcellular Location:

Nucleus. Nucleus speckle. Nucleus>Nucleoplasm.
Note: Phosphorylation on Ser-1180 by SRPK2 redistributes it from the nuclear speckles to the nucleoplasm.

Tissue Specificity:

Ubiquitous. The Ser-1180 phosphorylated form (by SRPK2) is highly expressed and phosphorylated in patients with myeloid hematologic malignancies.

Subunit Structure:

Found in a mRNA splicing-dependent exon junction complex (EJC). Component of the heterotrimeric ASAP (apoptosis- and splicing-associated protein) complexes consisting of RNPS1, SAP18 and different isoforms of ACIN1; the association of SAP18 seems to require a preformed RNPS1:ACIN1 complex. Interacts with API5. Interacts with SRPK2 in a phosphorylation-dependent manner.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

· Genetic Information Processing > Translation > mRNA surveillance pathway.

· Genetic Information Processing > Transcription > Spliceosome.

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ACINU Antibody - #DF9051